Experiment: Def Fusidic 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1773 and Psest_1774 are separated by 28 nucleotides Psest_1774 and Psest_1775 are separated by 85 nucleotides Psest_1775 and Psest_1776 are separated by 52 nucleotides Psest_1776 and Psest_1777 are separated by 57 nucleotides
Psest_1773: Psest_1773 - methionine-R-sulfoxide reductase, at 1,874,342 to 1,874,740
_1773
Psest_1774: Psest_1774 - Glutathione peroxidase, at 1,874,769 to 1,875,251
_1774
Psest_1775: Psest_1775 - Predicted ATPase (AAA+ superfamily), at 1,875,337 to 1,876,227
_1775
Psest_1776: Psest_1776 - Glutathione S-transferase, at 1,876,280 to 1,876,957
_1776
Psest_1777: Psest_1777 - hypothetical protein, at 1,877,015 to 1,877,782
_1777
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1874.337 kb on + strand at 1874.338 kb on - strand at 1874.338 kb on - strand at 1874.338 kb on - strand at 1874.447 kb on + strand, within Psest_1773 at 1874.447 kb on + strand, within Psest_1773 at 1874.447 kb on + strand, within Psest_1773 at 1874.448 kb on - strand, within Psest_1773 at 1874.448 kb on - strand, within Psest_1773 at 1874.448 kb on - strand, within Psest_1773 at 1874.549 kb on + strand, within Psest_1773 at 1874.550 kb on - strand, within Psest_1773 at 1874.550 kb on - strand, within Psest_1773 at 1874.895 kb on + strand, within Psest_1774 at 1874.896 kb on - strand, within Psest_1774 at 1875.195 kb on + strand, within Psest_1774 at 1875.289 kb on + strand at 1875.331 kb on + strand at 1875.664 kb on + strand, within Psest_1775 at 1875.665 kb on - strand, within Psest_1775 at 1875.665 kb on - strand, within Psest_1775 at 1875.740 kb on + strand, within Psest_1775 at 1875.740 kb on + strand, within Psest_1775 at 1875.740 kb on + strand, within Psest_1775 at 1875.741 kb on - strand, within Psest_1775 at 1875.741 kb on - strand, within Psest_1775 at 1875.872 kb on - strand, within Psest_1775 at 1876.039 kb on + strand, within Psest_1775 at 1876.361 kb on + strand, within Psest_1776 at 1876.361 kb on + strand, within Psest_1776 at 1876.362 kb on - strand, within Psest_1776 at 1876.362 kb on - strand, within Psest_1776 at 1876.527 kb on + strand, within Psest_1776 at 1876.528 kb on - strand, within Psest_1776 at 1876.630 kb on + strand, within Psest_1776 at 1876.656 kb on + strand, within Psest_1776 at 1876.656 kb on + strand, within Psest_1776 at 1876.656 kb on + strand, within Psest_1776 at 1876.657 kb on - strand, within Psest_1776 at 1876.657 kb on - strand, within Psest_1776 at 1876.666 kb on - strand, within Psest_1776 at 1876.683 kb on - strand, within Psest_1776 at 1876.749 kb on + strand, within Psest_1776 at 1876.833 kb on + strand, within Psest_1776 at 1876.833 kb on + strand, within Psest_1776 at 1876.912 kb on + strand at 1876.913 kb on - strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.957 kb on + strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1876.958 kb on - strand at 1877.019 kb on + strand at 1877.172 kb on + strand, within Psest_1777 at 1877.172 kb on + strand, within Psest_1777 at 1877.173 kb on - strand, within Psest_1777
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.064 mg/ml remove 1,874,337 + +0.1 1,874,338 - +0.2 1,874,338 - -0.1 1,874,338 - -0.4 1,874,447 + Psest_1773 0.26 -0.1 1,874,447 + Psest_1773 0.26 -0.3 1,874,447 + Psest_1773 0.26 -0.2 1,874,448 - Psest_1773 0.27 -0.6 1,874,448 - Psest_1773 0.27 +0.1 1,874,448 - Psest_1773 0.27 -0.2 1,874,549 + Psest_1773 0.52 -2.2 1,874,550 - Psest_1773 0.52 -0.0 1,874,550 - Psest_1773 0.52 +0.3 1,874,895 + Psest_1774 0.26 +0.7 1,874,896 - Psest_1774 0.26 +0.3 1,875,195 + Psest_1774 0.88 +0.8 1,875,289 + -0.1 1,875,331 + +0.1 1,875,664 + Psest_1775 0.37 -0.0 1,875,665 - Psest_1775 0.37 -0.8 1,875,665 - Psest_1775 0.37 -0.8 1,875,740 + Psest_1775 0.45 -0.5 1,875,740 + Psest_1775 0.45 -0.8 1,875,740 + Psest_1775 0.45 -0.8 1,875,741 - Psest_1775 0.45 -1.3 1,875,741 - Psest_1775 0.45 +0.7 1,875,872 - Psest_1775 0.60 -1.3 1,876,039 + Psest_1775 0.79 -1.9 1,876,361 + Psest_1776 0.12 -0.3 1,876,361 + Psest_1776 0.12 +0.8 1,876,362 - Psest_1776 0.12 +0.4 1,876,362 - Psest_1776 0.12 -1.6 1,876,527 + Psest_1776 0.36 -0.6 1,876,528 - Psest_1776 0.37 -1.0 1,876,630 + Psest_1776 0.52 +0.1 1,876,656 + Psest_1776 0.55 -0.1 1,876,656 + Psest_1776 0.55 +0.7 1,876,656 + Psest_1776 0.55 -0.1 1,876,657 - Psest_1776 0.56 +2.0 1,876,657 - Psest_1776 0.56 +0.3 1,876,666 - Psest_1776 0.57 -0.1 1,876,683 - Psest_1776 0.59 -0.7 1,876,749 + Psest_1776 0.69 +0.3 1,876,833 + Psest_1776 0.82 +0.7 1,876,833 + Psest_1776 0.82 +0.0 1,876,912 + -1.2 1,876,913 - +0.2 1,876,957 + -1.2 1,876,957 + -0.8 1,876,957 + +0.4 1,876,957 + +0.9 1,876,957 + -0.3 1,876,957 + +0.7 1,876,957 + -1.8 1,876,958 - -0.3 1,876,958 - -0.3 1,876,958 - -0.2 1,876,958 - -0.2 1,876,958 - +0.8 1,876,958 - -0.1 1,876,958 - +0.1 1,876,958 - +0.5 1,876,958 - +1.1 1,876,958 - -0.1 1,876,958 - -0.7 1,877,019 + +0.3 1,877,172 + Psest_1777 0.20 -0.5 1,877,172 + Psest_1777 0.20 -1.2 1,877,173 - Psest_1777 0.21 +0.1
Or see this region's nucleotide sequence