Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0264 and Psest_0265 are separated by 68 nucleotides Psest_0265 and Psest_0266 are separated by 88 nucleotides Psest_0266 and Psest_0267 are separated by 74 nucleotides Psest_0267 and Psest_0268 are separated by 65 nucleotides
Psest_0264: Psest_0264 - monofunctional biosynthetic peptidoglycan transglycosylase, at 281,399 to 282,127
_0264
Psest_0265: Psest_0265 - Uncharacterized small membrane protein, at 282,196 to 282,570
_0265
Psest_0266: Psest_0266 - thiamine biosynthesis protein ThiS, at 282,659 to 282,859
_0266
Psest_0267: Psest_0267 - Uncharacterized enzyme of thiazole biosynthesis, at 282,934 to 283,728
_0267
Psest_0268: Psest_0268 - tRNA (guanine-N(7)-)-methyltransferase, at 283,794 to 284,510
_0268
Position (kb) 282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.974 kb on + strand, within Psest_0264 at 282.056 kb on + strand at 282.056 kb on + strand at 282.057 kb on - strand at 282.057 kb on - strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.162 kb on + strand at 282.163 kb on - strand at 282.208 kb on + strand at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.329 kb on - strand, within Psest_0265 at 282.454 kb on + strand, within Psest_0265 at 282.454 kb on + strand, within Psest_0265 at 282.455 kb on - strand, within Psest_0265 at 282.622 kb on - strand at 282.686 kb on + strand, within Psest_0266 at 282.686 kb on + strand, within Psest_0266 at 282.687 kb on - strand, within Psest_0266 at 282.726 kb on + strand, within Psest_0266 at 282.727 kb on - strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.889 kb on + strand at 282.890 kb on - strand at 282.892 kb on - strand at 282.909 kb on + strand at 282.909 kb on + strand at 282.910 kb on - strand at 282.982 kb on + strand at 282.982 kb on + strand at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.150 kb on + strand, within Psest_0267 at 283.151 kb on - strand, within Psest_0267 at 283.280 kb on + strand, within Psest_0267 at 283.280 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.459 kb on + strand, within Psest_0267 at 283.459 kb on + strand, within Psest_0267 at 283.460 kb on - strand, within Psest_0267 at 283.460 kb on - strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.658 kb on - strand at 283.687 kb on + strand at 283.788 kb on + strand at 283.788 kb on + strand at 283.789 kb on - strand at 283.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 281,673 + Psest_0264 0.38 -1.7 281,673 + Psest_0264 0.38 -0.4 281,673 + Psest_0264 0.38 +0.9 281,673 + Psest_0264 0.38 +1.1 281,674 - Psest_0264 0.38 +0.1 281,674 - Psest_0264 0.38 +1.0 281,674 - Psest_0264 0.38 +0.3 281,674 - Psest_0264 0.38 -0.9 281,674 - Psest_0264 0.38 +0.6 281,674 - Psest_0264 0.38 -1.1 281,674 - Psest_0264 0.38 -0.7 281,674 - Psest_0264 0.38 -0.9 281,974 + Psest_0264 0.79 -0.2 282,056 + -1.6 282,056 + +0.6 282,057 - -0.7 282,057 - -0.6 282,160 + +0.8 282,160 + -0.8 282,160 + +0.1 282,161 - -0.1 282,161 - +0.2 282,161 - -0.2 282,161 - -0.1 282,161 - -0.6 282,162 + -0.6 282,163 - +0.1 282,208 + +0.4 282,299 - Psest_0265 0.27 +0.5 282,299 - Psest_0265 0.27 +1.5 282,299 - Psest_0265 0.27 +0.3 282,299 - Psest_0265 0.27 +0.0 282,299 - Psest_0265 0.27 +0.4 282,299 - Psest_0265 0.27 -1.1 282,329 - Psest_0265 0.35 -1.1 282,454 + Psest_0265 0.69 -1.8 282,454 + Psest_0265 0.69 -0.4 282,455 - Psest_0265 0.69 -0.8 282,622 - +0.6 282,686 + Psest_0266 0.13 -0.5 282,686 + Psest_0266 0.13 -0.9 282,687 - Psest_0266 0.14 -0.2 282,726 + Psest_0266 0.33 -0.9 282,727 - Psest_0266 0.34 -0.7 282,837 + Psest_0266 0.89 -0.1 282,837 + Psest_0266 0.89 -1.2 282,837 + Psest_0266 0.89 -1.4 282,837 + Psest_0266 0.89 -1.4 282,838 - Psest_0266 0.89 -1.5 282,838 - Psest_0266 0.89 -0.8 282,838 - Psest_0266 0.89 -1.3 282,838 - Psest_0266 0.89 +1.1 282,838 - Psest_0266 0.89 +0.5 282,838 - Psest_0266 0.89 -1.9 282,838 - Psest_0266 0.89 -1.6 282,889 + +0.2 282,890 - +0.3 282,892 - +0.0 282,909 + -0.2 282,909 + -0.9 282,910 - +0.3 282,982 + -0.9 282,982 + +0.2 283,015 + Psest_0267 0.10 -1.4 283,015 + Psest_0267 0.10 -0.5 283,015 + Psest_0267 0.10 -0.9 283,015 + Psest_0267 0.10 -0.8 283,150 + Psest_0267 0.27 -1.3 283,151 - Psest_0267 0.27 -0.5 283,280 + Psest_0267 0.44 -1.9 283,280 + Psest_0267 0.44 -0.5 283,351 + Psest_0267 0.52 -0.4 283,351 + Psest_0267 0.52 -1.5 283,351 + Psest_0267 0.52 -0.3 283,352 - Psest_0267 0.53 -0.9 283,352 - Psest_0267 0.53 -0.8 283,352 - Psest_0267 0.53 -2.8 283,352 - Psest_0267 0.53 -2.5 283,352 - Psest_0267 0.53 -0.2 283,352 - Psest_0267 0.53 -1.0 283,352 - Psest_0267 0.53 -1.6 283,352 - Psest_0267 0.53 +0.7 283,352 - Psest_0267 0.53 -0.8 283,352 - Psest_0267 0.53 -1.0 283,459 + Psest_0267 0.66 -1.0 283,459 + Psest_0267 0.66 -1.9 283,460 - Psest_0267 0.66 -0.5 283,460 - Psest_0267 0.66 -0.1 283,630 + Psest_0267 0.88 -0.4 283,630 + Psest_0267 0.88 -1.3 283,630 + Psest_0267 0.88 -0.8 283,630 + Psest_0267 0.88 -2.9 283,630 + Psest_0267 0.88 -2.0 283,630 + Psest_0267 0.88 -0.5 283,631 - Psest_0267 0.88 -0.4 283,631 - Psest_0267 0.88 -0.9 283,631 - Psest_0267 0.88 +1.1 283,658 - -0.4 283,687 + -1.0 283,788 + +0.9 283,788 + -0.1 283,789 - +0.9 283,789 - +0.2
Or see this region's nucleotide sequence