Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3273 and Psest_3274 overlap by 4 nucleotides Psest_3274 and Psest_3275 are separated by 48 nucleotides Psest_3275 and Psest_3276 are separated by 14 nucleotides Psest_3276 and Psest_3277 are separated by 88 nucleotides
Psest_3273: Psest_3273 - Phosphoheptose isomerase, at 3,442,523 to 3,443,116
_3273
Psest_3274: Psest_3274 - Predicted periplasmic or secreted lipoprotein, at 3,443,113 to 3,443,688
_3274
Psest_3275: Psest_3275 - Stringent starvation protein B, at 3,443,737 to 3,444,141
_3275
Psest_3276: Psest_3276 - Glutathione S-transferase, at 3,444,156 to 3,444,773
_3276
Psest_3277: Psest_3277 - Cytochrome c1, at 3,444,862 to 3,445,641
_3277
Position (kb)
3443
3444
3445 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3443.739 kb on - strand at 3443.739 kb on - strand at 3443.739 kb on - strand at 3443.853 kb on + strand, within Psest_3275 at 3443.876 kb on + strand, within Psest_3275 at 3443.876 kb on + strand, within Psest_3275 at 3443.876 kb on + strand, within Psest_3275 at 3443.876 kb on + strand, within Psest_3275 at 3443.877 kb on - strand, within Psest_3275 at 3443.877 kb on - strand, within Psest_3275 at 3443.946 kb on + strand, within Psest_3275 at 3443.946 kb on + strand, within Psest_3275 at 3443.947 kb on - strand, within Psest_3275 at 3443.947 kb on - strand, within Psest_3275 at 3443.949 kb on + strand, within Psest_3275 at 3443.950 kb on - strand, within Psest_3275 at 3444.005 kb on - strand, within Psest_3275 at 3444.005 kb on - strand, within Psest_3275 at 3444.005 kb on - strand, within Psest_3275 at 3444.005 kb on - strand, within Psest_3275 at 3444.122 kb on + strand at 3444.768 kb on - strand at 3444.774 kb on - strand at 3444.793 kb on + strand at 3444.839 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 3,443,739 - -0.8 3,443,739 - -0.0 3,443,739 - +0.2 3,443,853 + Psest_3275 0.29 -1.4 3,443,876 + Psest_3275 0.34 -0.8 3,443,876 + Psest_3275 0.34 -0.4 3,443,876 + Psest_3275 0.34 -1.4 3,443,876 + Psest_3275 0.34 -2.2 3,443,877 - Psest_3275 0.35 +2.2 3,443,877 - Psest_3275 0.35 -0.8 3,443,946 + Psest_3275 0.52 -0.8 3,443,946 + Psest_3275 0.52 -0.8 3,443,947 - Psest_3275 0.52 -1.4 3,443,947 - Psest_3275 0.52 +0.2 3,443,949 + Psest_3275 0.52 +0.0 3,443,950 - Psest_3275 0.53 +0.2 3,444,005 - Psest_3275 0.66 +0.9 3,444,005 - Psest_3275 0.66 -0.8 3,444,005 - Psest_3275 0.66 -0.8 3,444,005 - Psest_3275 0.66 -1.2 3,444,122 + +0.6 3,444,768 - +1.2 3,444,774 - -0.1 3,444,793 + -0.6 3,444,839 + -1.9
Or see this region's nucleotide sequence