Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0794 and Psest_0795 overlap by 4 nucleotides Psest_0795 and Psest_0796 overlap by 4 nucleotides Psest_0796 and Psest_0797 are separated by 25 nucleotides Psest_0797 and Psest_0798 are separated by 45 nucleotides
Psest_0794: Psest_0794 - parallel beta-helix repeat (two copies), at 869,216 to 870,526
_0794
Psest_0795: Psest_0795 - ABC-type multidrug transport system, ATPase component, at 870,523 to 871,449
_0795
Psest_0796: Psest_0796 - ABC-type transport system involved in multi-copper enzyme maturation, permease component, at 871,446 to 872,276
_0796
Psest_0797: Psest_0797 - Predicted lipoprotein involved in nitrous oxide reduction, at 872,302 to 872,874
_0797
Psest_0798: Psest_0798 - twin arginine-targeting protein translocase, TatA/E family, at 872,920 to 873,102
_0798
Position (kb)
871
872
873 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 870.548 kb on + strand at 870.548 kb on + strand at 870.779 kb on - strand, within Psest_0795 at 870.907 kb on + strand, within Psest_0795 at 870.907 kb on + strand, within Psest_0795 at 870.907 kb on + strand, within Psest_0795 at 870.908 kb on - strand, within Psest_0795 at 870.908 kb on - strand, within Psest_0795 at 870.908 kb on - strand, within Psest_0795 at 871.024 kb on + strand, within Psest_0795 at 871.399 kb on + strand at 871.400 kb on - strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.402 kb on + strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.403 kb on - strand at 871.668 kb on + strand, within Psest_0796 at 871.722 kb on + strand, within Psest_0796 at 871.929 kb on + strand, within Psest_0796 at 871.929 kb on + strand, within Psest_0796 at 871.930 kb on - strand, within Psest_0796 at 871.930 kb on - strand, within Psest_0796 at 871.930 kb on - strand, within Psest_0796 at 872.107 kb on - strand, within Psest_0796 at 872.302 kb on - strand at 872.302 kb on - strand at 872.302 kb on - strand at 872.581 kb on + strand, within Psest_0797 at 872.581 kb on + strand, within Psest_0797 at 872.581 kb on + strand, within Psest_0797 at 872.581 kb on + strand, within Psest_0797 at 872.581 kb on + strand, within Psest_0797 at 872.582 kb on - strand, within Psest_0797 at 872.582 kb on - strand, within Psest_0797 at 872.582 kb on - strand, within Psest_0797 at 872.582 kb on - strand, within Psest_0797 at 872.653 kb on + strand, within Psest_0797 at 872.653 kb on + strand, within Psest_0797 at 872.654 kb on - strand, within Psest_0797 at 872.654 kb on - strand, within Psest_0797 at 872.654 kb on - strand, within Psest_0797 at 872.654 kb on - strand, within Psest_0797 at 872.654 kb on - strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.656 kb on + strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.657 kb on - strand, within Psest_0797 at 872.912 kb on - strand at 872.928 kb on + strand at 873.100 kb on + strand at 873.100 kb on + strand at 873.101 kb on - strand at 873.191 kb on - strand at 873.270 kb on + strand at 873.271 kb on - strand at 873.271 kb on - strand at 873.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 870,548 + +0.0 870,548 + -1.0 870,779 - Psest_0795 0.28 +0.9 870,907 + Psest_0795 0.41 +0.9 870,907 + Psest_0795 0.41 -0.9 870,907 + Psest_0795 0.41 -0.4 870,908 - Psest_0795 0.42 -1.1 870,908 - Psest_0795 0.42 -0.2 870,908 - Psest_0795 0.42 +2.4 871,024 + Psest_0795 0.54 +1.0 871,399 + -1.1 871,400 - +0.8 871,402 + +0.1 871,402 + -0.3 871,402 + +1.1 871,402 + +0.4 871,402 + +0.3 871,402 + +0.7 871,402 + -0.4 871,403 - -0.9 871,403 - -0.0 871,403 - -1.4 871,403 - +0.8 871,403 - +1.5 871,403 - -1.1 871,403 - -0.0 871,403 - -0.3 871,668 + Psest_0796 0.27 -1.7 871,722 + Psest_0796 0.33 -0.2 871,929 + Psest_0796 0.58 -0.2 871,929 + Psest_0796 0.58 +0.3 871,930 - Psest_0796 0.58 -1.3 871,930 - Psest_0796 0.58 +1.0 871,930 - Psest_0796 0.58 -0.2 872,107 - Psest_0796 0.80 -1.5 872,302 - -1.0 872,302 - -1.6 872,302 - +2.4 872,581 + Psest_0797 0.49 +0.3 872,581 + Psest_0797 0.49 +0.3 872,581 + Psest_0797 0.49 -0.2 872,581 + Psest_0797 0.49 -0.4 872,581 + Psest_0797 0.49 -0.0 872,582 - Psest_0797 0.49 +0.3 872,582 - Psest_0797 0.49 -0.5 872,582 - Psest_0797 0.49 +1.4 872,582 - Psest_0797 0.49 -1.9 872,653 + Psest_0797 0.61 +0.8 872,653 + Psest_0797 0.61 -0.2 872,654 - Psest_0797 0.61 -1.2 872,654 - Psest_0797 0.61 -0.3 872,654 - Psest_0797 0.61 +1.8 872,654 - Psest_0797 0.61 -1.1 872,654 - Psest_0797 0.61 -1.1 872,656 + Psest_0797 0.62 +0.6 872,656 + Psest_0797 0.62 -1.2 872,656 + Psest_0797 0.62 -0.5 872,656 + Psest_0797 0.62 -0.0 872,656 + Psest_0797 0.62 -0.4 872,656 + Psest_0797 0.62 +0.4 872,656 + Psest_0797 0.62 -0.5 872,656 + Psest_0797 0.62 -0.3 872,656 + Psest_0797 0.62 -0.4 872,656 + Psest_0797 0.62 +0.5 872,657 - Psest_0797 0.62 -0.9 872,657 - Psest_0797 0.62 -0.2 872,657 - Psest_0797 0.62 +0.1 872,657 - Psest_0797 0.62 +1.8 872,657 - Psest_0797 0.62 -0.2 872,657 - Psest_0797 0.62 -0.7 872,657 - Psest_0797 0.62 +0.0 872,657 - Psest_0797 0.62 -0.4 872,657 - Psest_0797 0.62 +0.4 872,657 - Psest_0797 0.62 +0.4 872,657 - Psest_0797 0.62 +0.8 872,657 - Psest_0797 0.62 -0.9 872,657 - Psest_0797 0.62 +1.8 872,657 - Psest_0797 0.62 +0.0 872,657 - Psest_0797 0.62 +0.9 872,657 - Psest_0797 0.62 -1.2 872,912 - -0.5 872,928 + -1.2 873,100 + +0.0 873,100 + +0.2 873,101 - -0.4 873,191 - -0.8 873,270 + -0.7 873,271 - +0.5 873,271 - -0.5 873,271 - +0.4
Or see this region's nucleotide sequence