Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0088

Experiment: RCH2 defined media

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0086 and Psest_0087 are separated by 71 nucleotidesPsest_0087 and Psest_0088 are separated by 273 nucleotidesPsest_0088 and Psest_0089 overlap by 10 nucleotidesPsest_0089 and Psest_0090 are separated by 98 nucleotides Psest_0086: Psest_0086 - hypothetical protein, at 88,546 to 88,971 _0086 Psest_0087: Psest_0087 - hypothetical protein, at 89,043 to 89,285 _0087 Psest_0088: Psest_0088 - phosphonate metabolism protein PhnP, at 89,559 to 90,308 _0088 Psest_0089: Psest_0089 - phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN, at 90,299 to 90,853 _0089 Psest_0090: Psest_0090 - phosphonate metabolism protein PhnM, at 90,952 to 92,097 _0090 Position (kb) 89 90 91Strain fitness (log2 ratio) -1 0 1 2 3at 88.673 kb on - strand, within Psest_0086at 88.769 kb on - strand, within Psest_0086at 88.769 kb on - strand, within Psest_0086at 88.769 kb on - strand, within Psest_0086at 89.112 kb on + strand, within Psest_0087at 89.112 kb on + strand, within Psest_0087at 89.112 kb on + strand, within Psest_0087at 89.113 kb on - strand, within Psest_0087at 89.113 kb on - strand, within Psest_0087at 89.429 kb on - strandat 89.460 kb on - strandat 89.560 kb on + strandat 89.561 kb on - strandat 89.561 kb on - strandat 89.561 kb on - strandat 89.801 kb on + strand, within Psest_0088at 89.802 kb on - strand, within Psest_0088at 89.834 kb on - strand, within Psest_0088at 89.867 kb on - strand, within Psest_0088at 89.983 kb on + strand, within Psest_0088at 89.983 kb on + strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 90.259 kb on + strandat 90.259 kb on + strandat 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.549 kb on + strand, within Psest_0089at 90.621 kb on + strand, within Psest_0089at 90.835 kb on - strandat 90.835 kb on - strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.899 kb on - strandat 90.899 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.182 kb on - strand, within Psest_0090

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined media
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88,673 - Psest_0086 0.30 -0.4
88,769 - Psest_0086 0.52 -0.5
88,769 - Psest_0086 0.52 +1.0
88,769 - Psest_0086 0.52 -1.0
89,112 + Psest_0087 0.28 -1.7
89,112 + Psest_0087 0.28 -0.0
89,112 + Psest_0087 0.28 +0.2
89,113 - Psest_0087 0.29 +0.7
89,113 - Psest_0087 0.29 +0.8
89,429 - -0.4
89,460 - -0.7
89,560 + +0.0
89,561 - +0.6
89,561 - -0.0
89,561 - +0.2
89,801 + Psest_0088 0.32 +1.5
89,802 - Psest_0088 0.32 +0.8
89,834 - Psest_0088 0.37 -0.2
89,867 - Psest_0088 0.41 +0.4
89,983 + Psest_0088 0.57 +2.5
89,983 + Psest_0088 0.57 +0.9
89,984 - Psest_0088 0.57 -0.1
89,984 - Psest_0088 0.57 +0.5
89,984 - Psest_0088 0.57 +0.5
89,984 - Psest_0088 0.57 -0.5
90,259 + +0.5
90,259 + +2.5
90,384 + Psest_0089 0.15 -0.1
90,384 + Psest_0089 0.15 +0.1
90,384 + Psest_0089 0.15 +1.1
90,384 + Psest_0089 0.15 +0.7
90,384 + Psest_0089 0.15 -0.5
90,384 + Psest_0089 0.15 -0.5
90,385 - Psest_0089 0.15 -0.5
90,385 - Psest_0089 0.15 +0.2
90,385 - Psest_0089 0.15 +1.5
90,385 - Psest_0089 0.15 +0.1
90,385 - Psest_0089 0.15 +0.5
90,385 - Psest_0089 0.15 +0.9
90,385 - Psest_0089 0.15 +0.1
90,385 - Psest_0089 0.15 +0.9
90,549 + Psest_0089 0.45 +1.5
90,621 + Psest_0089 0.58 +0.5
90,835 - -0.1
90,835 - -0.1
90,898 + +1.5
90,898 + +1.5
90,898 + -0.5
90,898 + +1.5
90,898 + +0.5
90,898 + -1.1
90,898 + +1.5
90,898 + +1.5
90,899 - -0.1
90,899 - +1.5
91,010 - -1.1
91,010 - +1.5
91,010 - +3.0
91,010 - -0.5
91,010 - -0.1
91,182 - Psest_0090 0.20 +0.5

Or see this region's nucleotide sequence