Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3006 and Psest_3007 overlap by 1 nucleotides Psest_3007 and Psest_3008 are separated by 2 nucleotides Psest_3008 and Psest_3009 overlap by 4 nucleotides Psest_3009 and Psest_3010 overlap by 8 nucleotides
Psest_3006: Psest_3006 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 3,194,548 to 3,195,597
_3006
Psest_3007: Psest_3007 - Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 3,195,597 to 3,196,115
_3007
Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575
_3008
Psest_3009: Psest_3009 - L-threonine-O-3-phosphate decarboxylase, at 3,197,572 to 3,198,561
_3009
Psest_3010: Psest_3010 - cobalamin biosynthesis protein CobD, at 3,198,554 to 3,199,465
_3010
Position (kb)
3196
3197
3198 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3195.420 kb on + strand, within Psest_3006 at 3195.420 kb on + strand, within Psest_3006 at 3195.420 kb on + strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.425 kb on - strand, within Psest_3006 at 3195.425 kb on - strand, within Psest_3006 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.218 kb on + strand at 3196.218 kb on + strand at 3196.302 kb on + strand, within Psest_3008 at 3196.302 kb on + strand, within Psest_3008 at 3196.303 kb on - strand, within Psest_3008 at 3196.303 kb on - strand, within Psest_3008 at 3196.754 kb on + strand, within Psest_3008 at 3196.754 kb on + strand, within Psest_3008 at 3196.755 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3198.276 kb on - strand, within Psest_3009 at 3198.467 kb on + strand at 3198.518 kb on + strand at 3198.561 kb on + strand at 3198.561 kb on + strand at 3198.562 kb on - strand at 3198.562 kb on - strand at 3198.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 3,195,420 + Psest_3006 0.83 -0.3 3,195,420 + Psest_3006 0.83 +3.7 3,195,420 + Psest_3006 0.83 +1.7 3,195,421 - Psest_3006 0.83 +1.1 3,195,421 - Psest_3006 0.83 +1.7 3,195,421 - Psest_3006 0.83 +1.7 3,195,421 - Psest_3006 0.83 +0.7 3,195,425 - Psest_3006 0.84 -0.2 3,195,425 - Psest_3006 0.84 -0.3 3,196,027 + Psest_3007 0.83 -0.3 3,196,027 + Psest_3007 0.83 -1.2 3,196,027 + Psest_3007 0.83 -0.5 3,196,027 + Psest_3007 0.83 -0.2 3,196,028 - Psest_3007 0.83 -0.1 3,196,028 - Psest_3007 0.83 -0.3 3,196,028 - Psest_3007 0.83 -0.3 3,196,028 - Psest_3007 0.83 +1.7 3,196,028 - Psest_3007 0.83 +1.2 3,196,028 - Psest_3007 0.83 +0.1 3,196,028 - Psest_3007 0.83 +0.1 3,196,028 - Psest_3007 0.83 +1.1 3,196,028 - Psest_3007 0.83 +0.7 3,196,028 - Psest_3007 0.83 -0.6 3,196,028 - Psest_3007 0.83 +0.1 3,196,028 - Psest_3007 0.83 +1.1 3,196,028 - Psest_3007 0.83 +0.7 3,196,028 - Psest_3007 0.83 -0.3 3,196,218 + +0.7 3,196,218 + +3.2 3,196,302 + Psest_3008 0.13 +0.7 3,196,302 + Psest_3008 0.13 +0.2 3,196,303 - Psest_3008 0.13 +1.7 3,196,303 - Psest_3008 0.13 +0.1 3,196,754 + Psest_3008 0.44 +1.7 3,196,754 + Psest_3008 0.44 +0.7 3,196,755 - Psest_3008 0.44 +1.2 3,197,260 - Psest_3008 0.78 -0.5 3,197,260 - Psest_3008 0.78 +0.1 3,198,276 - Psest_3009 0.71 +4.2 3,198,467 + +1.7 3,198,518 + -0.3 3,198,561 + -0.2 3,198,561 + +2.0 3,198,562 - -1.4 3,198,562 - +0.4 3,198,562 - +0.7
Or see this region's nucleotide sequence