Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4105 and Psest_4106 are separated by 57 nucleotides Psest_4106 and Psest_4107 are separated by 130 nucleotides Psest_4107 and Psest_4108 overlap by 4 nucleotides
Psest_4105: Psest_4105 - formate dehydrogenase, beta subunit, Fe-S containing, at 4,286,820 to 4,287,758
_4105
Psest_4106: Psest_4106 - formate dehydrogenase, gamma subunit, at 4,287,816 to 4,288,439
_4106
Psest_4107: Psest_4107 - formate dehydrogenase accessory protein FdhE, at 4,288,570 to 4,289,487
_4107
Psest_4108: Psest_4108 - seryl-tRNA(sec) selenium transferase, at 4,289,484 to 4,290,893
_4108
Position (kb)
4288
4289
4290 Strain fitness (log2 ratio)
-1
0
1 at 4287.841 kb on - strand at 4287.841 kb on - strand at 4287.841 kb on - strand at 4287.841 kb on - strand at 4287.841 kb on - strand at 4288.137 kb on + strand, within Psest_4106 at 4288.225 kb on - strand, within Psest_4106 at 4288.323 kb on + strand, within Psest_4106 at 4288.323 kb on + strand, within Psest_4106 at 4288.323 kb on + strand, within Psest_4106 at 4288.324 kb on - strand, within Psest_4106 at 4288.437 kb on + strand at 4288.437 kb on + strand at 4288.437 kb on + strand at 4288.438 kb on - strand at 4288.532 kb on + strand at 4288.532 kb on + strand at 4288.532 kb on + strand at 4288.533 kb on - strand at 4288.533 kb on - strand at 4288.586 kb on + strand at 4288.586 kb on + strand at 4288.586 kb on + strand at 4288.586 kb on + strand at 4288.586 kb on + strand at 4288.923 kb on - strand, within Psest_4107 at 4289.227 kb on + strand, within Psest_4107 at 4289.227 kb on + strand, within Psest_4107 at 4289.228 kb on - strand, within Psest_4107 at 4289.228 kb on - strand, within Psest_4107 at 4289.228 kb on - strand, within Psest_4107 at 4289.228 kb on - strand, within Psest_4107 at 4289.275 kb on + strand, within Psest_4107 at 4289.338 kb on + strand, within Psest_4107 at 4289.338 kb on + strand, within Psest_4107 at 4289.338 kb on + strand, within Psest_4107 at 4289.353 kb on + strand, within Psest_4107 at 4289.353 kb on + strand, within Psest_4107 at 4289.353 kb on + strand, within Psest_4107 at 4289.354 kb on - strand, within Psest_4107 at 4289.354 kb on - strand, within Psest_4107 at 4290.102 kb on + strand, within Psest_4108 at 4290.103 kb on - strand, within Psest_4108 at 4290.159 kb on + strand, within Psest_4108 at 4290.159 kb on + strand, within Psest_4108 at 4290.160 kb on - strand, within Psest_4108 at 4290.160 kb on - strand, within Psest_4108 at 4290.160 kb on - strand, within Psest_4108 at 4290.160 kb on - strand, within Psest_4108 at 4290.160 kb on - strand, within Psest_4108
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 4,287,841 - +0.3 4,287,841 - +0.2 4,287,841 - -0.6 4,287,841 - +0.2 4,287,841 - -0.3 4,288,137 + Psest_4106 0.51 -1.7 4,288,225 - Psest_4106 0.66 -0.4 4,288,323 + Psest_4106 0.81 -0.6 4,288,323 + Psest_4106 0.81 -1.4 4,288,323 + Psest_4106 0.81 +0.0 4,288,324 - Psest_4106 0.81 -0.0 4,288,437 + -0.6 4,288,437 + +1.2 4,288,437 + +0.9 4,288,438 - +1.6 4,288,532 + -0.9 4,288,532 + +0.4 4,288,532 + -0.2 4,288,533 - +0.5 4,288,533 - -0.2 4,288,586 + +0.4 4,288,586 + +1.2 4,288,586 + -0.8 4,288,586 + -1.2 4,288,586 + -1.4 4,288,923 - Psest_4107 0.38 -1.6 4,289,227 + Psest_4107 0.72 +0.0 4,289,227 + Psest_4107 0.72 +0.6 4,289,228 - Psest_4107 0.72 -1.4 4,289,228 - Psest_4107 0.72 -1.3 4,289,228 - Psest_4107 0.72 -1.0 4,289,228 - Psest_4107 0.72 +1.0 4,289,275 + Psest_4107 0.77 -1.4 4,289,338 + Psest_4107 0.84 -0.4 4,289,338 + Psest_4107 0.84 +0.9 4,289,338 + Psest_4107 0.84 -1.2 4,289,353 + Psest_4107 0.85 +1.2 4,289,353 + Psest_4107 0.85 -0.4 4,289,353 + Psest_4107 0.85 -0.1 4,289,354 - Psest_4107 0.85 +0.6 4,289,354 - Psest_4107 0.85 -1.3 4,290,102 + Psest_4108 0.44 -0.4 4,290,103 - Psest_4108 0.44 -1.2 4,290,159 + Psest_4108 0.48 +0.6 4,290,159 + Psest_4108 0.48 -1.4 4,290,160 - Psest_4108 0.48 -0.8 4,290,160 - Psest_4108 0.48 +1.6 4,290,160 - Psest_4108 0.48 -0.1 4,290,160 - Psest_4108 0.48 +1.6 4,290,160 - Psest_4108 0.48 -0.1
Or see this region's nucleotide sequence