Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1024 and Psest_1025 overlap by 1 nucleotides Psest_1025 and Psest_1026 are separated by 11 nucleotides Psest_1026 and Psest_1028 are separated by 205 nucleotides
Psest_1024: Psest_1024 - ABC-type Fe3+-siderophore transport system, permease component, at 1,110,560 to 1,111,597
_1024
Psest_1025: Psest_1025 - ABC-type hemin transport system, ATPase component, at 1,111,597 to 1,112,364
_1025
Psest_1026: Psest_1026 - Uncharacterized iron-regulated protein, at 1,112,376 to 1,113,260
_1026
Psest_1028: Psest_1028 - Cyclic nucleotide-binding domain., at 1,113,466 to 1,114,263
_1028
Position (kb)
1112
1113
1114 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1111.416 kb on + strand, within Psest_1024 at 1111.417 kb on - strand, within Psest_1024 at 1111.572 kb on - strand at 1111.572 kb on - strand at 1111.618 kb on - strand at 1111.808 kb on + strand, within Psest_1025 at 1111.808 kb on + strand, within Psest_1025 at 1111.808 kb on + strand, within Psest_1025 at 1111.809 kb on - strand, within Psest_1025 at 1111.983 kb on + strand, within Psest_1025 at 1111.983 kb on + strand, within Psest_1025 at 1111.983 kb on + strand, within Psest_1025 at 1111.983 kb on + strand, within Psest_1025 at 1111.983 kb on + strand, within Psest_1025 at 1111.984 kb on - strand, within Psest_1025 at 1111.984 kb on - strand, within Psest_1025 at 1111.984 kb on - strand, within Psest_1025 at 1111.984 kb on - strand, within Psest_1025 at 1111.987 kb on + strand, within Psest_1025 at 1112.072 kb on + strand, within Psest_1025 at 1112.072 kb on + strand, within Psest_1025 at 1112.072 kb on + strand, within Psest_1025 at 1112.073 kb on - strand, within Psest_1025 at 1112.073 kb on - strand, within Psest_1025 at 1112.073 kb on - strand, within Psest_1025 at 1112.075 kb on + strand, within Psest_1025 at 1112.389 kb on + strand at 1112.389 kb on + strand at 1112.390 kb on - strand at 1112.390 kb on - strand at 1112.390 kb on - strand at 1112.516 kb on + strand, within Psest_1026 at 1112.516 kb on + strand, within Psest_1026 at 1112.550 kb on + strand, within Psest_1026 at 1112.551 kb on - strand, within Psest_1026 at 1112.657 kb on - strand, within Psest_1026 at 1112.784 kb on + strand, within Psest_1026 at 1113.136 kb on + strand, within Psest_1026 at 1113.193 kb on - strand at 1113.202 kb on - strand at 1113.203 kb on + strand at 1113.203 kb on + strand at 1113.204 kb on - strand at 1113.204 kb on - strand at 1113.274 kb on + strand at 1113.274 kb on + strand at 1113.275 kb on - strand at 1113.275 kb on - strand at 1113.694 kb on + strand, within Psest_1028 at 1113.694 kb on + strand, within Psest_1028 at 1113.694 kb on + strand, within Psest_1028 at 1113.695 kb on - strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.754 kb on + strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.755 kb on - strand, within Psest_1028 at 1113.930 kb on + strand, within Psest_1028 at 1113.965 kb on - strand, within Psest_1028 at 1114.049 kb on + strand, within Psest_1028 at 1114.049 kb on + strand, within Psest_1028 at 1114.049 kb on + strand, within Psest_1028 at 1114.050 kb on - strand, within Psest_1028 at 1114.050 kb on - strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.105 kb on + strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.106 kb on - strand, within Psest_1028 at 1114.255 kb on + strand at 1114.256 kb on - strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.259 kb on + strand at 1114.260 kb on - strand at 1114.260 kb on - strand at 1114.260 kb on - strand at 1114.260 kb on - strand at 1114.260 kb on - strand at 1114.260 kb on - strand at 1114.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 1,111,416 + Psest_1024 0.82 -0.7 1,111,417 - Psest_1024 0.83 -0.5 1,111,572 - -0.0 1,111,572 - +0.3 1,111,618 - -0.1 1,111,808 + Psest_1025 0.27 +0.9 1,111,808 + Psest_1025 0.27 +0.1 1,111,808 + Psest_1025 0.27 +1.3 1,111,809 - Psest_1025 0.28 -0.3 1,111,983 + Psest_1025 0.50 +0.1 1,111,983 + Psest_1025 0.50 -0.1 1,111,983 + Psest_1025 0.50 +0.3 1,111,983 + Psest_1025 0.50 -0.1 1,111,983 + Psest_1025 0.50 -0.2 1,111,984 - Psest_1025 0.50 -0.8 1,111,984 - Psest_1025 0.50 -0.7 1,111,984 - Psest_1025 0.50 -1.1 1,111,984 - Psest_1025 0.50 -2.4 1,111,987 + Psest_1025 0.51 +1.0 1,112,072 + Psest_1025 0.62 -0.8 1,112,072 + Psest_1025 0.62 -0.1 1,112,072 + Psest_1025 0.62 +0.3 1,112,073 - Psest_1025 0.62 +0.5 1,112,073 - Psest_1025 0.62 +0.5 1,112,073 - Psest_1025 0.62 -0.1 1,112,075 + Psest_1025 0.62 -1.1 1,112,389 + -0.8 1,112,389 + +0.5 1,112,390 - -0.0 1,112,390 - +0.8 1,112,390 - -0.4 1,112,516 + Psest_1026 0.16 +0.5 1,112,516 + Psest_1026 0.16 -0.6 1,112,550 + Psest_1026 0.20 -2.6 1,112,551 - Psest_1026 0.20 +0.3 1,112,657 - Psest_1026 0.32 +0.0 1,112,784 + Psest_1026 0.46 +0.7 1,113,136 + Psest_1026 0.86 +0.2 1,113,193 - -0.7 1,113,202 - -0.5 1,113,203 + +0.0 1,113,203 + -1.5 1,113,204 - +0.8 1,113,204 - -0.4 1,113,274 + +0.4 1,113,274 + +0.2 1,113,275 - +1.1 1,113,275 - -0.2 1,113,694 + Psest_1028 0.29 +1.1 1,113,694 + Psest_1028 0.29 -0.5 1,113,694 + Psest_1028 0.29 -0.5 1,113,695 - Psest_1028 0.29 -1.2 1,113,754 + Psest_1028 0.36 +0.0 1,113,754 + Psest_1028 0.36 +0.2 1,113,754 + Psest_1028 0.36 +0.8 1,113,754 + Psest_1028 0.36 -0.5 1,113,754 + Psest_1028 0.36 +0.0 1,113,754 + Psest_1028 0.36 -0.8 1,113,754 + Psest_1028 0.36 +0.1 1,113,754 + Psest_1028 0.36 -0.8 1,113,754 + Psest_1028 0.36 -0.5 1,113,754 + Psest_1028 0.36 +0.5 1,113,754 + Psest_1028 0.36 -2.1 1,113,754 + Psest_1028 0.36 +0.5 1,113,755 - Psest_1028 0.36 +0.5 1,113,755 - Psest_1028 0.36 -0.2 1,113,755 - Psest_1028 0.36 +0.2 1,113,755 - Psest_1028 0.36 +0.1 1,113,755 - Psest_1028 0.36 -2.6 1,113,755 - Psest_1028 0.36 +0.5 1,113,755 - Psest_1028 0.36 +0.3 1,113,755 - Psest_1028 0.36 -2.2 1,113,930 + Psest_1028 0.58 +0.6 1,113,965 - Psest_1028 0.63 -0.5 1,114,049 + Psest_1028 0.73 -1.5 1,114,049 + Psest_1028 0.73 -1.0 1,114,049 + Psest_1028 0.73 -0.4 1,114,050 - Psest_1028 0.73 -0.0 1,114,050 - Psest_1028 0.73 -0.5 1,114,105 + Psest_1028 0.80 +0.7 1,114,105 + Psest_1028 0.80 +0.1 1,114,105 + Psest_1028 0.80 +0.0 1,114,105 + Psest_1028 0.80 +0.8 1,114,105 + Psest_1028 0.80 +0.3 1,114,105 + Psest_1028 0.80 +0.2 1,114,106 - Psest_1028 0.80 -0.1 1,114,106 - Psest_1028 0.80 -1.4 1,114,106 - Psest_1028 0.80 -0.3 1,114,106 - Psest_1028 0.80 -0.2 1,114,106 - Psest_1028 0.80 -0.7 1,114,106 - Psest_1028 0.80 -0.7 1,114,106 - Psest_1028 0.80 +0.3 1,114,106 - Psest_1028 0.80 +0.2 1,114,106 - Psest_1028 0.80 +0.3 1,114,106 - Psest_1028 0.80 +0.0 1,114,106 - Psest_1028 0.80 -0.3 1,114,255 + +1.1 1,114,256 - +1.0 1,114,259 + +0.8 1,114,259 + -0.7 1,114,259 + -0.7 1,114,259 + +0.2 1,114,259 + -1.1 1,114,259 + +1.8 1,114,259 + -1.0 1,114,259 + -0.5 1,114,259 + +0.2 1,114,259 + -0.2 1,114,259 + -0.4 1,114,259 + +0.2 1,114,259 + +0.5 1,114,260 - -0.2 1,114,260 - -0.1 1,114,260 - +0.0 1,114,260 - -0.7 1,114,260 - -0.3 1,114,260 - +0.1 1,114,260 - -0.0
Or see this region's nucleotide sequence