Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3451 and Psest_3452 are separated by 74 nucleotides Psest_3452 and Psest_3453 are separated by 160 nucleotides
Psest_3451: Psest_3451 - tRNA (uracil-5-)-methyltransferase, at 3,622,598 to 3,623,740
_3451
Psest_3452: Psest_3452 - Zn-dependent hydrolases, including glyoxylases, at 3,623,815 to 3,625,182
_3452
Psest_3453: Psest_3453 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1, at 3,625,343 to 3,626,899
_3453
Position (kb)
3623
3624
3625
3626 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3623.139 kb on - strand, within Psest_3451 at 3623.139 kb on - strand, within Psest_3451 at 3623.292 kb on - strand, within Psest_3451 at 3623.292 kb on - strand, within Psest_3451 at 3623.292 kb on - strand, within Psest_3451 at 3623.511 kb on - strand, within Psest_3451 at 3623.511 kb on - strand, within Psest_3451 at 3623.511 kb on - strand, within Psest_3451 at 3623.511 kb on - strand, within Psest_3451 at 3623.600 kb on + strand, within Psest_3451 at 3623.600 kb on + strand, within Psest_3451 at 3623.600 kb on + strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.601 kb on - strand, within Psest_3451 at 3623.808 kb on + strand at 3623.808 kb on + strand at 3623.809 kb on - strand at 3623.809 kb on - strand at 3623.836 kb on + strand at 3623.836 kb on + strand at 3623.837 kb on - strand at 3623.837 kb on - strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.929 kb on + strand at 3623.930 kb on - strand at 3623.930 kb on - strand at 3623.930 kb on - strand at 3623.930 kb on - strand at 3623.968 kb on + strand, within Psest_3452 at 3623.969 kb on - strand, within Psest_3452 at 3623.969 kb on - strand, within Psest_3452 at 3624.067 kb on + strand, within Psest_3452 at 3624.139 kb on + strand, within Psest_3452 at 3624.140 kb on - strand, within Psest_3452 at 3624.140 kb on - strand, within Psest_3452 at 3624.140 kb on - strand, within Psest_3452 at 3624.305 kb on - strand, within Psest_3452 at 3624.305 kb on - strand, within Psest_3452 at 3624.305 kb on - strand, within Psest_3452 at 3624.305 kb on - strand, within Psest_3452 at 3624.305 kb on - strand, within Psest_3452 at 3624.520 kb on + strand, within Psest_3452 at 3624.521 kb on - strand, within Psest_3452 at 3624.763 kb on + strand, within Psest_3452 at 3624.763 kb on + strand, within Psest_3452 at 3624.764 kb on - strand, within Psest_3452 at 3624.764 kb on - strand, within Psest_3452 at 3624.837 kb on - strand, within Psest_3452 at 3624.844 kb on + strand, within Psest_3452 at 3624.845 kb on - strand, within Psest_3452 at 3625.074 kb on - strand at 3625.347 kb on + strand at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.920 kb on + strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3625.921 kb on - strand, within Psest_3453 at 3626.181 kb on + strand, within Psest_3453
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 3,623,139 - Psest_3451 0.47 +0.4 3,623,139 - Psest_3451 0.47 -1.5 3,623,292 - Psest_3451 0.61 +0.8 3,623,292 - Psest_3451 0.61 +0.7 3,623,292 - Psest_3451 0.61 -0.6 3,623,511 - Psest_3451 0.80 +0.5 3,623,511 - Psest_3451 0.80 +0.1 3,623,511 - Psest_3451 0.80 -0.5 3,623,511 - Psest_3451 0.80 +1.3 3,623,600 + Psest_3451 0.88 +0.7 3,623,600 + Psest_3451 0.88 +0.8 3,623,600 + Psest_3451 0.88 +0.2 3,623,601 - Psest_3451 0.88 +1.8 3,623,601 - Psest_3451 0.88 -0.5 3,623,601 - Psest_3451 0.88 -0.1 3,623,601 - Psest_3451 0.88 +0.0 3,623,601 - Psest_3451 0.88 +0.6 3,623,601 - Psest_3451 0.88 -0.2 3,623,601 - Psest_3451 0.88 +0.3 3,623,601 - Psest_3451 0.88 -0.2 3,623,808 + +2.3 3,623,808 + -1.0 3,623,809 - +1.3 3,623,809 - -0.7 3,623,836 + -0.1 3,623,836 + -1.2 3,623,837 - -1.6 3,623,837 - -1.1 3,623,929 + +1.8 3,623,929 + +0.9 3,623,929 + +1.3 3,623,929 + +0.8 3,623,929 + +0.2 3,623,929 + +1.1 3,623,929 + +0.3 3,623,929 + +0.3 3,623,929 + +0.3 3,623,929 + +0.8 3,623,929 + +0.0 3,623,930 - +0.3 3,623,930 - -0.8 3,623,930 - +1.3 3,623,930 - -0.7 3,623,968 + Psest_3452 0.11 +2.9 3,623,969 - Psest_3452 0.11 +1.3 3,623,969 - Psest_3452 0.11 +0.3 3,624,067 + Psest_3452 0.18 +0.6 3,624,139 + Psest_3452 0.24 -0.7 3,624,140 - Psest_3452 0.24 +0.8 3,624,140 - Psest_3452 0.24 +0.0 3,624,140 - Psest_3452 0.24 +0.8 3,624,305 - Psest_3452 0.36 +0.1 3,624,305 - Psest_3452 0.36 +1.3 3,624,305 - Psest_3452 0.36 +0.3 3,624,305 - Psest_3452 0.36 +0.8 3,624,305 - Psest_3452 0.36 +0.6 3,624,520 + Psest_3452 0.52 -0.2 3,624,521 - Psest_3452 0.52 +0.8 3,624,763 + Psest_3452 0.69 +0.6 3,624,763 + Psest_3452 0.69 -0.7 3,624,764 - Psest_3452 0.69 -0.1 3,624,764 - Psest_3452 0.69 +0.5 3,624,837 - Psest_3452 0.75 +0.8 3,624,844 + Psest_3452 0.75 -0.0 3,624,845 - Psest_3452 0.75 +0.9 3,625,074 - +0.3 3,625,347 + +1.3 3,625,920 + Psest_3453 0.37 +0.3 3,625,920 + Psest_3453 0.37 +1.3 3,625,920 + Psest_3453 0.37 +1.3 3,625,920 + Psest_3453 0.37 +0.0 3,625,920 + Psest_3453 0.37 +1.3 3,625,920 + Psest_3453 0.37 +1.9 3,625,920 + Psest_3453 0.37 +0.3 3,625,921 - Psest_3453 0.37 -0.7 3,625,921 - Psest_3453 0.37 +1.3 3,625,921 - Psest_3453 0.37 +0.9 3,625,921 - Psest_3453 0.37 +1.2 3,625,921 - Psest_3453 0.37 +1.3 3,625,921 - Psest_3453 0.37 -0.2 3,625,921 - Psest_3453 0.37 +1.1 3,626,181 + Psest_3453 0.54 +0.0
Or see this region's nucleotide sequence