Experiment: Def Vancomycin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0011 and Psest_0012 are separated by 107 nucleotides Psest_0012 and Psest_0013 are separated by 108 nucleotides
Psest_0011: Psest_0011 - glycyl-tRNA synthetase, tetrameric type, alpha subunit, at 13,407 to 14,354
_0011
Psest_0012: Psest_0012 - DNA-3-methyladenine glycosylase I, at 14,462 to 15,019
_0012
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Position (kb) 14
15
16 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 14.395 kb on - strand at 14.395 kb on - strand at 14.395 kb on - strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.499 kb on - strand at 14.499 kb on - strand at 14.936 kb on + strand, within Psest_0012 at 14.936 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.994 kb on - strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.034 kb on - strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand at 15.518 kb on + strand, within Psest_0013 at 15.518 kb on + strand, within Psest_0013 at 15.519 kb on - strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.723 kb on + strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.932 kb on + strand at 15.932 kb on + strand at 15.932 kb on + strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.944 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.008 mg/ml remove 14,395 - -0.6 14,395 - -0.1 14,395 - -1.6 14,498 + -1.0 14,498 + -0.3 14,498 + +0.2 14,498 + +0.0 14,498 + -0.3 14,499 - +0.7 14,499 - -0.1 14,936 + Psest_0012 0.85 -1.4 14,936 + Psest_0012 0.85 -1.4 14,942 + Psest_0012 0.86 -1.1 14,942 + Psest_0012 0.86 -0.0 14,942 + Psest_0012 0.86 +0.1 14,942 + Psest_0012 0.86 +0.1 14,942 + Psest_0012 0.86 +0.6 14,942 + Psest_0012 0.86 +0.3 14,942 + Psest_0012 0.86 -0.2 14,942 + Psest_0012 0.86 -1.7 14,942 + Psest_0012 0.86 +1.2 14,942 + Psest_0012 0.86 -0.3 14,942 + Psest_0012 0.86 +0.5 14,942 + Psest_0012 0.86 +0.2 14,943 - Psest_0012 0.86 -1.1 14,943 - Psest_0012 0.86 +0.1 14,943 - Psest_0012 0.86 -0.9 14,943 - Psest_0012 0.86 +0.9 14,943 - Psest_0012 0.86 -0.9 14,943 - Psest_0012 0.86 +1.0 14,994 - +0.9 15,033 + +0.6 15,033 + +0.6 15,033 + +0.1 15,034 - -0.1 15,094 + +0.1 15,094 + +0.7 15,094 + +0.1 15,095 - -0.5 15,518 + Psest_0013 0.44 -1.1 15,518 + Psest_0013 0.44 -3.1 15,519 - Psest_0013 0.44 -3.4 15,548 + Psest_0013 0.47 -2.0 15,548 + Psest_0013 0.47 -0.4 15,549 - Psest_0013 0.47 -1.9 15,549 - Psest_0013 0.47 -1.1 15,723 + Psest_0013 0.67 -3.4 15,724 - Psest_0013 0.67 -2.4 15,724 - Psest_0013 0.67 -1.8 15,724 - Psest_0013 0.67 -1.7 15,878 + Psest_0013 0.84 -1.3 15,878 + Psest_0013 0.84 -2.1 15,878 + Psest_0013 0.84 -0.1 15,878 + Psest_0013 0.84 -2.8 15,878 + Psest_0013 0.84 -0.4 15,878 + Psest_0013 0.84 -2.4 15,878 + Psest_0013 0.84 -2.7 15,878 + Psest_0013 0.84 -2.7 15,878 + Psest_0013 0.84 -1.8 15,878 + Psest_0013 0.84 -1.9 15,878 + Psest_0013 0.84 -1.7 15,879 - Psest_0013 0.85 -3.6 15,879 - Psest_0013 0.85 -0.1 15,879 - Psest_0013 0.85 -2.3 15,879 - Psest_0013 0.85 -2.8 15,879 - Psest_0013 0.85 -1.4 15,879 - Psest_0013 0.85 -3.1 15,879 - Psest_0013 0.85 -0.4 15,879 - Psest_0013 0.85 -1.7 15,932 + -3.0 15,932 + -3.2 15,932 + -1.6 15,933 - -0.7 15,933 - -3.1 15,933 - -3.0 15,933 - -2.6 15,944 + +0.3 15,956 + -2.1 15,956 + -1.1 15,956 + -2.1 15,956 + -2.1 15,956 + -1.7 15,956 + -0.4 15,956 + -2.4 15,956 + -3.1 15,956 + -3.7 15,956 + -0.7 15,956 + -2.6 15,956 + -0.1 15,956 + -1.1 15,956 + -3.9 15,956 + -2.3 15,956 + -0.1 15,956 + -0.4 15,957 - -0.4 15,957 - -1.1 15,957 - -2.4 15,957 - +0.3 15,957 - -3.4 15,957 - -1.3 15,957 - +0.9
Or see this region's nucleotide sequence