Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3759 and Psest_3761 are separated by 392 nucleotides Psest_3761 and Psest_3762 are separated by 160 nucleotides Psest_3762 and Psest_3763 are separated by 188 nucleotides Psest_3763 and Psest_3764 are separated by 12 nucleotides
Psest_3759: Psest_3759 - DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog, at 3,929,824 to 3,930,291
_3759
Psest_3761: Psest_3761 - Transcription elongation factor, at 3,930,684 to 3,931,088
_3761
Psest_3762: Psest_3762 - iron donor protein CyaY, at 3,931,249 to 3,931,581
_3762
Psest_3763: Psest_3763 - hypothetical protein, at 3,931,770 to 3,931,910
_3763
Psest_3764: Psest_3764 - diaminopimelate decarboxylase, at 3,931,923 to 3,933,170
_3764
Position (kb)
3931
3932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3930.289 kb on + strand at 3930.290 kb on - strand at 3930.309 kb on + strand at 3930.310 kb on - strand at 3930.331 kb on + strand at 3930.332 kb on - strand at 3930.381 kb on + strand at 3930.381 kb on + strand at 3930.455 kb on + strand at 3930.455 kb on + strand at 3930.455 kb on + strand at 3930.456 kb on - strand at 3930.456 kb on - strand at 3930.456 kb on - strand at 3930.488 kb on + strand at 3930.488 kb on + strand at 3930.489 kb on - strand at 3930.666 kb on + strand at 3930.833 kb on + strand, within Psest_3761 at 3930.833 kb on + strand, within Psest_3761 at 3930.833 kb on + strand, within Psest_3761 at 3930.877 kb on + strand, within Psest_3761 at 3930.877 kb on + strand, within Psest_3761 at 3930.877 kb on + strand, within Psest_3761 at 3931.178 kb on + strand at 3931.179 kb on - strand at 3931.179 kb on - strand at 3931.195 kb on + strand at 3931.250 kb on + strand at 3931.250 kb on + strand at 3931.250 kb on + strand at 3931.516 kb on + strand, within Psest_3762 at 3931.516 kb on + strand, within Psest_3762 at 3931.516 kb on + strand, within Psest_3762 at 3931.516 kb on + strand, within Psest_3762 at 3931.516 kb on + strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.517 kb on - strand, within Psest_3762 at 3931.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 3,930,289 + +0.0 3,930,290 - +0.3 3,930,309 + -0.4 3,930,310 - -0.5 3,930,331 + -0.5 3,930,332 - +0.1 3,930,381 + +0.6 3,930,381 + -0.4 3,930,455 + -0.2 3,930,455 + -0.6 3,930,455 + +0.2 3,930,456 - +0.5 3,930,456 - +0.4 3,930,456 - +0.6 3,930,488 + +0.0 3,930,488 + -1.0 3,930,489 - +1.3 3,930,666 + -0.2 3,930,833 + Psest_3761 0.37 -1.3 3,930,833 + Psest_3761 0.37 +0.8 3,930,833 + Psest_3761 0.37 +0.9 3,930,877 + Psest_3761 0.48 +0.3 3,930,877 + Psest_3761 0.48 +0.6 3,930,877 + Psest_3761 0.48 +0.7 3,931,178 + -1.6 3,931,179 - +0.7 3,931,179 - +1.4 3,931,195 + -0.6 3,931,250 + +2.3 3,931,250 + -0.7 3,931,250 + +0.2 3,931,516 + Psest_3762 0.80 -0.6 3,931,516 + Psest_3762 0.80 -1.4 3,931,516 + Psest_3762 0.80 -1.2 3,931,516 + Psest_3762 0.80 -2.0 3,931,516 + Psest_3762 0.80 -0.4 3,931,517 - Psest_3762 0.80 -0.8 3,931,517 - Psest_3762 0.80 -0.2 3,931,517 - Psest_3762 0.80 -0.1 3,931,517 - Psest_3762 0.80 -2.9 3,931,517 - Psest_3762 0.80 +0.9 3,931,517 - Psest_3762 0.80 -1.7 3,931,708 - +0.1
Or see this region's nucleotide sequence