Experiment: Def Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1519 and Psest_1520 are separated by 70 nucleotides Psest_1520 and Psest_1521 are separated by 56 nucleotides Psest_1521 and Psest_1522 are separated by 8 nucleotides Psest_1522 and Psest_1523 are separated by 37 nucleotides
Psest_1519: Psest_1519 - DNA polymerase elongation subunit (family B), at 1,617,390 to 1,619,765
_1519
Psest_1520: Psest_1520 - hypothetical protein, at 1,619,836 to 1,620,144
_1520
Psest_1521: Psest_1521 - Uncharacterized protein conserved in bacteria, at 1,620,201 to 1,620,545
_1521
Psest_1522: Psest_1522 - hypothetical protein, at 1,620,554 to 1,620,745
_1522
Psest_1523: Psest_1523 - Dicarboxylate transport., at 1,620,783 to 1,623,344
_1523
Position (kb)
1620
1621 Strain fitness (log2 ratio)
-1
0
1 at 1619.578 kb on - strand at 1619.601 kb on + strand at 1619.601 kb on + strand at 1619.601 kb on + strand at 1619.667 kb on + strand at 1619.667 kb on + strand at 1619.668 kb on - strand at 1619.668 kb on - strand at 1619.668 kb on - strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.836 kb on - strand at 1619.836 kb on - strand at 1619.836 kb on - strand at 1619.866 kb on + strand at 1619.867 kb on - strand, within Psest_1520 at 1619.908 kb on - strand, within Psest_1520 at 1619.953 kb on + strand, within Psest_1520 at 1619.953 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.026 kb on - strand, within Psest_1520 at 1620.070 kb on + strand, within Psest_1520 at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.143 kb on - strand at 1620.143 kb on - strand at 1620.378 kb on - strand, within Psest_1521 at 1620.535 kb on + strand at 1620.536 kb on - strand at 1620.536 kb on - strand at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.689 kb on - strand, within Psest_1522 at 1620.837 kb on + strand at 1620.838 kb on - strand at 1620.939 kb on + strand at 1620.970 kb on + strand at 1621.448 kb on + strand, within Psest_1523 at 1621.522 kb on + strand, within Psest_1523 at 1621.523 kb on - strand, within Psest_1523 at 1621.523 kb on - strand, within Psest_1523 at 1621.523 kb on - strand, within Psest_1523 at 1621.523 kb on - strand, within Psest_1523 at 1621.710 kb on + strand, within Psest_1523 at 1621.710 kb on + strand, within Psest_1523 at 1621.710 kb on + strand, within Psest_1523 at 1621.710 kb on + strand, within Psest_1523 at 1621.710 kb on + strand, within Psest_1523 at 1621.711 kb on - strand, within Psest_1523 at 1621.711 kb on - strand, within Psest_1523 at 1621.711 kb on - strand, within Psest_1523 at 1621.711 kb on - strand, within Psest_1523 at 1621.711 kb on - strand, within Psest_1523 at 1621.716 kb on + strand, within Psest_1523 at 1621.716 kb on + strand, within Psest_1523 at 1621.717 kb on - strand, within Psest_1523 at 1621.717 kb on - strand, within Psest_1523 at 1621.717 kb on - strand, within Psest_1523
Per-strain Table
Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol% remove 1,619,578 - +0.7 1,619,601 + +0.3 1,619,601 + +0.2 1,619,601 + -0.5 1,619,667 + +0.2 1,619,667 + -0.7 1,619,668 - -0.1 1,619,668 - +0.9 1,619,668 - +0.2 1,619,835 + +0.2 1,619,835 + -0.1 1,619,835 + +1.1 1,619,835 + +1.3 1,619,835 + +0.8 1,619,835 + -0.2 1,619,835 + +0.7 1,619,836 - -1.5 1,619,836 - -0.5 1,619,836 - +0.3 1,619,866 + +0.3 1,619,867 - Psest_1520 0.10 +0.7 1,619,908 - Psest_1520 0.23 -0.3 1,619,953 + Psest_1520 0.38 +0.2 1,619,953 + Psest_1520 0.38 -0.6 1,620,025 + Psest_1520 0.61 -0.1 1,620,025 + Psest_1520 0.61 -1.4 1,620,025 + Psest_1520 0.61 +1.1 1,620,026 - Psest_1520 0.61 -1.1 1,620,070 + Psest_1520 0.76 -0.3 1,620,142 + +0.4 1,620,142 + +0.5 1,620,142 + +0.7 1,620,142 + -0.3 1,620,143 - -0.3 1,620,143 - -0.9 1,620,378 - Psest_1521 0.51 +0.6 1,620,535 + -1.1 1,620,536 - +0.0 1,620,536 - -0.2 1,620,612 + Psest_1522 0.30 +0.4 1,620,612 + Psest_1522 0.30 +0.4 1,620,612 + Psest_1522 0.30 -0.4 1,620,612 + Psest_1522 0.30 -0.8 1,620,689 - Psest_1522 0.70 -0.6 1,620,837 + -0.6 1,620,838 - +0.7 1,620,939 + -1.4 1,620,970 + -0.7 1,621,448 + Psest_1523 0.26 +0.1 1,621,522 + Psest_1523 0.29 +0.7 1,621,523 - Psest_1523 0.29 +0.0 1,621,523 - Psest_1523 0.29 -0.2 1,621,523 - Psest_1523 0.29 -0.4 1,621,523 - Psest_1523 0.29 +0.7 1,621,710 + Psest_1523 0.36 +0.4 1,621,710 + Psest_1523 0.36 -0.6 1,621,710 + Psest_1523 0.36 -0.7 1,621,710 + Psest_1523 0.36 -0.4 1,621,710 + Psest_1523 0.36 -0.4 1,621,711 - Psest_1523 0.36 -0.6 1,621,711 - Psest_1523 0.36 -0.6 1,621,711 - Psest_1523 0.36 +0.4 1,621,711 - Psest_1523 0.36 +0.8 1,621,711 - Psest_1523 0.36 +0.0 1,621,716 + Psest_1523 0.36 -0.4 1,621,716 + Psest_1523 0.36 +0.0 1,621,717 - Psest_1523 0.36 -0.9 1,621,717 - Psest_1523 0.36 -0.6 1,621,717 - Psest_1523 0.36 -0.2
Or see this region's nucleotide sequence