Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2944 and Psest_2945 are separated by 158 nucleotides Psest_2945 and Psest_2946 are separated by 95 nucleotides Psest_2946 and Psest_2947 overlap by 4 nucleotides
Psest_2944: Psest_2944 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase, at 3,141,414 to 3,142,355
_2944
Psest_2945: Psest_2945 - septum site-determining protein MinC, at 3,142,514 to 3,143,239
_2945
Psest_2946: Psest_2946 - septum site-determining protein MinD, at 3,143,335 to 3,144,150
_2946
Psest_2947: Psest_2947 - cell division topological specificity factor MinE, at 3,144,147 to 3,144,404
_2947
Position (kb)
3142
3143
3144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3141.755 kb on - strand, within Psest_2944 at 3141.755 kb on - strand, within Psest_2944 at 3141.851 kb on + strand, within Psest_2944 at 3141.910 kb on + strand, within Psest_2944 at 3141.910 kb on + strand, within Psest_2944 at 3141.910 kb on + strand, within Psest_2944 at 3141.911 kb on - strand, within Psest_2944 at 3141.911 kb on - strand, within Psest_2944 at 3141.911 kb on - strand, within Psest_2944 at 3141.991 kb on - strand, within Psest_2944 at 3142.330 kb on + strand at 3142.331 kb on - strand at 3142.359 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.481 kb on + strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.482 kb on - strand at 3142.497 kb on - strand at 3143.039 kb on + strand, within Psest_2945 at 3143.040 kb on - strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.071 kb on + strand, within Psest_2945 at 3143.244 kb on + strand at 3143.379 kb on + strand at 3143.379 kb on + strand at 3143.380 kb on - strand at 3143.506 kb on + strand, within Psest_2946 at 3143.507 kb on - strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.678 kb on - strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.732 kb on - strand, within Psest_2946 at 3143.756 kb on + strand, within Psest_2946 at 3143.867 kb on + strand, within Psest_2946 at 3143.878 kb on + strand, within Psest_2946 at 3143.879 kb on - strand, within Psest_2946 at 3144.047 kb on - strand, within Psest_2946
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 3,141,755 - Psest_2944 0.36 -0.2 3,141,755 - Psest_2944 0.36 +0.2 3,141,851 + Psest_2944 0.46 +1.4 3,141,910 + Psest_2944 0.53 -0.8 3,141,910 + Psest_2944 0.53 +0.4 3,141,910 + Psest_2944 0.53 +0.1 3,141,911 - Psest_2944 0.53 +0.1 3,141,911 - Psest_2944 0.53 -0.2 3,141,911 - Psest_2944 0.53 -0.1 3,141,991 - Psest_2944 0.61 +3.0 3,142,330 + -0.6 3,142,331 - -0.3 3,142,359 + +0.1 3,142,481 + +0.3 3,142,481 + -0.7 3,142,481 + -0.5 3,142,481 + -0.4 3,142,481 + -1.0 3,142,481 + -1.0 3,142,481 + -0.1 3,142,481 + -0.3 3,142,481 + +0.4 3,142,482 - -0.7 3,142,482 - -0.0 3,142,482 - +0.2 3,142,482 - -0.9 3,142,482 - -0.4 3,142,482 - +2.1 3,142,482 - +0.0 3,142,482 - -1.3 3,142,497 - -0.2 3,143,039 + Psest_2945 0.72 -0.9 3,143,040 - Psest_2945 0.72 -1.8 3,143,071 + Psest_2945 0.77 -0.9 3,143,071 + Psest_2945 0.77 +0.8 3,143,071 + Psest_2945 0.77 -0.6 3,143,244 + -0.7 3,143,379 + +0.4 3,143,379 + -1.8 3,143,380 - -1.9 3,143,506 + Psest_2946 0.21 -1.6 3,143,507 - Psest_2946 0.21 -1.8 3,143,677 + Psest_2946 0.42 -2.2 3,143,677 + Psest_2946 0.42 -0.8 3,143,677 + Psest_2946 0.42 -1.5 3,143,678 - Psest_2946 0.42 -0.9 3,143,731 + Psest_2946 0.49 -2.6 3,143,731 + Psest_2946 0.49 -0.9 3,143,731 + Psest_2946 0.49 -0.3 3,143,731 + Psest_2946 0.49 -1.7 3,143,731 + Psest_2946 0.49 +0.4 3,143,731 + Psest_2946 0.49 -1.2 3,143,731 + Psest_2946 0.49 -2.2 3,143,732 - Psest_2946 0.49 -0.9 3,143,756 + Psest_2946 0.52 -0.9 3,143,867 + Psest_2946 0.65 -0.2 3,143,878 + Psest_2946 0.67 -1.1 3,143,879 - Psest_2946 0.67 -0.9 3,144,047 - Psest_2946 0.87 -2.9
Or see this region's nucleotide sequence