Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1518

Experiment: Def Dimethyl Sulfoxide 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1517 and Psest_1518 are separated by 132 nucleotidesPsest_1518 and Psest_1519 are separated by 166 nucleotides Psest_1517: Psest_1517 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,616,132 to 1,617,001 _1517 Psest_1518: Psest_1518 - tRNA_Ser_CGA, at 1,617,134 to 1,617,223 _1518 Psest_1519: Psest_1519 - DNA polymerase elongation subunit (family B), at 1,617,390 to 1,619,765 _1519 Position (kb) 1617 1618Strain fitness (log2 ratio) -2 -1 0 1at 1616.983 kb on + strandat 1616.983 kb on + strandat 1617.118 kb on + strandat 1617.181 kb on - strand, within Psest_1518at 1617.196 kb on + strand, within Psest_1518at 1617.196 kb on + strand, within Psest_1518at 1617.197 kb on - strand, within Psest_1518at 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.225 kb on + strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.226 kb on - strandat 1617.231 kb on + strandat 1617.232 kb on - strandat 1617.232 kb on - strandat 1617.232 kb on - strandat 1617.427 kb on + strandat 1617.428 kb on - strandat 1617.449 kb on + strandat 1617.652 kb on - strand, within Psest_1519at 1617.652 kb on - strand, within Psest_1519at 1617.903 kb on + strand, within Psest_1519at 1617.903 kb on + strand, within Psest_1519at 1617.903 kb on + strand, within Psest_1519at 1617.904 kb on - strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.942 kb on + strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.943 kb on - strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.944 kb on + strand, within Psest_1519at 1617.996 kb on + strand, within Psest_1519at 1617.996 kb on + strand, within Psest_1519

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Dimethyl Sulfoxide 5 vol%
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1,616,983 + -1.3
1,616,983 + -2.0
1,617,118 + -0.3
1,617,181 - Psest_1518 0.52 -0.5
1,617,196 + Psest_1518 0.69 -1.7
1,617,196 + Psest_1518 0.69 -1.4
1,617,197 - Psest_1518 0.70 -1.4
1,617,225 + -0.1
1,617,225 + -0.0
1,617,225 + -1.0
1,617,225 + -0.3
1,617,225 + -0.1
1,617,225 + +0.3
1,617,225 + +0.7
1,617,225 + +0.7
1,617,225 + -0.2
1,617,226 - -0.1
1,617,226 - -0.4
1,617,226 - -0.1
1,617,226 - +0.7
1,617,226 - +0.3
1,617,226 - -1.1
1,617,226 - +0.1
1,617,226 - -0.1
1,617,231 + -0.0
1,617,232 - +1.1
1,617,232 - +0.0
1,617,232 - +1.4
1,617,427 + -1.8
1,617,428 - +0.0
1,617,449 + -0.3
1,617,652 - Psest_1519 0.11 +0.6
1,617,652 - Psest_1519 0.11 +0.2
1,617,903 + Psest_1519 0.22 +0.4
1,617,903 + Psest_1519 0.22 -0.1
1,617,903 + Psest_1519 0.22 +0.1
1,617,904 - Psest_1519 0.22 -0.3
1,617,942 + Psest_1519 0.23 +0.4
1,617,942 + Psest_1519 0.23 +0.6
1,617,942 + Psest_1519 0.23 -0.4
1,617,942 + Psest_1519 0.23 +0.6
1,617,942 + Psest_1519 0.23 +0.4
1,617,942 + Psest_1519 0.23 -0.8
1,617,942 + Psest_1519 0.23 -0.3
1,617,942 + Psest_1519 0.23 +0.8
1,617,942 + Psest_1519 0.23 +0.9
1,617,942 + Psest_1519 0.23 -0.1
1,617,943 - Psest_1519 0.23 +0.0
1,617,943 - Psest_1519 0.23 -0.3
1,617,943 - Psest_1519 0.23 -0.4
1,617,943 - Psest_1519 0.23 -1.1
1,617,943 - Psest_1519 0.23 +0.2
1,617,943 - Psest_1519 0.23 -0.0
1,617,943 - Psest_1519 0.23 +0.5
1,617,943 - Psest_1519 0.23 -0.4
1,617,943 - Psest_1519 0.23 -0.4
1,617,944 + Psest_1519 0.23 +0.6
1,617,944 + Psest_1519 0.23 -0.0
1,617,944 + Psest_1519 0.23 +1.3
1,617,944 + Psest_1519 0.23 -0.7
1,617,944 + Psest_1519 0.23 +0.2
1,617,944 + Psest_1519 0.23 -0.4
1,617,996 + Psest_1519 0.26 +0.1
1,617,996 + Psest_1519 0.26 -0.4

Or see this region's nucleotide sequence