Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2223 and Psest_2225 are separated by 532 nucleotides Psest_2225 and Psest_2226 are separated by 54 nucleotides
Psest_2223: Psest_2223 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 2,373,246 to 2,373,995
_2223
Psest_2225: Psest_2225 - Diacylglycerol kinase, at 2,374,528 to 2,374,914
_2225
Psest_2226: Psest_2226 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 2,374,969 to 2,377,005
_2226
Position (kb)
2374
2375 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2373.669 kb on - strand, within Psest_2223 at 2373.834 kb on + strand, within Psest_2223 at 2373.834 kb on + strand, within Psest_2223 at 2373.834 kb on + strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2373.835 kb on - strand, within Psest_2223 at 2374.013 kb on + strand at 2374.014 kb on - strand at 2374.051 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.103 kb on + strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.104 kb on - strand at 2374.106 kb on + strand at 2374.106 kb on + strand at 2374.143 kb on + strand at 2374.143 kb on + strand at 2374.161 kb on - strand at 2374.161 kb on - strand at 2374.291 kb on - strand at 2374.299 kb on + strand at 2374.299 kb on + strand at 2374.300 kb on - strand at 2374.300 kb on - strand at 2374.300 kb on - strand at 2374.305 kb on - strand at 2374.496 kb on + strand at 2374.497 kb on - strand at 2374.497 kb on - strand at 2374.497 kb on - strand at 2374.498 kb on + strand at 2374.498 kb on + strand at 2374.499 kb on - strand at 2374.499 kb on - strand at 2374.527 kb on + strand at 2374.528 kb on - strand at 2374.620 kb on + strand, within Psest_2225 at 2374.661 kb on + strand, within Psest_2225 at 2374.662 kb on - strand, within Psest_2225 at 2374.662 kb on - strand, within Psest_2225 at 2374.709 kb on + strand, within Psest_2225 at 2374.910 kb on - strand at 2374.991 kb on + strand at 2374.991 kb on + strand at 2375.194 kb on + strand, within Psest_2226 at 2375.194 kb on + strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.195 kb on - strand, within Psest_2226 at 2375.546 kb on + strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.547 kb on - strand, within Psest_2226 at 2375.666 kb on + strand, within Psest_2226 at 2375.682 kb on + strand, within Psest_2226 at 2375.705 kb on - strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.786 kb on + strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.787 kb on - strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.861 kb on + strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.862 kb on - strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.864 kb on + strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226 at 2375.865 kb on - strand, within Psest_2226
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 2,373,669 - Psest_2223 0.56 +0.6 2,373,834 + Psest_2223 0.78 +0.1 2,373,834 + Psest_2223 0.78 -1.4 2,373,834 + Psest_2223 0.78 +0.6 2,373,835 - Psest_2223 0.79 -0.9 2,373,835 - Psest_2223 0.79 -0.2 2,373,835 - Psest_2223 0.79 +0.0 2,373,835 - Psest_2223 0.79 -1.0 2,373,835 - Psest_2223 0.79 -1.7 2,373,835 - Psest_2223 0.79 +0.6 2,374,013 + -0.1 2,374,014 - -0.7 2,374,051 + +0.1 2,374,103 + -0.5 2,374,103 + +0.8 2,374,103 + -0.0 2,374,103 + -1.1 2,374,103 + -0.8 2,374,103 + +0.2 2,374,104 - +0.4 2,374,104 - -0.7 2,374,104 - +0.4 2,374,104 - +0.8 2,374,104 - +0.2 2,374,104 - +0.2 2,374,104 - +0.4 2,374,106 + +0.7 2,374,106 + +0.0 2,374,143 + -0.2 2,374,143 + +0.8 2,374,161 - +0.5 2,374,161 - +1.4 2,374,291 - +0.5 2,374,299 + -0.2 2,374,299 + +0.2 2,374,300 - -1.6 2,374,300 - +0.8 2,374,300 - -0.0 2,374,305 - +2.0 2,374,496 + +0.3 2,374,497 - +0.0 2,374,497 - -0.5 2,374,497 - -1.0 2,374,498 + +0.3 2,374,498 + +0.5 2,374,499 - -0.2 2,374,499 - +0.9 2,374,527 + +0.2 2,374,528 - +0.4 2,374,620 + Psest_2225 0.24 -2.3 2,374,661 + Psest_2225 0.34 -1.4 2,374,662 - Psest_2225 0.35 -0.8 2,374,662 - Psest_2225 0.35 -0.6 2,374,709 + Psest_2225 0.47 -2.1 2,374,910 - -0.6 2,374,991 + -0.2 2,374,991 + +0.2 2,375,194 + Psest_2226 0.11 -0.6 2,375,194 + Psest_2226 0.11 -0.0 2,375,195 - Psest_2226 0.11 -0.6 2,375,195 - Psest_2226 0.11 -0.4 2,375,195 - Psest_2226 0.11 +1.2 2,375,546 + Psest_2226 0.28 +0.0 2,375,547 - Psest_2226 0.28 -1.1 2,375,547 - Psest_2226 0.28 -0.5 2,375,547 - Psest_2226 0.28 +0.5 2,375,666 + Psest_2226 0.34 -1.2 2,375,682 + Psest_2226 0.35 +0.5 2,375,705 - Psest_2226 0.36 -1.1 2,375,786 + Psest_2226 0.40 +0.6 2,375,786 + Psest_2226 0.40 +0.2 2,375,786 + Psest_2226 0.40 -1.4 2,375,786 + Psest_2226 0.40 +0.2 2,375,786 + Psest_2226 0.40 +0.3 2,375,787 - Psest_2226 0.40 -0.6 2,375,787 - Psest_2226 0.40 -0.2 2,375,787 - Psest_2226 0.40 -1.0 2,375,861 + Psest_2226 0.44 +0.4 2,375,861 + Psest_2226 0.44 -0.4 2,375,861 + Psest_2226 0.44 +0.2 2,375,861 + Psest_2226 0.44 +0.6 2,375,861 + Psest_2226 0.44 +0.0 2,375,861 + Psest_2226 0.44 -1.7 2,375,862 - Psest_2226 0.44 -0.2 2,375,862 - Psest_2226 0.44 -0.8 2,375,862 - Psest_2226 0.44 +0.2 2,375,862 - Psest_2226 0.44 -1.8 2,375,862 - Psest_2226 0.44 -1.0 2,375,862 - Psest_2226 0.44 +0.1 2,375,864 + Psest_2226 0.44 -0.2 2,375,864 + Psest_2226 0.44 -0.6 2,375,864 + Psest_2226 0.44 -0.6 2,375,865 - Psest_2226 0.44 -0.2 2,375,865 - Psest_2226 0.44 +0.2 2,375,865 - Psest_2226 0.44 +0.1 2,375,865 - Psest_2226 0.44 +0.4
Or see this region's nucleotide sequence