Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1012 and Psest_1013 are separated by 74 nucleotides Psest_1013 and Psest_1014 are separated by 199 nucleotides
Psest_1012: Psest_1012 - Signal transduction histidine kinase, at 1,095,694 to 1,098,540
_1012
Psest_1013: Psest_1013 - phosphoribosylamine--glycine ligase, at 1,098,615 to 1,099,907
_1013
Psest_1014: Psest_1014 - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 1,100,107 to 1,101,711
_1014
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1097.706 kb on + strand, within Psest_1012 at 1097.706 kb on + strand, within Psest_1012 at 1097.768 kb on + strand, within Psest_1012 at 1097.768 kb on + strand, within Psest_1012 at 1097.769 kb on - strand, within Psest_1012 at 1097.769 kb on - strand, within Psest_1012 at 1097.769 kb on - strand, within Psest_1012 at 1097.769 kb on - strand, within Psest_1012 at 1097.856 kb on + strand, within Psest_1012 at 1097.897 kb on + strand, within Psest_1012 at 1097.897 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.029 kb on + strand, within Psest_1012 at 1098.158 kb on + strand, within Psest_1012 at 1098.170 kb on + strand, within Psest_1012 at 1098.532 kb on + strand at 1098.535 kb on - strand at 1098.535 kb on - strand at 1098.535 kb on - strand at 1098.575 kb on - strand at 1098.575 kb on - strand at 1098.760 kb on - strand, within Psest_1013 at 1098.878 kb on - strand, within Psest_1013 at 1098.945 kb on + strand, within Psest_1013 at 1098.946 kb on - strand, within Psest_1013 at 1099.099 kb on + strand, within Psest_1013 at 1099.114 kb on + strand, within Psest_1013 at 1099.114 kb on + strand, within Psest_1013 at 1099.115 kb on - strand, within Psest_1013 at 1099.179 kb on - strand, within Psest_1013 at 1099.213 kb on + strand, within Psest_1013 at 1099.214 kb on - strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.547 kb on - strand, within Psest_1013 at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.972 kb on + strand at 1099.979 kb on - strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 1,097,706 + Psest_1012 0.71 -0.6 1,097,706 + Psest_1012 0.71 -0.4 1,097,768 + Psest_1012 0.73 +0.2 1,097,768 + Psest_1012 0.73 -0.8 1,097,769 - Psest_1012 0.73 +0.3 1,097,769 - Psest_1012 0.73 -0.9 1,097,769 - Psest_1012 0.73 -0.9 1,097,769 - Psest_1012 0.73 -1.0 1,097,856 + Psest_1012 0.76 +0.5 1,097,897 + Psest_1012 0.77 -1.1 1,097,897 + Psest_1012 0.77 +0.9 1,098,029 + Psest_1012 0.82 -3.1 1,098,029 + Psest_1012 0.82 +1.0 1,098,029 + Psest_1012 0.82 -0.1 1,098,029 + Psest_1012 0.82 -0.8 1,098,029 + Psest_1012 0.82 +0.2 1,098,029 + Psest_1012 0.82 -0.4 1,098,029 + Psest_1012 0.82 -1.1 1,098,158 + Psest_1012 0.87 +0.2 1,098,170 + Psest_1012 0.87 -2.7 1,098,532 + +0.1 1,098,535 - -0.4 1,098,535 - -0.6 1,098,535 - +2.1 1,098,575 - -0.3 1,098,575 - -0.5 1,098,760 - Psest_1013 0.11 -0.8 1,098,878 - Psest_1013 0.20 -1.7 1,098,945 + Psest_1013 0.26 -1.7 1,098,946 - Psest_1013 0.26 -1.7 1,099,099 + Psest_1013 0.37 -1.1 1,099,114 + Psest_1013 0.39 -1.8 1,099,114 + Psest_1013 0.39 -2.5 1,099,115 - Psest_1013 0.39 -2.3 1,099,179 - Psest_1013 0.44 +0.2 1,099,213 + Psest_1013 0.46 -1.8 1,099,214 - Psest_1013 0.46 -2.0 1,099,510 + Psest_1013 0.69 -2.1 1,099,510 + Psest_1013 0.69 -1.4 1,099,510 + Psest_1013 0.69 -0.3 1,099,510 + Psest_1013 0.69 -1.3 1,099,510 + Psest_1013 0.69 -1.6 1,099,510 + Psest_1013 0.69 -1.3 1,099,547 - Psest_1013 0.72 +0.3 1,099,899 + -0.6 1,099,899 + -1.6 1,099,899 + -3.0 1,099,899 + -1.4 1,099,899 + -1.4 1,099,900 - -2.6 1,099,900 - -2.3 1,099,900 - -2.3 1,099,900 - -2.6 1,099,900 - -0.6 1,099,900 - -1.6 1,099,972 + -1.4 1,099,979 - -1.2 1,100,001 + -4.0 1,100,001 + -0.3 1,100,001 + -1.3 1,100,002 - +2.2 1,100,002 - -0.5 1,100,002 - +0.2 1,100,108 + -1.4
Or see this region's nucleotide sequence