Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0312 and Psest_0313 are separated by 236 nucleotides Psest_0313 and Psest_0314 are separated by 86 nucleotides
Psest_0312: Psest_0312 - Superfamily II DNA and RNA helicases, at 334,149 to 335,966
_0312
Psest_0313: Psest_0313 - 5,10-methylenetetrahydrofolate reductase, prokaryotic form, at 336,203 to 337,132
_0313
Psest_0314: Psest_0314 - adenosylhomocysteinase, at 337,219 to 338,625
_0314
Position (kb) 336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 335.212 kb on - strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.662 kb on + strand, within Psest_0312 at 335.663 kb on - strand, within Psest_0312 at 335.663 kb on - strand, within Psest_0312 at 335.970 kb on + strand at 335.970 kb on + strand at 335.970 kb on + strand at 335.971 kb on - strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.061 kb on + strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.062 kb on - strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.065 kb on + strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.066 kb on - strand at 336.067 kb on + strand at 336.067 kb on + strand at 336.067 kb on + strand at 336.068 kb on - strand at 336.068 kb on - strand at 336.086 kb on + strand at 336.108 kb on + strand at 336.108 kb on + strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.109 kb on - strand at 336.110 kb on + strand at 336.110 kb on + strand at 336.110 kb on + strand at 336.111 kb on - strand at 336.178 kb on + strand at 336.179 kb on - strand at 336.264 kb on + strand at 336.438 kb on + strand, within Psest_0313 at 336.439 kb on - strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.510 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.516 kb on + strand, within Psest_0313 at 336.517 kb on - strand, within Psest_0313 at 336.537 kb on + strand, within Psest_0313 at 336.538 kb on - strand, within Psest_0313 at 336.538 kb on - strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.786 kb on + strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.787 kb on - strand, within Psest_0313 at 336.860 kb on + strand, within Psest_0313 at 336.924 kb on + strand, within Psest_0313 at 336.925 kb on - strand, within Psest_0313 at 337.100 kb on + strand at 337.101 kb on - strand at 337.127 kb on + strand at 337.168 kb on - strand at 337.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 335,212 - Psest_0312 0.58 -0.7 335,662 + Psest_0312 0.83 -0.3 335,662 + Psest_0312 0.83 -1.7 335,662 + Psest_0312 0.83 +0.7 335,663 - Psest_0312 0.83 +0.3 335,663 - Psest_0312 0.83 -1.3 335,970 + +1.8 335,970 + +0.8 335,970 + +0.3 335,971 - +0.3 336,061 + -1.0 336,061 + +0.5 336,061 + +1.8 336,061 + -1.7 336,061 + +1.8 336,062 - +1.8 336,062 - -1.0 336,062 - -0.5 336,062 - -0.8 336,065 + -0.2 336,065 + -0.5 336,065 + +0.5 336,065 + -0.2 336,065 + +0.3 336,065 + +0.8 336,065 + -0.5 336,066 - +1.0 336,066 - -1.7 336,066 - +0.8 336,066 - +0.8 336,066 - +0.8 336,066 - +0.4 336,066 - +0.4 336,066 - -0.4 336,066 - +0.0 336,066 - +1.4 336,066 - -0.5 336,066 - -1.5 336,066 - -0.7 336,066 - +0.3 336,066 - +0.8 336,066 - +0.0 336,066 - -0.2 336,067 + -1.3 336,067 + -0.6 336,067 + -0.7 336,068 - +1.6 336,068 - -0.7 336,086 + +0.6 336,108 + -1.0 336,108 + -1.6 336,109 - -0.5 336,109 - +0.0 336,109 - -0.7 336,109 - +1.3 336,110 + -0.5 336,110 + -0.2 336,110 + +0.3 336,111 - -0.5 336,178 + -0.3 336,179 - +0.8 336,264 + -1.3 336,438 + Psest_0313 0.25 -0.7 336,439 - Psest_0313 0.25 +0.3 336,510 + Psest_0313 0.33 -0.7 336,510 + Psest_0313 0.33 -3.1 336,510 + Psest_0313 0.33 -1.0 336,516 + Psest_0313 0.34 -1.6 336,516 + Psest_0313 0.34 -0.5 336,516 + Psest_0313 0.34 -0.2 336,517 - Psest_0313 0.34 +1.8 336,537 + Psest_0313 0.36 -1.2 336,538 - Psest_0313 0.36 -0.2 336,538 - Psest_0313 0.36 -0.7 336,786 + Psest_0313 0.63 -1.6 336,786 + Psest_0313 0.63 -2.2 336,786 + Psest_0313 0.63 -1.2 336,786 + Psest_0313 0.63 -0.5 336,786 + Psest_0313 0.63 -0.5 336,787 - Psest_0313 0.63 -0.5 336,787 - Psest_0313 0.63 -0.7 336,787 - Psest_0313 0.63 -0.5 336,787 - Psest_0313 0.63 -0.7 336,787 - Psest_0313 0.63 +0.3 336,787 - Psest_0313 0.63 +0.3 336,860 + Psest_0313 0.71 -1.0 336,924 + Psest_0313 0.78 -0.7 336,925 - Psest_0313 0.78 -0.5 337,100 + -2.3 337,101 - +0.5 337,127 + +0.3 337,168 - -0.6 337,168 - -0.2
Or see this region's nucleotide sequence