Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0477

Experiment: Def Dimethyl Sulfoxide 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0476 and Psest_0477 are separated by 382 nucleotidesPsest_0477 and Psest_0478 are separated by 57 nucleotides Psest_0476: Psest_0476 - type I secretion outer membrane protein, TolC family, at 524,907 to 526,361 _0476 Psest_0477: Psest_0477 - thiamine biosynthesis protein ThiC, at 526,744 to 528,624 _0477 Psest_0478: Psest_0478 - Protein of unknown function (DUF3298)., at 528,682 to 529,419 _0478 Position (kb) 526 527 528 529Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 525.883 kb on - strand, within Psest_0476at 525.922 kb on + strand, within Psest_0476at 525.923 kb on - strand, within Psest_0476at 526.252 kb on + strandat 526.252 kb on + strandat 526.379 kb on + strandat 526.380 kb on - strandat 526.380 kb on - strandat 526.526 kb on + strandat 526.730 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.752 kb on - strandat 526.828 kb on + strandat 526.828 kb on + strandat 526.951 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.280 kb on + strand, within Psest_0477at 527.280 kb on + strand, within Psest_0477at 527.281 kb on - strand, within Psest_0477at 527.701 kb on + strand, within Psest_0477at 527.701 kb on + strand, within Psest_0477at 527.701 kb on + strand, within Psest_0477at 527.701 kb on + strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.702 kb on - strand, within Psest_0477at 527.724 kb on - strand, within Psest_0477at 527.735 kb on - strand, within Psest_0477at 527.872 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.094 kb on + strand, within Psest_0477at 528.095 kb on - strand, within Psest_0477at 528.199 kb on + strand, within Psest_0477at 528.200 kb on - strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.265 kb on + strand, within Psest_0477at 528.266 kb on - strand, within Psest_0477at 528.514 kb on + strandat 528.515 kb on - strandat 528.610 kb on + strandat 528.610 kb on + strandat 528.610 kb on + strandat 528.610 kb on + strandat 528.610 kb on + strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.611 kb on - strandat 528.623 kb on - strandat 528.690 kb on - strandat 528.690 kb on - strandat 528.770 kb on + strand, within Psest_0478at 528.770 kb on + strand, within Psest_0478at 528.770 kb on + strand, within Psest_0478at 528.770 kb on + strand, within Psest_0478at 528.771 kb on - strand, within Psest_0478at 528.771 kb on - strand, within Psest_0478at 528.771 kb on - strand, within Psest_0478at 528.771 kb on - strand, within Psest_0478at 528.771 kb on - strand, within Psest_0478at 528.779 kb on + strand, within Psest_0478at 528.779 kb on + strand, within Psest_0478at 528.779 kb on + strand, within Psest_0478at 528.779 kb on + strand, within Psest_0478at 528.780 kb on - strand, within Psest_0478at 528.780 kb on - strand, within Psest_0478at 528.780 kb on - strand, within Psest_0478at 528.780 kb on - strand, within Psest_0478at 528.983 kb on + strand, within Psest_0478at 528.983 kb on + strand, within Psest_0478at 528.983 kb on + strand, within Psest_0478at 528.984 kb on - strand, within Psest_0478at 528.984 kb on - strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.028 kb on + strand, within Psest_0478at 529.029 kb on - strand, within Psest_0478at 529.029 kb on - strand, within Psest_0478at 529.029 kb on - strand, within Psest_0478at 529.029 kb on - strand, within Psest_0478at 529.029 kb on - strand, within Psest_0478at 529.034 kb on + strand, within Psest_0478at 529.034 kb on + strand, within Psest_0478at 529.034 kb on + strand, within Psest_0478at 529.034 kb on + strand, within Psest_0478at 529.034 kb on + strand, within Psest_0478at 529.035 kb on - strand, within Psest_0478at 529.035 kb on - strand, within Psest_0478at 529.035 kb on - strand, within Psest_0478at 529.035 kb on - strand, within Psest_0478at 529.094 kb on + strand, within Psest_0478at 529.094 kb on + strand, within Psest_0478at 529.095 kb on - strand, within Psest_0478at 529.095 kb on - strand, within Psest_0478at 529.095 kb on - strand, within Psest_0478at 529.139 kb on + strand, within Psest_0478at 529.140 kb on - strand, within Psest_0478at 529.140 kb on - strand, within Psest_0478at 529.140 kb on - strand, within Psest_0478at 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.403 kb on + strandat 529.404 kb on - strandat 529.404 kb on - strandat 529.404 kb on - strandat 529.404 kb on - strandat 529.404 kb on - strandat 529.404 kb on - strandat 529.404 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol%
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525,883 - Psest_0476 0.67 +0.4
525,922 + Psest_0476 0.70 +0.0
525,923 - Psest_0476 0.70 -0.1
526,252 + -0.3
526,252 + -0.3
526,379 + -0.2
526,380 - -1.5
526,380 - -0.6
526,526 + -0.6
526,730 + +2.6
526,751 + -1.4
526,751 + -1.7
526,751 + -2.9
526,751 + -1.8
526,751 + -2.7
526,751 + -0.6
526,751 + -2.5
526,752 - -1.4
526,828 + -0.5
526,828 + -2.1
526,951 + Psest_0477 0.11 -0.1
526,979 + Psest_0477 0.12 -2.5
526,979 + Psest_0477 0.12 -1.5
526,979 + Psest_0477 0.12 -0.9
526,979 + Psest_0477 0.12 -0.6
526,979 + Psest_0477 0.12 -1.6
526,979 + Psest_0477 0.12 -1.3
526,980 - Psest_0477 0.13 -1.5
526,980 - Psest_0477 0.13 -0.4
526,980 - Psest_0477 0.13 -0.4
526,980 - Psest_0477 0.13 -1.7
526,980 - Psest_0477 0.13 -0.6
526,980 - Psest_0477 0.13 -0.3
526,980 - Psest_0477 0.13 -0.9
526,980 - Psest_0477 0.13 -1.6
526,980 - Psest_0477 0.13 -0.8
526,980 - Psest_0477 0.13 -0.5
526,980 - Psest_0477 0.13 -2.3
527,203 + Psest_0477 0.24 -3.2
527,203 + Psest_0477 0.24 -1.3
527,203 + Psest_0477 0.24 -2.1
527,203 + Psest_0477 0.24 -0.4
527,204 - Psest_0477 0.24 -1.5
527,204 - Psest_0477 0.24 -1.8
527,204 - Psest_0477 0.24 -1.4
527,204 - Psest_0477 0.24 -0.6
527,204 - Psest_0477 0.24 -0.9
527,204 - Psest_0477 0.24 -0.6
527,204 - Psest_0477 0.24 -1.3
527,280 + Psest_0477 0.28 -1.6
527,280 + Psest_0477 0.28 -1.3
527,281 - Psest_0477 0.29 -0.3
527,701 + Psest_0477 0.51 -2.4
527,701 + Psest_0477 0.51 -0.1
527,701 + Psest_0477 0.51 -1.5
527,701 + Psest_0477 0.51 -1.7
527,702 - Psest_0477 0.51 -1.7
527,702 - Psest_0477 0.51 -2.0
527,702 - Psest_0477 0.51 -1.3
527,702 - Psest_0477 0.51 -1.6
527,702 - Psest_0477 0.51 -0.8
527,702 - Psest_0477 0.51 -2.5
527,724 - Psest_0477 0.52 +0.1
527,735 - Psest_0477 0.53 -0.5
527,872 + Psest_0477 0.60 -1.0
528,094 + Psest_0477 0.72 -2.2
528,094 + Psest_0477 0.72 -1.6
528,094 + Psest_0477 0.72 +2.9
528,094 + Psest_0477 0.72 -0.9
528,094 + Psest_0477 0.72 -1.1
528,094 + Psest_0477 0.72 -0.0
528,095 - Psest_0477 0.72 -1.5
528,199 + Psest_0477 0.77 -0.8
528,200 - Psest_0477 0.77 -2.9
528,265 + Psest_0477 0.81 -0.5
528,265 + Psest_0477 0.81 -1.8
528,265 + Psest_0477 0.81 -1.8
528,265 + Psest_0477 0.81 -1.0
528,265 + Psest_0477 0.81 -0.6
528,265 + Psest_0477 0.81 -1.6
528,265 + Psest_0477 0.81 -2.3
528,266 - Psest_0477 0.81 -0.9
528,514 + -0.3
528,515 - -0.1
528,610 + -1.7
528,610 + +0.5
528,610 + +0.7
528,610 + +0.2
528,610 + -1.0
528,611 - -0.9
528,611 - +0.3
528,611 - +0.6
528,611 - -0.1
528,611 - +0.3
528,611 - -0.8
528,611 - -0.5
528,611 - -0.8
528,623 - +1.1
528,690 - -0.2
528,690 - +1.0
528,770 + Psest_0478 0.12 -0.8
528,770 + Psest_0478 0.12 -1.0
528,770 + Psest_0478 0.12 -0.6
528,770 + Psest_0478 0.12 +0.5
528,771 - Psest_0478 0.12 +0.7
528,771 - Psest_0478 0.12 +0.2
528,771 - Psest_0478 0.12 +0.1
528,771 - Psest_0478 0.12 +0.3
528,771 - Psest_0478 0.12 +0.3
528,779 + Psest_0478 0.13 +0.3
528,779 + Psest_0478 0.13 -0.4
528,779 + Psest_0478 0.13 -2.7
528,779 + Psest_0478 0.13 -0.2
528,780 - Psest_0478 0.13 +0.3
528,780 - Psest_0478 0.13 -0.8
528,780 - Psest_0478 0.13 -1.3
528,780 - Psest_0478 0.13 +0.4
528,983 + Psest_0478 0.41 -0.9
528,983 + Psest_0478 0.41 -1.5
528,983 + Psest_0478 0.41 +0.3
528,984 - Psest_0478 0.41 -0.6
528,984 - Psest_0478 0.41 -0.5
529,028 + Psest_0478 0.47 -1.3
529,028 + Psest_0478 0.47 -1.1
529,028 + Psest_0478 0.47 +0.3
529,028 + Psest_0478 0.47 +1.9
529,028 + Psest_0478 0.47 +0.2
529,028 + Psest_0478 0.47 -0.5
529,028 + Psest_0478 0.47 +0.7
529,029 - Psest_0478 0.47 -0.9
529,029 - Psest_0478 0.47 -0.9
529,029 - Psest_0478 0.47 +0.9
529,029 - Psest_0478 0.47 -0.7
529,029 - Psest_0478 0.47 +1.0
529,034 + Psest_0478 0.48 -1.3
529,034 + Psest_0478 0.48 -0.1
529,034 + Psest_0478 0.48 -0.0
529,034 + Psest_0478 0.48 -0.3
529,034 + Psest_0478 0.48 -0.2
529,035 - Psest_0478 0.48 -0.7
529,035 - Psest_0478 0.48 -0.9
529,035 - Psest_0478 0.48 -1.0
529,035 - Psest_0478 0.48 -0.8
529,094 + Psest_0478 0.56 +0.2
529,094 + Psest_0478 0.56 +0.2
529,095 - Psest_0478 0.56 +0.6
529,095 - Psest_0478 0.56 -0.8
529,095 - Psest_0478 0.56 +0.1
529,139 + Psest_0478 0.62 -0.9
529,140 - Psest_0478 0.62 +0.3
529,140 - Psest_0478 0.62 -1.8
529,140 - Psest_0478 0.62 -0.5
529,403 + -0.1
529,403 + -0.7
529,403 + +0.5
529,403 + -0.6
529,403 + -0.7
529,403 + +0.9
529,403 + -0.6
529,403 + -0.6
529,403 + +0.7
529,404 - -0.5
529,404 - -0.2
529,404 - -0.7
529,404 - +0.2
529,404 - +0.0
529,404 - -0.4
529,404 - +0.6

Or see this region's nucleotide sequence