Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2446 and Psest_2447 are separated by 11 nucleotides Psest_2447 and Psest_2448 are separated by 186 nucleotides
Psest_2446: Psest_2446 - acetyl-CoA acetyltransferases, at 2,615,025 to 2,616,209
_2446
Psest_2447: Psest_2447 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,616,221 to 2,616,988
_2447
Psest_2448: Psest_2448 - AraC-type DNA-binding domain-containing proteins, at 2,617,175 to 2,618,242
_2448
Position (kb)
2616
2617 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2615.749 kb on + strand, within Psest_2446 at 2615.836 kb on + strand, within Psest_2446 at 2615.837 kb on - strand, within Psest_2446 at 2615.837 kb on - strand, within Psest_2446 at 2616.171 kb on - strand at 2616.190 kb on - strand at 2616.222 kb on + strand at 2616.223 kb on - strand at 2616.313 kb on - strand, within Psest_2447 at 2616.410 kb on + strand, within Psest_2447 at 2616.508 kb on - strand, within Psest_2447 at 2616.508 kb on - strand, within Psest_2447 at 2616.968 kb on + strand at 2616.968 kb on + strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2617.033 kb on - strand at 2617.225 kb on + strand at 2617.251 kb on + strand at 2617.252 kb on - strand at 2617.526 kb on + strand, within Psest_2448 at 2617.526 kb on + strand, within Psest_2448 at 2617.527 kb on - strand, within Psest_2448 at 2617.680 kb on + strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.780 kb on - strand, within Psest_2448
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 2,615,749 + Psest_2446 0.61 +0.4 2,615,836 + Psest_2446 0.68 +1.4 2,615,837 - Psest_2446 0.69 +0.4 2,615,837 - Psest_2446 0.69 +0.4 2,616,171 - -0.2 2,616,190 - +2.6 2,616,222 + +0.4 2,616,223 - -0.4 2,616,313 - Psest_2447 0.12 -0.6 2,616,410 + Psest_2447 0.25 -0.2 2,616,508 - Psest_2447 0.37 -0.6 2,616,508 - Psest_2447 0.37 -1.4 2,616,968 + +0.3 2,616,968 + +0.7 2,616,969 - +0.4 2,616,969 - +0.4 2,616,969 - +0.2 2,616,969 - +1.0 2,617,033 - +1.4 2,617,225 + -0.5 2,617,251 + -0.1 2,617,252 - +3.0 2,617,526 + Psest_2448 0.33 -0.1 2,617,526 + Psest_2448 0.33 +0.1 2,617,527 - Psest_2448 0.33 +1.4 2,617,680 + Psest_2448 0.47 +0.9 2,617,681 - Psest_2448 0.47 +0.4 2,617,681 - Psest_2448 0.47 +0.4 2,617,779 + Psest_2448 0.57 -1.3 2,617,779 + Psest_2448 0.57 +1.0 2,617,779 + Psest_2448 0.57 -0.7 2,617,780 - Psest_2448 0.57 +0.7
Or see this region's nucleotide sequence