Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0365 and Psest_0366 are separated by 377 nucleotides Psest_0366 and Psest_0367 overlap by 11 nucleotides Psest_0367 and Psest_0368 are separated by 399 nucleotides
Psest_0365: Psest_0365 - NAD dependent epimerase/dehydratase family., at 391,344 to 392,159
_0365
Psest_0366: Psest_0366 - Predicted membrane protein, at 392,537 to 392,845
_0366
Psest_0367: Psest_0367 - Predicted branched-chain amino acid permease (azaleucine resistance), at 392,835 to 393,551
_0367
Psest_0368: Psest_0368 - Transcriptional regulator, at 393,951 to 394,865
_0368
Position (kb) 392
393
394 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 391.855 kb on + strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.859 kb on + strand, within Psest_0365 at 391.859 kb on + strand, within Psest_0365 at 391.860 kb on - strand, within Psest_0365 at 392.065 kb on + strand, within Psest_0365 at 392.154 kb on - strand at 392.215 kb on - strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.274 kb on + strand at 392.274 kb on + strand at 392.275 kb on - strand at 392.275 kb on - strand at 392.275 kb on - strand at 392.313 kb on + strand at 392.313 kb on + strand at 392.313 kb on + strand at 392.314 kb on - strand at 392.314 kb on - strand at 392.314 kb on - strand at 392.482 kb on - strand at 392.923 kb on + strand, within Psest_0367 at 393.256 kb on + strand, within Psest_0367 at 393.256 kb on + strand, within Psest_0367 at 393.620 kb on + strand at 393.620 kb on + strand at 393.620 kb on + strand at 393.621 kb on - strand at 393.621 kb on - strand at 393.718 kb on + strand at 393.718 kb on + strand at 393.718 kb on + strand at 393.718 kb on + strand at 393.718 kb on + strand at 393.737 kb on - strand at 393.737 kb on - strand at 393.740 kb on + strand at 393.740 kb on + strand at 393.740 kb on + strand at 393.740 kb on + strand at 393.741 kb on - strand at 393.741 kb on - strand at 393.741 kb on - strand at 393.741 kb on - strand at 393.749 kb on + strand at 393.750 kb on - strand at 393.750 kb on - strand at 393.750 kb on - strand at 393.750 kb on - strand at 393.952 kb on + strand at 394.102 kb on - strand, within Psest_0368 at 394.283 kb on - strand, within Psest_0368
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 391,855 + Psest_0365 0.63 -0.4 391,856 - Psest_0365 0.63 +0.1 391,856 - Psest_0365 0.63 -0.7 391,856 - Psest_0365 0.63 -0.1 391,859 + Psest_0365 0.63 +0.9 391,859 + Psest_0365 0.63 +1.3 391,860 - Psest_0365 0.63 +0.2 392,065 + Psest_0365 0.88 -0.7 392,154 - -0.8 392,215 - -0.2 392,272 + -0.1 392,272 + +0.9 392,272 + +0.5 392,272 + +0.7 392,273 - -1.7 392,273 - +0.7 392,273 - -0.7 392,273 - -0.3 392,273 - -0.8 392,273 - -2.0 392,273 - -0.3 392,273 - +2.5 392,273 - +2.9 392,273 - +0.7 392,273 - -1.3 392,273 - -1.9 392,273 - -0.3 392,273 - -1.7 392,273 - +0.1 392,273 - -0.7 392,273 - +0.4 392,273 - +0.5 392,273 - -1.4 392,273 - -1.3 392,274 + +0.2 392,274 + -1.1 392,275 - -0.1 392,275 - +0.5 392,275 - +0.9 392,313 + -0.3 392,313 + -0.7 392,313 + +2.5 392,314 - -0.3 392,314 - +0.1 392,314 - -0.9 392,482 - -0.5 392,923 + Psest_0367 0.12 -1.1 393,256 + Psest_0367 0.59 -0.1 393,256 + Psest_0367 0.59 -0.1 393,620 + +1.9 393,620 + -0.1 393,620 + +0.3 393,621 - -0.3 393,621 - -1.1 393,718 + +2.9 393,718 + +1.9 393,718 + +1.3 393,718 + +1.9 393,718 + +1.9 393,737 - +0.9 393,737 - +0.1 393,740 + +1.9 393,740 + +1.3 393,740 + -0.7 393,740 + -0.4 393,741 - -0.4 393,741 - -0.1 393,741 - -1.3 393,741 - +1.5 393,749 + +2.9 393,750 - +0.3 393,750 - +1.3 393,750 - -0.1 393,750 - +0.1 393,952 + -0.9 394,102 - Psest_0368 0.17 -0.1 394,283 - Psest_0368 0.36 -0.4
Or see this region's nucleotide sequence