Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0411 and Psest_0412 are separated by 107 nucleotides Psest_0412 and Psest_0413 overlap by 8 nucleotides Psest_0413 and Psest_0414 overlap by 8 nucleotides
Psest_0411: Psest_0411 - biotin synthetase, at 446,599 to 447,654
_0411
Psest_0412: Psest_0412 - 8-amino-7-oxononanoate synthase, at 447,762 to 448,940
_0412
Psest_0413: Psest_0413 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 448,933 to 449,658
_0413
Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445
_0414
Position (kb)
447
448
449 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.792 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.950 kb on + strand, within Psest_0411 at 446.951 kb on - strand, within Psest_0411 at 446.951 kb on - strand, within Psest_0411 at 447.062 kb on - strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.081 kb on + strand, within Psest_0411 at 447.094 kb on + strand, within Psest_0411 at 447.094 kb on + strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.097 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.638 kb on - strand at 447.640 kb on - strand at 447.814 kb on - strand at 447.900 kb on + strand, within Psest_0412 at 447.901 kb on - strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.082 kb on + strand, within Psest_0412 at 448.083 kb on - strand, within Psest_0412 at 448.282 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.613 kb on + strand, within Psest_0412 at 448.614 kb on - strand, within Psest_0412 at 448.859 kb on + strand at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.461 kb on + strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.721 kb on - strand at 449.721 kb on - strand at 449.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 446,791 + Psest_0411 0.18 -1.6 446,791 + Psest_0411 0.18 -1.7 446,791 + Psest_0411 0.18 -2.0 446,791 + Psest_0411 0.18 -1.0 446,791 + Psest_0411 0.18 -1.4 446,791 + Psest_0411 0.18 -2.2 446,792 - Psest_0411 0.18 -1.0 446,813 - Psest_0411 0.20 -1.9 446,813 - Psest_0411 0.20 -0.8 446,813 - Psest_0411 0.20 -1.4 446,950 + Psest_0411 0.33 -2.8 446,951 - Psest_0411 0.33 -2.2 446,951 - Psest_0411 0.33 -3.2 447,062 - Psest_0411 0.44 -3.1 447,064 + Psest_0411 0.44 -3.5 447,064 + Psest_0411 0.44 -2.1 447,064 + Psest_0411 0.44 -2.6 447,064 + Psest_0411 0.44 -2.5 447,064 + Psest_0411 0.44 -1.0 447,065 - Psest_0411 0.44 -3.7 447,065 - Psest_0411 0.44 -2.6 447,065 - Psest_0411 0.44 -0.5 447,065 - Psest_0411 0.44 -1.9 447,081 + Psest_0411 0.46 -2.2 447,094 + Psest_0411 0.47 -2.2 447,094 + Psest_0411 0.47 -1.3 447,095 - Psest_0411 0.47 -0.5 447,095 - Psest_0411 0.47 -2.1 447,095 - Psest_0411 0.47 -2.3 447,097 + Psest_0411 0.47 -4.0 447,124 + Psest_0411 0.50 -3.4 447,124 + Psest_0411 0.50 -1.5 447,124 + Psest_0411 0.50 -1.7 447,152 - Psest_0411 0.52 -2.0 447,152 - Psest_0411 0.52 -1.2 447,152 - Psest_0411 0.52 -2.3 447,152 - Psest_0411 0.52 -0.2 447,152 - Psest_0411 0.52 +0.8 447,152 - Psest_0411 0.52 -3.2 447,152 - Psest_0411 0.52 -1.5 447,637 + +1.8 447,637 + -0.4 447,637 + -0.6 447,637 + +0.1 447,637 + -0.3 447,637 + +0.2 447,638 - -0.5 447,640 - -1.2 447,814 - -2.4 447,900 + Psest_0412 0.12 -0.2 447,901 - Psest_0412 0.12 -1.0 448,080 + Psest_0412 0.27 -0.7 448,080 + Psest_0412 0.27 -0.5 448,080 + Psest_0412 0.27 -0.4 448,080 + Psest_0412 0.27 -2.0 448,080 + Psest_0412 0.27 -0.9 448,080 + Psest_0412 0.27 -1.6 448,080 + Psest_0412 0.27 -1.9 448,081 - Psest_0412 0.27 +0.2 448,081 - Psest_0412 0.27 -0.5 448,081 - Psest_0412 0.27 -1.5 448,081 - Psest_0412 0.27 -1.1 448,082 + Psest_0412 0.27 -1.2 448,083 - Psest_0412 0.27 -2.2 448,282 + Psest_0412 0.44 -0.0 448,545 + Psest_0412 0.66 -1.5 448,545 + Psest_0412 0.66 -1.2 448,545 + Psest_0412 0.66 -3.5 448,545 + Psest_0412 0.66 -0.6 448,546 - Psest_0412 0.66 -0.2 448,546 - Psest_0412 0.66 -1.5 448,546 - Psest_0412 0.66 -1.7 448,551 + Psest_0412 0.67 -0.8 448,551 + Psest_0412 0.67 -0.1 448,551 + Psest_0412 0.67 -3.4 448,552 - Psest_0412 0.67 -1.6 448,552 - Psest_0412 0.67 -0.5 448,552 - Psest_0412 0.67 -1.6 448,552 - Psest_0412 0.67 -0.2 448,552 - Psest_0412 0.67 -0.0 448,552 - Psest_0412 0.67 -1.7 448,552 - Psest_0412 0.67 +0.8 448,552 - Psest_0412 0.67 -0.7 448,552 - Psest_0412 0.67 -2.0 448,552 - Psest_0412 0.67 -1.3 448,552 - Psest_0412 0.67 -0.7 448,552 - Psest_0412 0.67 -0.9 448,552 - Psest_0412 0.67 -0.6 448,552 - Psest_0412 0.67 -1.3 448,552 - Psest_0412 0.67 -1.6 448,613 + Psest_0412 0.72 -1.2 448,614 - Psest_0412 0.72 -0.8 448,859 + -0.8 449,196 + Psest_0413 0.36 -1.2 449,196 + Psest_0413 0.36 -1.7 449,196 + Psest_0413 0.36 +1.8 449,196 + Psest_0413 0.36 -0.4 449,197 - Psest_0413 0.36 -1.2 449,197 - Psest_0413 0.36 +0.1 449,197 - Psest_0413 0.36 -0.5 449,197 - Psest_0413 0.36 -1.5 449,197 - Psest_0413 0.36 -1.3 449,341 + Psest_0413 0.56 -0.2 449,341 + Psest_0413 0.56 +1.8 449,341 + Psest_0413 0.56 -1.2 449,342 - Psest_0413 0.56 -3.1 449,342 - Psest_0413 0.56 +0.0 449,461 + Psest_0413 0.73 -0.8 449,462 - Psest_0413 0.73 -1.2 449,462 - Psest_0413 0.73 -0.5 449,721 - -1.4 449,721 - -1.5 449,721 - -0.5
Or see this region's nucleotide sequence