Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1649 and Psest_1650 are separated by 186 nucleotides Psest_1650 and Psest_1651 are separated by 142 nucleotides
Psest_1649: Psest_1649 - Predicted exonuclease of the beta-lactamase fold involved in RNA processing, at 1,754,648 to 1,755,661
_1649
Psest_1650: Psest_1650 - hypothetical protein, at 1,755,848 to 1,756,417
_1650
Psest_1651: Psest_1651 - Superfamily II DNA/RNA helicases, SNF2 family, at 1,756,560 to 1,758,890
_1651
Position (kb)
1755
1756
1757 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1754.913 kb on + strand, within Psest_1649 at 1755.015 kb on + strand, within Psest_1649 at 1755.015 kb on + strand, within Psest_1649 at 1755.016 kb on - strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.211 kb on + strand, within Psest_1649 at 1755.215 kb on + strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.241 kb on - strand, within Psest_1649 at 1755.330 kb on + strand, within Psest_1649 at 1755.330 kb on + strand, within Psest_1649 at 1755.331 kb on - strand, within Psest_1649 at 1755.363 kb on + strand, within Psest_1649 at 1755.363 kb on + strand, within Psest_1649 at 1755.364 kb on - strand, within Psest_1649 at 1755.450 kb on + strand, within Psest_1649 at 1755.526 kb on - strand, within Psest_1649 at 1755.606 kb on + strand at 1755.606 kb on + strand at 1755.627 kb on + strand at 1755.627 kb on + strand at 1755.665 kb on + strand at 1755.666 kb on - strand at 1755.686 kb on + strand at 1755.687 kb on - strand at 1755.687 kb on - strand at 1755.687 kb on - strand at 1755.785 kb on + strand at 1755.785 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.208 kb on + strand, within Psest_1650 at 1756.208 kb on + strand, within Psest_1650 at 1756.939 kb on + strand, within Psest_1651 at 1756.939 kb on + strand, within Psest_1651 at 1756.939 kb on + strand, within Psest_1651 at 1756.940 kb on - strand, within Psest_1651 at 1756.940 kb on - strand, within Psest_1651 at 1757.410 kb on + strand, within Psest_1651
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 1,754,913 + Psest_1649 0.26 +2.2 1,755,015 + Psest_1649 0.36 -0.2 1,755,015 + Psest_1649 0.36 -0.2 1,755,016 - Psest_1649 0.36 -0.8 1,755,033 + Psest_1649 0.38 +0.6 1,755,033 + Psest_1649 0.38 -0.4 1,755,033 + Psest_1649 0.38 +0.8 1,755,033 + Psest_1649 0.38 -1.8 1,755,033 + Psest_1649 0.38 +0.4 1,755,033 + Psest_1649 0.38 +1.2 1,755,033 + Psest_1649 0.38 +0.2 1,755,033 + Psest_1649 0.38 +1.6 1,755,033 + Psest_1649 0.38 +0.1 1,755,034 - Psest_1649 0.38 +0.5 1,755,034 - Psest_1649 0.38 -0.6 1,755,034 - Psest_1649 0.38 -0.4 1,755,034 - Psest_1649 0.38 +0.1 1,755,211 + Psest_1649 0.56 +0.5 1,755,215 + Psest_1649 0.56 -0.5 1,755,216 - Psest_1649 0.56 -0.3 1,755,216 - Psest_1649 0.56 +0.1 1,755,216 - Psest_1649 0.56 +0.2 1,755,240 + Psest_1649 0.58 -0.1 1,755,240 + Psest_1649 0.58 -0.1 1,755,240 + Psest_1649 0.58 -0.7 1,755,241 - Psest_1649 0.58 -0.8 1,755,330 + Psest_1649 0.67 -1.2 1,755,330 + Psest_1649 0.67 -2.3 1,755,331 - Psest_1649 0.67 -0.6 1,755,363 + Psest_1649 0.71 -0.3 1,755,363 + Psest_1649 0.71 -0.7 1,755,364 - Psest_1649 0.71 -0.7 1,755,450 + Psest_1649 0.79 +1.0 1,755,526 - Psest_1649 0.87 -0.8 1,755,606 + -2.1 1,755,606 + +0.2 1,755,627 + +0.4 1,755,627 + +1.6 1,755,665 + -0.7 1,755,666 - +0.3 1,755,686 + -0.4 1,755,687 - -1.7 1,755,687 - -0.3 1,755,687 - -0.5 1,755,785 + -0.2 1,755,785 + -0.2 1,755,787 + -0.1 1,755,787 + +0.2 1,755,787 + +0.1 1,755,787 + +1.6 1,755,787 + +0.1 1,755,787 + -0.7 1,755,787 + -1.4 1,755,788 - +0.2 1,755,788 - -2.1 1,755,788 - +0.5 1,755,788 - +0.7 1,755,788 - +1.3 1,755,788 - +1.6 1,755,788 - +0.1 1,756,056 + Psest_1650 0.36 +0.1 1,756,056 + Psest_1650 0.36 -0.4 1,756,056 + Psest_1650 0.36 -0.3 1,756,056 + Psest_1650 0.36 +0.2 1,756,056 + Psest_1650 0.36 +0.6 1,756,056 + Psest_1650 0.36 -0.7 1,756,056 + Psest_1650 0.36 -0.1 1,756,057 - Psest_1650 0.37 -0.4 1,756,057 - Psest_1650 0.37 -0.6 1,756,057 - Psest_1650 0.37 -1.4 1,756,057 - Psest_1650 0.37 -0.5 1,756,057 - Psest_1650 0.37 -1.7 1,756,057 - Psest_1650 0.37 -1.2 1,756,057 - Psest_1650 0.37 -0.9 1,756,057 - Psest_1650 0.37 +0.3 1,756,057 - Psest_1650 0.37 -1.7 1,756,057 - Psest_1650 0.37 -1.9 1,756,208 + Psest_1650 0.63 +0.1 1,756,208 + Psest_1650 0.63 -1.2 1,756,939 + Psest_1651 0.16 +1.4 1,756,939 + Psest_1651 0.16 -0.0 1,756,939 + Psest_1651 0.16 +0.2 1,756,940 - Psest_1651 0.16 +0.9 1,756,940 - Psest_1651 0.16 +0.0 1,757,410 + Psest_1651 0.36 +0.0
Or see this region's nucleotide sequence