Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0256 and Psest_0257 overlap by 8 nucleotides Psest_0257 and Psest_0258 are separated by 229 nucleotides
Psest_0256: Psest_0256 - Predicted Zn-dependent peptidases, at 269,463 to 271,004
_0256
Psest_0257: Psest_0257 - Predicted Zn-dependent peptidases, at 270,997 to 272,349
_0257
Psest_0258: Psest_0258 - signal recognition particle-docking protein FtsY, at 272,579 to 273,859
_0258
Position (kb) 270
271
272
273 Strain fitness (log2 ratio) -1
0
1
2 at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.024 kb on + strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.025 kb on - strand at 271.058 kb on + strand at 271.059 kb on - strand at 271.276 kb on + strand, within Psest_0257 at 271.731 kb on - strand, within Psest_0257 at 271.850 kb on + strand, within Psest_0257 at 272.181 kb on - strand, within Psest_0257 at 272.350 kb on - strand at 272.350 kb on - strand at 272.459 kb on + strand at 272.512 kb on + strand at 272.512 kb on + strand at 272.512 kb on + strand at 272.517 kb on + strand at 272.552 kb on + strand at 272.569 kb on + strand at 272.569 kb on + strand at 272.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 271,024 + +0.1 271,024 + +0.1 271,024 + +0.5 271,024 + -1.1 271,024 + +0.1 271,025 - -0.7 271,025 - +0.3 271,025 - +0.5 271,025 - -0.4 271,025 - +0.1 271,025 - -1.4 271,025 - -1.2 271,025 - -0.4 271,058 + -1.1 271,059 - +0.6 271,276 + Psest_0257 0.21 -0.0 271,731 - Psest_0257 0.54 +0.9 271,850 + Psest_0257 0.63 +2.5 272,181 - Psest_0257 0.88 +1.5 272,350 - -0.2 272,350 - -0.3 272,459 + +0.0 272,512 + +0.7 272,512 + -0.3 272,512 + -0.4 272,517 + -0.1 272,552 + +1.1 272,569 + -0.0 272,569 + +1.4 272,569 + +0.1
Or see this region's nucleotide sequence