Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0061 and Psest_0062 are separated by 175 nucleotides Psest_0062 and Psest_0063 overlap by 4 nucleotides Psest_0063 and Psest_0064 are separated by 171 nucleotides
Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786
_0061
Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771
_0062
Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979
_0063
Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053
_0064
Position (kb) 62
63
64 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 61.896 kb on + strand at 61.897 kb on - strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.980 kb on + strand at 61.980 kb on + strand at 61.981 kb on - strand at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.248 kb on + strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.684 kb on - strand, within Psest_0062 at 62.992 kb on - strand, within Psest_0063 at 62.992 kb on - strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.564 kb on + strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.787 kb on - strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.811 kb on - strand, within Psest_0063 at 63.811 kb on - strand, within Psest_0063 at 63.826 kb on - strand, within Psest_0063 at 63.826 kb on - strand, within Psest_0063 at 64.086 kb on + strand at 64.150 kb on + strand at 64.150 kb on + strand at 64.209 kb on + strand at 64.210 kb on - strand at 64.210 kb on - strand at 64.210 kb on - strand at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 61,896 + -0.1 61,897 - -0.1 61,900 + -1.6 61,900 + -0.5 61,900 + +1.9 61,901 - -0.3 61,901 - -0.3 61,901 - +0.5 61,901 - -0.2 61,901 - -1.7 61,980 + +0.6 61,980 + +0.3 61,981 - +0.7 62,245 + Psest_0062 0.35 -2.5 62,245 + Psest_0062 0.35 -4.1 62,245 + Psest_0062 0.35 -2.2 62,245 + Psest_0062 0.35 -2.1 62,246 - Psest_0062 0.35 +0.3 62,246 - Psest_0062 0.35 -2.0 62,246 - Psest_0062 0.35 -0.5 62,246 - Psest_0062 0.35 -0.5 62,246 - Psest_0062 0.35 -1.8 62,248 + Psest_0062 0.35 -3.8 62,249 - Psest_0062 0.35 -1.7 62,249 - Psest_0062 0.35 -1.5 62,249 - Psest_0062 0.35 -1.9 62,464 + Psest_0062 0.62 -5.4 62,464 + Psest_0062 0.62 -2.2 62,464 + Psest_0062 0.62 -4.4 62,465 - Psest_0062 0.62 -2.6 62,465 - Psest_0062 0.62 -2.0 62,465 - Psest_0062 0.62 -3.0 62,465 - Psest_0062 0.62 -3.0 62,465 - Psest_0062 0.62 -2.1 62,465 - Psest_0062 0.62 -0.7 62,465 - Psest_0062 0.62 -0.7 62,465 - Psest_0062 0.62 -2.3 62,465 - Psest_0062 0.62 -3.0 62,465 - Psest_0062 0.62 -0.1 62,465 - Psest_0062 0.62 -3.1 62,465 - Psest_0062 0.62 -0.1 62,684 - Psest_0062 0.89 -4.4 62,992 - Psest_0063 0.18 -2.5 62,992 - Psest_0063 0.18 -3.6 63,405 + Psest_0063 0.53 +0.9 63,405 + Psest_0063 0.53 -1.1 63,406 - Psest_0063 0.53 -2.1 63,406 - Psest_0063 0.53 -2.3 63,406 - Psest_0063 0.53 -4.0 63,406 - Psest_0063 0.53 -2.3 63,406 - Psest_0063 0.53 -0.1 63,406 - Psest_0063 0.53 -0.1 63,406 - Psest_0063 0.53 -0.7 63,406 - Psest_0063 0.53 -1.7 63,564 + Psest_0063 0.66 -1.9 63,565 - Psest_0063 0.66 -1.8 63,565 - Psest_0063 0.66 -3.0 63,565 - Psest_0063 0.66 -0.9 63,565 - Psest_0063 0.66 -1.7 63,565 - Psest_0063 0.66 -2.5 63,741 + Psest_0063 0.80 -2.0 63,741 + Psest_0063 0.80 -0.5 63,741 + Psest_0063 0.80 +0.9 63,741 + Psest_0063 0.80 -0.9 63,741 + Psest_0063 0.80 -2.4 63,742 - Psest_0063 0.80 -3.7 63,742 - Psest_0063 0.80 -1.6 63,742 - Psest_0063 0.80 +1.9 63,742 - Psest_0063 0.80 -1.0 63,787 - Psest_0063 0.84 -0.9 63,810 + Psest_0063 0.86 +0.9 63,810 + Psest_0063 0.86 -0.1 63,810 + Psest_0063 0.86 -2.8 63,810 + Psest_0063 0.86 -4.5 63,810 + Psest_0063 0.86 -0.5 63,810 + Psest_0063 0.86 +0.9 63,810 + Psest_0063 0.86 -1.6 63,810 + Psest_0063 0.86 -2.0 63,811 - Psest_0063 0.86 -0.5 63,811 - Psest_0063 0.86 -1.3 63,826 - Psest_0063 0.87 -4.3 63,826 - Psest_0063 0.87 -3.6 64,086 + +1.9 64,150 + +0.9 64,150 + +0.3 64,209 + +0.5 64,210 - +0.2 64,210 - +0.4 64,210 - -0.7 64,829 + Psest_0064 0.75 +2.3 64,829 + Psest_0064 0.75 -0.7 64,829 + Psest_0064 0.75 +0.3 64,829 + Psest_0064 0.75 +0.3 64,830 - Psest_0064 0.75 +0.9 64,830 - Psest_0064 0.75 -0.1 64,830 - Psest_0064 0.75 +1.9 64,830 - Psest_0064 0.75 +0.9 64,830 - Psest_0064 0.75 +1.1
Or see this region's nucleotide sequence