Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0950 and Psest_0951 are separated by 181 nucleotides Psest_0951 and Psest_0952 overlap by 4 nucleotides Psest_0952 and Psest_0953 overlap by 4 nucleotides
Psest_0950: Psest_0950 - hypothetical protein, at 1,030,070 to 1,030,375
_0950
Psest_0951: Psest_0951 - FAD/FMN-containing dehydrogenases, at 1,030,557 to 1,033,409
_0951
Psest_0952: Psest_0952 - Uncharacterized conserved protein, at 1,033,406 to 1,034,077
_0952
Psest_0953: Psest_0953 - iron-sulfur cluster-binding protein, at 1,034,074 to 1,035,534
_0953
Position (kb)
1030
1031
1032
1033
1034 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1029.613 kb on - strand at 1029.849 kb on - strand at 1029.864 kb on + strand at 1030.001 kb on + strand at 1030.215 kb on + strand, within Psest_0950 at 1030.215 kb on + strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.216 kb on - strand, within Psest_0950 at 1030.558 kb on + strand at 1030.580 kb on + strand at 1030.606 kb on + strand at 1030.607 kb on - strand at 1030.689 kb on + strand at 1030.707 kb on - strand at 1030.925 kb on - strand, within Psest_0951 at 1030.951 kb on + strand, within Psest_0951 at 1031.017 kb on + strand, within Psest_0951 at 1031.017 kb on + strand, within Psest_0951 at 1031.018 kb on - strand, within Psest_0951 at 1031.099 kb on - strand, within Psest_0951 at 1031.620 kb on + strand, within Psest_0951 at 1031.627 kb on - strand, within Psest_0951 at 1032.277 kb on + strand, within Psest_0951 at 1032.277 kb on + strand, within Psest_0951 at 1032.277 kb on + strand, within Psest_0951 at 1032.277 kb on + strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.278 kb on - strand, within Psest_0951 at 1032.604 kb on + strand, within Psest_0951 at 1032.604 kb on + strand, within Psest_0951 at 1032.604 kb on + strand, within Psest_0951 at 1032.604 kb on + strand, within Psest_0951 at 1032.604 kb on + strand, within Psest_0951 at 1032.730 kb on + strand, within Psest_0951 at 1032.730 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.907 kb on + strand, within Psest_0951 at 1032.908 kb on - strand, within Psest_0951 at 1032.908 kb on - strand, within Psest_0951 at 1032.908 kb on - strand, within Psest_0951 at 1032.908 kb on - strand, within Psest_0951 at 1033.212 kb on + strand at 1033.273 kb on + strand at 1033.459 kb on - strand at 1033.459 kb on - strand at 1033.560 kb on + strand, within Psest_0952 at 1033.560 kb on + strand, within Psest_0952 at 1033.972 kb on - strand, within Psest_0952 at 1033.972 kb on - strand, within Psest_0952 at 1033.972 kb on - strand, within Psest_0952 at 1033.972 kb on - strand, within Psest_0952 at 1033.998 kb on - strand, within Psest_0952 at 1034.018 kb on - strand at 1034.220 kb on - strand, within Psest_0953 at 1034.283 kb on - strand, within Psest_0953
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 1,029,613 - -0.2 1,029,849 - -1.0 1,029,864 + -2.4 1,030,001 + -1.9 1,030,215 + Psest_0950 0.47 -1.5 1,030,215 + Psest_0950 0.47 -0.7 1,030,216 - Psest_0950 0.48 +0.2 1,030,216 - Psest_0950 0.48 +0.3 1,030,216 - Psest_0950 0.48 -0.9 1,030,216 - Psest_0950 0.48 +1.8 1,030,216 - Psest_0950 0.48 +0.9 1,030,216 - Psest_0950 0.48 -0.3 1,030,216 - Psest_0950 0.48 -0.0 1,030,558 + -0.1 1,030,580 + +0.6 1,030,606 + -1.4 1,030,607 - -0.8 1,030,689 + -0.9 1,030,707 - -2.2 1,030,925 - Psest_0951 0.13 -2.5 1,030,951 + Psest_0951 0.14 -0.8 1,031,017 + Psest_0951 0.16 -1.3 1,031,017 + Psest_0951 0.16 -1.3 1,031,018 - Psest_0951 0.16 -2.5 1,031,099 - Psest_0951 0.19 +0.0 1,031,620 + Psest_0951 0.37 -1.0 1,031,627 - Psest_0951 0.38 -1.2 1,032,277 + Psest_0951 0.60 +0.2 1,032,277 + Psest_0951 0.60 -0.5 1,032,277 + Psest_0951 0.60 -1.0 1,032,277 + Psest_0951 0.60 +0.3 1,032,278 - Psest_0951 0.60 -1.6 1,032,278 - Psest_0951 0.60 -1.0 1,032,278 - Psest_0951 0.60 -1.0 1,032,278 - Psest_0951 0.60 +0.2 1,032,278 - Psest_0951 0.60 -0.8 1,032,278 - Psest_0951 0.60 -3.1 1,032,278 - Psest_0951 0.60 -0.5 1,032,278 - Psest_0951 0.60 -1.3 1,032,604 + Psest_0951 0.72 -1.8 1,032,604 + Psest_0951 0.72 +0.7 1,032,604 + Psest_0951 0.72 -1.3 1,032,604 + Psest_0951 0.72 -3.9 1,032,604 + Psest_0951 0.72 -2.2 1,032,730 + Psest_0951 0.76 -0.5 1,032,730 + Psest_0951 0.76 -1.7 1,032,907 + Psest_0951 0.82 +0.0 1,032,907 + Psest_0951 0.82 -0.3 1,032,907 + Psest_0951 0.82 -1.4 1,032,907 + Psest_0951 0.82 -1.5 1,032,907 + Psest_0951 0.82 -1.1 1,032,907 + Psest_0951 0.82 -0.5 1,032,907 + Psest_0951 0.82 -0.0 1,032,907 + Psest_0951 0.82 -1.6 1,032,908 - Psest_0951 0.82 -0.8 1,032,908 - Psest_0951 0.82 -1.5 1,032,908 - Psest_0951 0.82 -0.9 1,032,908 - Psest_0951 0.82 -1.4 1,033,212 + -0.5 1,033,273 + -2.5 1,033,459 - -4.9 1,033,459 - -4.8 1,033,560 + Psest_0952 0.23 -1.0 1,033,560 + Psest_0952 0.23 -2.6 1,033,972 - Psest_0952 0.84 -3.0 1,033,972 - Psest_0952 0.84 -3.7 1,033,972 - Psest_0952 0.84 -2.4 1,033,972 - Psest_0952 0.84 -2.5 1,033,998 - Psest_0952 0.88 -2.7 1,034,018 - -1.0 1,034,220 - Psest_0953 0.10 -4.3 1,034,283 - Psest_0953 0.14 -1.4
Or see this region's nucleotide sequence