Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0412 and Psest_0413 overlap by 8 nucleotides Psest_0413 and Psest_0414 overlap by 8 nucleotides Psest_0414 and Psest_0415 are separated by 1 nucleotides Psest_0415 and Psest_0416 are separated by 85 nucleotides
Psest_0412: Psest_0412 - 8-amino-7-oxononanoate synthase, at 447,762 to 448,940
_0412
Psest_0413: Psest_0413 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 448,933 to 449,658
_0413
Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445
_0414
Psest_0415: Psest_0415 - dethiobiotin synthase, at 450,447 to 451,133
_0415
Psest_0416: Psest_0416 - hypothetical protein, at 451,219 to 451,467
_0416
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 448.859 kb on + strand at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.461 kb on + strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.721 kb on - strand at 449.721 kb on - strand at 449.721 kb on - strand at 449.993 kb on + strand, within Psest_0414 at 449.993 kb on + strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.420 kb on - strand at 450.420 kb on - strand at 450.557 kb on - strand, within Psest_0415 at 450.794 kb on - strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.838 kb on - strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 451.233 kb on + strand at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 448,859 + -0.8 449,196 + Psest_0413 0.36 -1.2 449,196 + Psest_0413 0.36 -1.7 449,196 + Psest_0413 0.36 +1.8 449,196 + Psest_0413 0.36 -0.4 449,197 - Psest_0413 0.36 -1.2 449,197 - Psest_0413 0.36 +0.1 449,197 - Psest_0413 0.36 -0.5 449,197 - Psest_0413 0.36 -1.5 449,197 - Psest_0413 0.36 -1.3 449,341 + Psest_0413 0.56 -0.2 449,341 + Psest_0413 0.56 +1.8 449,341 + Psest_0413 0.56 -1.2 449,342 - Psest_0413 0.56 -3.1 449,342 - Psest_0413 0.56 +0.0 449,461 + Psest_0413 0.73 -0.8 449,462 - Psest_0413 0.73 -1.2 449,462 - Psest_0413 0.73 -0.5 449,721 - -1.4 449,721 - -1.5 449,721 - -0.5 449,993 + Psest_0414 0.43 -0.5 449,993 + Psest_0414 0.43 -1.4 449,994 - Psest_0414 0.43 -0.6 449,994 - Psest_0414 0.43 +0.1 449,994 - Psest_0414 0.43 -2.0 450,182 + Psest_0414 0.67 -0.3 450,182 + Psest_0414 0.67 -1.3 450,182 + Psest_0414 0.67 -2.4 450,182 + Psest_0414 0.67 -1.4 450,183 - Psest_0414 0.67 -0.5 450,183 - Psest_0414 0.67 -0.2 450,183 - Psest_0414 0.67 -0.9 450,183 - Psest_0414 0.67 -0.7 450,407 + -0.6 450,407 + -1.2 450,407 + -0.4 450,407 + -0.2 450,408 - -1.4 450,408 - +0.4 450,408 - -1.3 450,408 - +0.6 450,420 - -2.6 450,420 - -1.8 450,557 - Psest_0415 0.16 -1.1 450,794 - Psest_0415 0.51 -1.4 450,837 + Psest_0415 0.57 -0.1 450,837 + Psest_0415 0.57 -1.2 450,837 + Psest_0415 0.57 -0.9 450,838 - Psest_0415 0.57 -1.6 450,922 + Psest_0415 0.69 -0.7 450,922 + Psest_0415 0.69 -0.2 450,923 - Psest_0415 0.69 -1.2 450,923 - Psest_0415 0.69 +0.2 450,923 - Psest_0415 0.69 -0.6 450,923 - Psest_0415 0.69 -3.4 451,233 + +0.4 451,261 + Psest_0416 0.17 -0.1 451,261 + Psest_0416 0.17 +1.4 451,261 + Psest_0416 0.17 +0.3 451,262 - Psest_0416 0.17 +0.0 451,262 - Psest_0416 0.17 +0.1 451,262 - Psest_0416 0.17 -0.4 451,262 - Psest_0416 0.17 +0.6 451,262 - Psest_0416 0.17 -0.7 451,262 - Psest_0416 0.17 +0.2 451,262 - Psest_0416 0.17 -0.0
Or see this region's nucleotide sequence