Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0064 and Psest_0065 are separated by 271 nucleotides Psest_0065 and Psest_0066 are separated by 276 nucleotides Psest_0066 and Psest_0067 are separated by 8 nucleotides
Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053
_0064
Psest_0065: Psest_0065 - Subtilisin-like serine proteases, at 65,325 to 66,716
_0065
Psest_0066: Psest_0066 - Protein of unknown function (DUF3060)., at 66,993 to 67,493
_0066
Psest_0067: Psest_0067 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 67,502 to 68,500
_0067
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 65.099 kb on + strand at 65.099 kb on + strand at 65.179 kb on + strand at 65.246 kb on + strand at 65.246 kb on + strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.683 kb on - strand, within Psest_0065 at 65.724 kb on - strand, within Psest_0065 at 65.769 kb on + strand, within Psest_0065 at 65.994 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.163 kb on - strand, within Psest_0065 at 66.163 kb on - strand, within Psest_0065 at 66.250 kb on - strand, within Psest_0065 at 66.250 kb on - strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.420 kb on + strand, within Psest_0065 at 66.421 kb on - strand, within Psest_0065 at 66.421 kb on - strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.561 kb on + strand, within Psest_0065 at 66.562 kb on - strand, within Psest_0065 at 66.572 kb on - strand, within Psest_0065 at 66.652 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.723 kb on + strand at 66.724 kb on - strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.857 kb on - strand at 66.857 kb on - strand at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand at 67.422 kb on - strand, within Psest_0066 at 67.422 kb on - strand, within Psest_0066 at 67.699 kb on - strand, within Psest_0067
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 64,829 + Psest_0064 0.75 +1.0 64,829 + Psest_0064 0.75 +0.0 64,829 + Psest_0064 0.75 +0.0 64,829 + Psest_0064 0.75 +0.0 64,830 - Psest_0064 0.75 -0.4 64,830 - Psest_0064 0.75 -0.4 64,830 - Psest_0064 0.75 +1.0 64,830 - Psest_0064 0.75 +0.6 64,830 - Psest_0064 0.75 -0.7 65,099 + +0.9 65,099 + -2.0 65,179 + +1.6 65,246 + +0.0 65,246 + -1.0 65,247 - -0.1 65,247 - +1.0 65,247 - -0.1 65,269 + +0.1 65,269 + +0.2 65,269 + -1.5 65,270 - +0.2 65,270 - -0.4 65,270 - +0.0 65,270 - +1.6 65,683 - Psest_0065 0.26 -0.5 65,724 - Psest_0065 0.29 +0.4 65,769 + Psest_0065 0.32 +1.0 65,994 + Psest_0065 0.48 -0.6 66,162 + Psest_0065 0.60 +0.7 66,162 + Psest_0065 0.60 +0.2 66,162 + Psest_0065 0.60 +0.0 66,162 + Psest_0065 0.60 +1.9 66,162 + Psest_0065 0.60 +0.6 66,163 - Psest_0065 0.60 +0.6 66,163 - Psest_0065 0.60 +1.0 66,250 - Psest_0065 0.66 -0.4 66,250 - Psest_0065 0.66 +0.3 66,351 + Psest_0065 0.74 -0.2 66,351 + Psest_0065 0.74 -0.4 66,351 + Psest_0065 0.74 +0.3 66,352 - Psest_0065 0.74 +0.6 66,352 - Psest_0065 0.74 +0.7 66,352 - Psest_0065 0.74 +0.0 66,420 + Psest_0065 0.79 -0.4 66,421 - Psest_0065 0.79 -1.3 66,421 - Psest_0065 0.79 +0.0 66,507 + Psest_0065 0.85 -0.5 66,507 + Psest_0065 0.85 +0.6 66,507 + Psest_0065 0.85 -0.9 66,507 + Psest_0065 0.85 -1.4 66,508 - Psest_0065 0.85 -0.5 66,508 - Psest_0065 0.85 -0.2 66,508 - Psest_0065 0.85 +0.8 66,561 + Psest_0065 0.89 +1.6 66,562 - Psest_0065 0.89 +0.3 66,572 - Psest_0065 0.90 +0.8 66,652 + -0.9 66,719 + +1.4 66,719 + -1.2 66,719 + +1.0 66,719 + +1.6 66,720 - +0.9 66,720 - -0.5 66,723 + -0.2 66,724 - -0.4 66,856 + +0.1 66,856 + -0.9 66,856 + -2.1 66,856 + +0.3 66,856 + -1.1 66,856 + +0.6 66,856 + +1.4 66,857 - +2.2 66,857 - +0.9 67,421 + Psest_0066 0.85 +1.6 67,421 + Psest_0066 0.85 +0.9 67,421 + +0.6 67,422 - Psest_0066 0.86 -0.2 67,422 - Psest_0066 0.86 +1.6 67,699 - Psest_0067 0.20 +1.4
Or see this region's nucleotide sequence