Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0063

Experiment: Def Fusidic 0.064 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0061 and Psest_0062 are separated by 175 nucleotidesPsest_0062 and Psest_0063 overlap by 4 nucleotidesPsest_0063 and Psest_0064 are separated by 171 nucleotides Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786 _0061 Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771 _0062 Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979 _0063 Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053 _0064 Position (kb) 62 63 64Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 61.896 kb on + strandat 61.897 kb on - strandat 61.900 kb on + strandat 61.900 kb on + strandat 61.900 kb on + strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.901 kb on - strandat 61.980 kb on + strandat 61.980 kb on + strandat 61.981 kb on - strandat 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.245 kb on + strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.246 kb on - strand, within Psest_0062at 62.248 kb on + strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.249 kb on - strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.464 kb on + strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.465 kb on - strand, within Psest_0062at 62.684 kb on - strand, within Psest_0062at 62.992 kb on - strand, within Psest_0063at 62.992 kb on - strand, within Psest_0063at 63.405 kb on + strand, within Psest_0063at 63.405 kb on + strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.406 kb on - strand, within Psest_0063at 63.564 kb on + strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.565 kb on - strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.741 kb on + strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.742 kb on - strand, within Psest_0063at 63.787 kb on - strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.810 kb on + strand, within Psest_0063at 63.811 kb on - strand, within Psest_0063at 63.811 kb on - strand, within Psest_0063at 63.826 kb on - strand, within Psest_0063at 63.826 kb on - strand, within Psest_0063at 64.086 kb on + strandat 64.150 kb on + strandat 64.150 kb on + strandat 64.209 kb on + strandat 64.210 kb on - strandat 64.210 kb on - strandat 64.210 kb on - strandat 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml
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61,896 + -0.4
61,897 - -0.9
61,900 + -0.8
61,900 + -1.7
61,900 + +1.6
61,901 - +0.8
61,901 - -0.1
61,901 - -0.1
61,901 - +0.5
61,901 - -1.0
61,980 + +0.4
61,980 + +0.0
61,981 - -0.8
62,245 + Psest_0062 0.35 -2.8
62,245 + Psest_0062 0.35 -4.3
62,245 + Psest_0062 0.35 -2.5
62,245 + Psest_0062 0.35 -0.8
62,246 - Psest_0062 0.35 +0.0
62,246 - Psest_0062 0.35 -2.2
62,246 - Psest_0062 0.35 -0.7
62,246 - Psest_0062 0.35 -0.7
62,246 - Psest_0062 0.35 -2.1
62,248 + Psest_0062 0.35 -2.5
62,249 - Psest_0062 0.35 -2.0
62,249 - Psest_0062 0.35 -1.7
62,249 - Psest_0062 0.35 -2.2
62,464 + Psest_0062 0.62 -4.6
62,464 + Psest_0062 0.62 -4.0
62,464 + Psest_0062 0.62 -3.0
62,465 - Psest_0062 0.62 -2.8
62,465 - Psest_0062 0.62 -1.3
62,465 - Psest_0062 0.62 -4.3
62,465 - Psest_0062 0.62 -3.3
62,465 - Psest_0062 0.62 -1.4
62,465 - Psest_0062 0.62 -1.0
62,465 - Psest_0062 0.62 -1.0
62,465 - Psest_0062 0.62 -2.5
62,465 - Psest_0062 0.62 -2.2
62,465 - Psest_0062 0.62 -0.4
62,465 - Psest_0062 0.62 -2.4
62,465 - Psest_0062 0.62 -0.4
62,684 - Psest_0062 0.89 -3.7
62,992 - Psest_0063 0.18 -2.8
62,992 - Psest_0063 0.18 -3.9
63,405 + Psest_0063 0.53 +0.6
63,405 + Psest_0063 0.53 -1.4
63,406 - Psest_0063 0.53 -1.4
63,406 - Psest_0063 0.53 -1.5
63,406 - Psest_0063 0.53 -4.2
63,406 - Psest_0063 0.53 -2.5
63,406 - Psest_0063 0.53 -0.4
63,406 - Psest_0063 0.53 -0.4
63,406 - Psest_0063 0.53 -1.0
63,406 - Psest_0063 0.53 -2.0
63,564 + Psest_0063 0.66 -2.2
63,565 - Psest_0063 0.66 -2.1
63,565 - Psest_0063 0.66 -3.2
63,565 - Psest_0063 0.66 -1.2
63,565 - Psest_0063 0.66 -2.0
63,565 - Psest_0063 0.66 -2.8
63,741 + Psest_0063 0.80 -2.3
63,741 + Psest_0063 0.80 -0.7
63,741 + Psest_0063 0.80 +0.6
63,741 + Psest_0063 0.80 -1.2
63,741 + Psest_0063 0.80 -2.6
63,742 - Psest_0063 0.80 -4.0
63,742 - Psest_0063 0.80 -1.8
63,742 - Psest_0063 0.80 +1.6
63,742 - Psest_0063 0.80 -2.3
63,787 - Psest_0063 0.84 -1.2
63,810 + Psest_0063 0.86 +0.6
63,810 + Psest_0063 0.86 -0.4
63,810 + Psest_0063 0.86 -3.1
63,810 + Psest_0063 0.86 -3.7
63,810 + Psest_0063 0.86 -0.7
63,810 + Psest_0063 0.86 +0.6
63,810 + Psest_0063 0.86 -1.8
63,810 + Psest_0063 0.86 -2.3
63,811 - Psest_0063 0.86 -0.7
63,811 - Psest_0063 0.86 -1.5
63,826 - Psest_0063 0.87 -4.5
63,826 - Psest_0063 0.87 -3.9
64,086 + -0.2
64,150 + -0.4
64,150 + +0.0
64,209 + -0.7
64,210 - +0.2
64,210 - +0.1
64,210 - +0.0
64,829 + Psest_0064 0.75 +1.0
64,829 + Psest_0064 0.75 +0.0
64,829 + Psest_0064 0.75 +0.0
64,829 + Psest_0064 0.75 +0.0
64,830 - Psest_0064 0.75 -0.4
64,830 - Psest_0064 0.75 -0.4
64,830 - Psest_0064 0.75 +1.0
64,830 - Psest_0064 0.75 +0.6
64,830 - Psest_0064 0.75 -0.7

Or see this region's nucleotide sequence