Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3960 and Psest_3961 overlap by 4 nucleotides Psest_3961 and Psest_3962 are separated by 117 nucleotides Psest_3962 and Psest_3963 are separated by 169 nucleotides
Psest_3960: Psest_3960 - 3'(2'),5'-bisphosphate nucleotidase, bacterial, at 4,126,860 to 4,127,678
_3960
Psest_3961: Psest_3961 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 4,127,675 to 4,128,241
_3961
Psest_3962: Psest_3962 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED, at 4,128,359 to 4,129,039
_3962
Psest_3963: Psest_3963 - PAS domain S-box, at 4,129,209 to 4,131,770
_3963
Position (kb)
4128
4129
4130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4127.666 kb on - strand at 4127.666 kb on - strand at 4127.721 kb on + strand at 4127.722 kb on - strand at 4127.722 kb on - strand at 4128.024 kb on + strand, within Psest_3961 at 4128.025 kb on - strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.273 kb on + strand at 4128.273 kb on + strand at 4128.284 kb on - strand at 4128.573 kb on - strand, within Psest_3962 at 4128.704 kb on - strand, within Psest_3962 at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.077 kb on + strand at 4129.077 kb on + strand at 4129.077 kb on + strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.176 kb on + strand at 4129.177 kb on - strand at 4129.198 kb on + strand at 4129.199 kb on - strand at 4129.219 kb on + strand at 4129.220 kb on - strand at 4129.220 kb on - strand at 4129.404 kb on + strand at 4129.404 kb on + strand at 4129.404 kb on + strand at 4129.404 kb on + strand at 4129.405 kb on - strand at 4129.540 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.719 kb on + strand, within Psest_3963 at 4129.720 kb on - strand, within Psest_3963 at 4129.720 kb on - strand, within Psest_3963 at 4129.720 kb on - strand, within Psest_3963 at 4129.720 kb on - strand, within Psest_3963 at 4129.829 kb on - strand, within Psest_3963 at 4129.847 kb on - strand, within Psest_3963
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 4,127,666 - +1.1 4,127,666 - -0.6 4,127,721 + +0.8 4,127,722 - +0.6 4,127,722 - -0.3 4,128,024 + Psest_3961 0.62 -0.4 4,128,025 - Psest_3961 0.62 +0.7 4,128,045 + Psest_3961 0.65 +0.2 4,128,045 + Psest_3961 0.65 +0.0 4,128,045 + Psest_3961 0.65 +0.7 4,128,046 - Psest_3961 0.65 +0.0 4,128,046 - Psest_3961 0.65 -0.5 4,128,046 - Psest_3961 0.65 -0.2 4,128,135 + Psest_3961 0.81 +1.2 4,128,135 + Psest_3961 0.81 -0.6 4,128,135 + Psest_3961 0.81 -0.5 4,128,135 + Psest_3961 0.81 -0.8 4,128,135 + Psest_3961 0.81 -0.2 4,128,135 + Psest_3961 0.81 +0.3 4,128,135 + Psest_3961 0.81 -0.9 4,128,135 + Psest_3961 0.81 -0.5 4,128,135 + Psest_3961 0.81 -0.4 4,128,135 + Psest_3961 0.81 -1.7 4,128,136 - Psest_3961 0.81 +0.1 4,128,136 - Psest_3961 0.81 +1.2 4,128,136 - Psest_3961 0.81 -0.1 4,128,136 - Psest_3961 0.81 -0.9 4,128,136 - Psest_3961 0.81 -0.9 4,128,136 - Psest_3961 0.81 -0.4 4,128,136 - Psest_3961 0.81 +0.1 4,128,136 - Psest_3961 0.81 +0.0 4,128,136 - Psest_3961 0.81 -0.5 4,128,136 - Psest_3961 0.81 -0.7 4,128,136 - Psest_3961 0.81 -0.4 4,128,136 - Psest_3961 0.81 +0.3 4,128,221 + +0.4 4,128,221 + -0.8 4,128,221 + +0.2 4,128,221 + +0.1 4,128,221 + -0.4 4,128,222 - +0.5 4,128,222 - -0.6 4,128,222 - -0.1 4,128,222 - -1.3 4,128,222 - +0.1 4,128,222 - -0.4 4,128,222 - -0.9 4,128,222 - -0.4 4,128,222 - -0.6 4,128,273 + -0.9 4,128,273 + -0.1 4,128,284 - -0.3 4,128,573 - Psest_3962 0.31 -2.3 4,128,704 - Psest_3962 0.51 -0.7 4,129,072 + +1.0 4,129,072 + -0.9 4,129,072 + -0.4 4,129,072 + +0.5 4,129,072 + +0.7 4,129,072 + +0.6 4,129,073 - +0.6 4,129,073 - +0.1 4,129,073 - +0.5 4,129,073 - +1.3 4,129,073 - +0.4 4,129,073 - +0.7 4,129,073 - -0.5 4,129,077 + -0.1 4,129,077 + +0.7 4,129,077 + +0.9 4,129,078 - +0.5 4,129,078 - +1.0 4,129,078 - +0.1 4,129,078 - +0.4 4,129,176 + -0.5 4,129,177 - +1.3 4,129,198 + +0.4 4,129,199 - +0.9 4,129,219 + +0.5 4,129,220 - +0.5 4,129,220 - +1.5 4,129,404 + +1.2 4,129,404 + +0.4 4,129,404 + +1.1 4,129,404 + +1.1 4,129,405 - +0.9 4,129,540 + Psest_3963 0.13 +0.8 4,129,719 + Psest_3963 0.20 +0.9 4,129,719 + Psest_3963 0.20 +2.3 4,129,719 + Psest_3963 0.20 +1.3 4,129,719 + Psest_3963 0.20 +0.7 4,129,719 + Psest_3963 0.20 +1.1 4,129,719 + Psest_3963 0.20 +2.2 4,129,720 - Psest_3963 0.20 +0.9 4,129,720 - Psest_3963 0.20 +2.5 4,129,720 - Psest_3963 0.20 +0.9 4,129,720 - Psest_3963 0.20 +0.1 4,129,829 - Psest_3963 0.24 +1.0 4,129,847 - Psest_3963 0.25 -0.2
Or see this region's nucleotide sequence