Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2330 and Psest_2331 are separated by 261 nucleotides Psest_2331 and Psest_2332 are separated by 30 nucleotides Psest_2332 and Psest_2333 are separated by 363 nucleotides
Psest_2330: Psest_2330 - 2-dehydropantoate 2-reductase, at 2,484,400 to 2,485,356
_2330
Psest_2331: Psest_2331 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 2,485,618 to 2,486,694
_2331
Psest_2332: Psest_2332 - Predicted acetyltransferase, at 2,486,725 to 2,487,018
_2332
Psest_2333: Psest_2333 - Protein of unknown function (DUF3359)., at 2,487,382 to 2,487,663
_2333
Position (kb)
2485
2486
2487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2484.621 kb on + strand, within Psest_2330 at 2484.622 kb on - strand, within Psest_2330 at 2484.622 kb on - strand, within Psest_2330 at 2484.622 kb on - strand, within Psest_2330 at 2484.622 kb on - strand, within Psest_2330 at 2484.622 kb on - strand, within Psest_2330 at 2484.712 kb on - strand, within Psest_2330 at 2484.749 kb on - strand, within Psest_2330 at 2484.991 kb on - strand, within Psest_2330 at 2484.997 kb on + strand, within Psest_2330 at 2484.997 kb on + strand, within Psest_2330 at 2484.997 kb on + strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2484.998 kb on - strand, within Psest_2330 at 2485.001 kb on + strand, within Psest_2330 at 2485.001 kb on + strand, within Psest_2330 at 2485.001 kb on + strand, within Psest_2330 at 2485.002 kb on - strand, within Psest_2330 at 2485.002 kb on - strand, within Psest_2330 at 2485.163 kb on + strand, within Psest_2330 at 2485.298 kb on + strand at 2485.298 kb on + strand at 2485.298 kb on + strand at 2485.299 kb on - strand at 2485.299 kb on - strand at 2485.356 kb on + strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.357 kb on - strand at 2485.488 kb on - strand at 2485.558 kb on + strand at 2485.558 kb on + strand at 2485.558 kb on + strand at 2485.559 kb on - strand at 2485.559 kb on - strand at 2485.559 kb on - strand at 2485.559 kb on - strand at 2485.612 kb on + strand at 2485.613 kb on - strand at 2485.629 kb on - strand at 2485.667 kb on + strand at 2485.869 kb on - strand, within Psest_2331 at 2485.901 kb on - strand, within Psest_2331 at 2485.921 kb on - strand, within Psest_2331 at 2485.954 kb on + strand, within Psest_2331 at 2485.954 kb on + strand, within Psest_2331 at 2485.954 kb on + strand, within Psest_2331 at 2485.954 kb on + strand, within Psest_2331 at 2485.955 kb on - strand, within Psest_2331 at 2485.955 kb on - strand, within Psest_2331 at 2485.957 kb on - strand, within Psest_2331 at 2486.074 kb on + strand, within Psest_2331 at 2486.075 kb on - strand, within Psest_2331 at 2486.075 kb on - strand, within Psest_2331 at 2486.075 kb on - strand, within Psest_2331 at 2486.303 kb on - strand, within Psest_2331 at 2486.303 kb on - strand, within Psest_2331 at 2486.340 kb on + strand, within Psest_2331 at 2486.340 kb on + strand, within Psest_2331 at 2486.341 kb on - strand, within Psest_2331 at 2486.344 kb on + strand, within Psest_2331 at 2486.345 kb on - strand, within Psest_2331 at 2486.345 kb on - strand, within Psest_2331 at 2486.345 kb on - strand, within Psest_2331 at 2486.345 kb on - strand, within Psest_2331 at 2486.345 kb on - strand, within Psest_2331 at 2486.584 kb on + strand, within Psest_2331 at 2486.584 kb on + strand, within Psest_2331 at 2486.584 kb on + strand, within Psest_2331 at 2486.584 kb on + strand, within Psest_2331 at 2486.726 kb on + strand at 2486.726 kb on + strand at 2486.775 kb on - strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.786 kb on + strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.787 kb on - strand, within Psest_2332 at 2486.811 kb on - strand, within Psest_2332 at 2486.888 kb on - strand, within Psest_2332 at 2486.896 kb on - strand, within Psest_2332 at 2486.900 kb on + strand, within Psest_2332 at 2486.900 kb on + strand, within Psest_2332 at 2486.901 kb on - strand, within Psest_2332 at 2486.901 kb on - strand, within Psest_2332 at 2486.905 kb on - strand, within Psest_2332 at 2486.905 kb on - strand, within Psest_2332 at 2486.905 kb on - strand, within Psest_2332 at 2486.905 kb on - strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.933 kb on + strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.934 kb on - strand, within Psest_2332 at 2486.942 kb on + strand, within Psest_2332 at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.080 kb on + strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.081 kb on - strand at 2487.083 kb on - strand at 2487.099 kb on + strand at 2487.099 kb on + strand at 2487.099 kb on + strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.100 kb on - strand at 2487.117 kb on - strand at 2487.157 kb on + strand at 2487.157 kb on + strand at 2487.157 kb on + strand at 2487.157 kb on + strand at 2487.158 kb on - strand at 2487.158 kb on - strand at 2487.209 kb on + strand at 2487.209 kb on + strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.210 kb on - strand at 2487.217 kb on + strand at 2487.217 kb on + strand at 2487.217 kb on + strand at 2487.217 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.221 kb on + strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.222 kb on - strand at 2487.270 kb on + strand at 2487.271 kb on - strand at 2487.300 kb on + strand at 2487.323 kb on - strand at 2487.323 kb on - strand at 2487.326 kb on + strand at 2487.327 kb on - strand at 2487.327 kb on - strand at 2487.331 kb on + strand at 2487.331 kb on + strand at 2487.331 kb on + strand at 2487.331 kb on + strand at 2487.331 kb on + strand at 2487.336 kb on + strand at 2487.336 kb on + strand at 2487.336 kb on + strand at 2487.336 kb on + strand at 2487.336 kb on + strand at 2487.337 kb on - strand at 2487.338 kb on + strand at 2487.339 kb on - strand at 2487.339 kb on - strand at 2487.339 kb on - strand at 2487.339 kb on - strand at 2487.339 kb on - strand at 2487.342 kb on + strand at 2487.342 kb on + strand at 2487.342 kb on + strand at 2487.342 kb on + strand at 2487.342 kb on + strand at 2487.343 kb on - strand at 2487.343 kb on - strand at 2487.343 kb on - strand at 2487.343 kb on - strand at 2487.343 kb on - strand at 2487.343 kb on - strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.345 kb on + strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.346 kb on - strand at 2487.382 kb on - strand at 2487.557 kb on - strand, within Psest_2333 at 2487.661 kb on + strand at 2487.661 kb on + strand at 2487.661 kb on + strand at 2487.661 kb on + strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.662 kb on - strand at 2487.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 2,484,621 + Psest_2330 0.23 -1.0 2,484,622 - Psest_2330 0.23 +0.2 2,484,622 - Psest_2330 0.23 +0.7 2,484,622 - Psest_2330 0.23 +1.5 2,484,622 - Psest_2330 0.23 +0.5 2,484,622 - Psest_2330 0.23 +1.2 2,484,712 - Psest_2330 0.33 -0.4 2,484,749 - Psest_2330 0.36 +0.3 2,484,991 - Psest_2330 0.62 -0.3 2,484,997 + Psest_2330 0.62 +0.2 2,484,997 + Psest_2330 0.62 -1.3 2,484,997 + Psest_2330 0.62 +0.3 2,484,998 - Psest_2330 0.62 +0.2 2,484,998 - Psest_2330 0.62 -0.8 2,484,998 - Psest_2330 0.62 -0.1 2,484,998 - Psest_2330 0.62 +0.6 2,484,998 - Psest_2330 0.62 -0.9 2,484,998 - Psest_2330 0.62 -0.9 2,484,998 - Psest_2330 0.62 -0.2 2,484,998 - Psest_2330 0.62 -0.3 2,484,998 - Psest_2330 0.62 +0.4 2,484,998 - Psest_2330 0.62 -2.5 2,485,001 + Psest_2330 0.63 +0.8 2,485,001 + Psest_2330 0.63 +0.2 2,485,001 + Psest_2330 0.63 +0.3 2,485,002 - Psest_2330 0.63 +0.6 2,485,002 - Psest_2330 0.63 +0.3 2,485,163 + Psest_2330 0.80 -0.2 2,485,298 + -0.2 2,485,298 + +0.3 2,485,298 + +0.3 2,485,299 - +1.3 2,485,299 - +0.9 2,485,356 + -0.8 2,485,357 - -0.2 2,485,357 - -0.3 2,485,357 - -1.8 2,485,357 - +0.4 2,485,357 - +0.2 2,485,357 - -0.2 2,485,357 - -0.7 2,485,357 - +0.2 2,485,357 - +0.1 2,485,357 - -0.6 2,485,357 - -0.0 2,485,488 - +0.5 2,485,558 + -0.1 2,485,558 + -0.0 2,485,558 + -0.1 2,485,559 - -1.3 2,485,559 - -1.3 2,485,559 - -0.1 2,485,559 - +0.8 2,485,612 + +0.4 2,485,613 - -1.8 2,485,629 - -1.3 2,485,667 + -0.9 2,485,869 - Psest_2331 0.23 -0.2 2,485,901 - Psest_2331 0.26 -1.3 2,485,921 - Psest_2331 0.28 -1.0 2,485,954 + Psest_2331 0.31 -1.0 2,485,954 + Psest_2331 0.31 -0.8 2,485,954 + Psest_2331 0.31 -0.8 2,485,954 + Psest_2331 0.31 -0.9 2,485,955 - Psest_2331 0.31 -0.2 2,485,955 - Psest_2331 0.31 -1.5 2,485,957 - Psest_2331 0.31 -3.1 2,486,074 + Psest_2331 0.42 -0.3 2,486,075 - Psest_2331 0.42 -1.5 2,486,075 - Psest_2331 0.42 -1.8 2,486,075 - Psest_2331 0.42 -1.1 2,486,303 - Psest_2331 0.64 -1.5 2,486,303 - Psest_2331 0.64 -1.5 2,486,340 + Psest_2331 0.67 -1.5 2,486,340 + Psest_2331 0.67 -1.2 2,486,341 - Psest_2331 0.67 -1.1 2,486,344 + Psest_2331 0.67 -0.5 2,486,345 - Psest_2331 0.68 +0.7 2,486,345 - Psest_2331 0.68 -0.6 2,486,345 - Psest_2331 0.68 -0.7 2,486,345 - Psest_2331 0.68 -0.6 2,486,345 - Psest_2331 0.68 -0.6 2,486,584 + Psest_2331 0.90 -0.7 2,486,584 + Psest_2331 0.90 -0.5 2,486,584 + Psest_2331 0.90 -1.4 2,486,584 + Psest_2331 0.90 -1.1 2,486,726 + +0.1 2,486,726 + +0.7 2,486,775 - Psest_2332 0.17 -0.4 2,486,786 + Psest_2332 0.21 -0.4 2,486,786 + Psest_2332 0.21 -0.2 2,486,786 + Psest_2332 0.21 +0.7 2,486,786 + Psest_2332 0.21 +0.1 2,486,786 + Psest_2332 0.21 +0.1 2,486,786 + Psest_2332 0.21 -1.6 2,486,786 + Psest_2332 0.21 +0.6 2,486,786 + Psest_2332 0.21 -1.0 2,486,787 - Psest_2332 0.21 +0.1 2,486,787 - Psest_2332 0.21 +0.1 2,486,787 - Psest_2332 0.21 +0.1 2,486,787 - Psest_2332 0.21 -0.0 2,486,787 - Psest_2332 0.21 +0.1 2,486,787 - Psest_2332 0.21 +0.1 2,486,787 - Psest_2332 0.21 -0.4 2,486,787 - Psest_2332 0.21 +0.5 2,486,811 - Psest_2332 0.29 +0.5 2,486,888 - Psest_2332 0.55 -0.1 2,486,896 - Psest_2332 0.58 -0.2 2,486,900 + Psest_2332 0.60 -0.8 2,486,900 + Psest_2332 0.60 +0.2 2,486,901 - Psest_2332 0.60 +0.4 2,486,901 - Psest_2332 0.60 -1.4 2,486,905 - Psest_2332 0.61 +0.6 2,486,905 - Psest_2332 0.61 -0.4 2,486,905 - Psest_2332 0.61 -0.2 2,486,905 - Psest_2332 0.61 +0.7 2,486,933 + Psest_2332 0.71 -0.7 2,486,933 + Psest_2332 0.71 -0.2 2,486,933 + Psest_2332 0.71 +1.3 2,486,933 + Psest_2332 0.71 -0.4 2,486,933 + Psest_2332 0.71 -0.2 2,486,933 + Psest_2332 0.71 +0.8 2,486,934 - Psest_2332 0.71 +0.2 2,486,934 - Psest_2332 0.71 -0.0 2,486,934 - Psest_2332 0.71 +0.1 2,486,934 - Psest_2332 0.71 +0.2 2,486,934 - Psest_2332 0.71 -0.8 2,486,934 - Psest_2332 0.71 +0.0 2,486,934 - Psest_2332 0.71 +0.4 2,486,934 - Psest_2332 0.71 +0.0 2,486,942 + Psest_2332 0.74 -0.2 2,487,080 + -0.5 2,487,080 + -0.5 2,487,080 + -0.1 2,487,080 + -0.2 2,487,080 + +1.1 2,487,080 + +0.2 2,487,080 + +0.1 2,487,080 + -0.1 2,487,080 + -0.6 2,487,080 + -0.2 2,487,081 - -1.3 2,487,081 - +0.4 2,487,081 - +0.1 2,487,081 - +0.8 2,487,081 - -0.0 2,487,081 - -0.2 2,487,081 - -0.2 2,487,081 - +1.8 2,487,081 - -0.0 2,487,081 - -0.2 2,487,081 - -0.8 2,487,081 - -0.5 2,487,081 - -0.7 2,487,081 - -1.3 2,487,081 - -0.2 2,487,081 - +0.2 2,487,081 - -0.6 2,487,081 - -0.4 2,487,081 - -0.3 2,487,081 - +0.3 2,487,081 - -0.8 2,487,081 - +0.7 2,487,081 - -1.2 2,487,081 - +0.4 2,487,081 - -0.1 2,487,083 - -0.2 2,487,099 + +0.3 2,487,099 + -0.7 2,487,099 + -0.1 2,487,100 - -0.1 2,487,100 - -0.3 2,487,100 - -0.6 2,487,100 - +0.0 2,487,100 - -0.3 2,487,100 - +0.1 2,487,100 - -0.1 2,487,100 - -0.3 2,487,117 - -0.4 2,487,157 + +0.4 2,487,157 + +0.0 2,487,157 + -0.1 2,487,157 + +0.5 2,487,158 - -0.3 2,487,158 - -0.3 2,487,209 + +0.2 2,487,209 + +0.2 2,487,210 - -0.7 2,487,210 - -0.5 2,487,210 - -0.0 2,487,210 - -0.2 2,487,210 - -0.7 2,487,210 - -0.3 2,487,210 - -0.0 2,487,210 - -0.6 2,487,210 - -0.5 2,487,210 - +0.2 2,487,217 + +2.1 2,487,217 + +0.4 2,487,217 + +0.7 2,487,217 + -0.2 2,487,221 + -1.8 2,487,221 + +0.1 2,487,221 + +0.3 2,487,221 + +0.4 2,487,221 + -0.4 2,487,221 + +0.1 2,487,221 + +0.6 2,487,222 - +0.2 2,487,222 - -0.0 2,487,222 - -1.2 2,487,222 - -0.1 2,487,222 - -0.1 2,487,222 - +0.8 2,487,222 - -0.1 2,487,222 - +0.3 2,487,222 - -1.7 2,487,222 - +0.0 2,487,270 + -0.0 2,487,271 - -1.0 2,487,300 + +0.5 2,487,323 - -1.5 2,487,323 - -0.4 2,487,326 + +1.0 2,487,327 - +0.0 2,487,327 - +0.2 2,487,331 + -0.6 2,487,331 + +0.7 2,487,331 + -0.3 2,487,331 + -0.0 2,487,331 + +0.3 2,487,336 + -0.0 2,487,336 + -0.1 2,487,336 + -0.3 2,487,336 + -0.6 2,487,336 + -0.9 2,487,337 - -0.6 2,487,338 + -0.0 2,487,339 - -0.6 2,487,339 - -0.3 2,487,339 - -0.9 2,487,339 - -0.3 2,487,339 - +0.9 2,487,342 + +0.5 2,487,342 + +0.2 2,487,342 + +0.1 2,487,342 + +0.3 2,487,342 + +0.5 2,487,343 - -0.9 2,487,343 - -0.8 2,487,343 - +0.7 2,487,343 - +0.3 2,487,343 - +0.1 2,487,343 - -0.3 2,487,345 + -0.2 2,487,345 + +1.0 2,487,345 + -0.3 2,487,345 + -0.1 2,487,345 + -2.9 2,487,345 + -0.5 2,487,345 + +0.3 2,487,346 - -0.4 2,487,346 - -0.2 2,487,346 - -0.4 2,487,346 - -0.4 2,487,346 - +0.6 2,487,346 - -0.7 2,487,346 - -0.2 2,487,346 - +0.2 2,487,346 - -0.3 2,487,346 - +0.4 2,487,346 - -0.1 2,487,382 - -0.6 2,487,557 - Psest_2333 0.62 -0.2 2,487,661 + -0.5 2,487,661 + +1.1 2,487,661 + -0.6 2,487,661 + +0.0 2,487,662 - +0.1 2,487,662 - +0.2 2,487,662 - -0.4 2,487,662 - +0.3 2,487,662 - +0.9 2,487,662 - -0.3 2,487,662 - +1.1 2,487,662 - -0.0 2,487,662 - -0.3 2,487,662 - +0.1 2,487,662 - +0.0 2,487,662 - -0.6 2,487,662 - +0.1 2,487,670 - +1.2
Or see this region's nucleotide sequence