Experiment: Def Fusidic 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0191 and Psest_0192 overlap by 4 nucleotides Psest_0192 and Psest_0193 are separated by 307 nucleotides Psest_0193 and Psest_0194 are separated by 178 nucleotides
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Psest_0192: Psest_0192 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 188,256 to 189,602
_0192
Psest_0193: Psest_0193 - conserved hypothetical protein, at 189,910 to 190,341
_0193
Psest_0194: Psest_0194 - hypothetical protein, at 190,520 to 190,756
_0194
Position (kb) 188
189
190 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 187.799 kb on + strand, within Psest_0191 at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.090 kb on - strand at 188.259 kb on + strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.686 kb on + strand, within Psest_0192 at 188.687 kb on - strand, within Psest_0192 at 188.800 kb on + strand, within Psest_0192 at 188.982 kb on + strand, within Psest_0192 at 189.286 kb on + strand, within Psest_0192 at 189.287 kb on - strand, within Psest_0192 at 189.287 kb on - strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.503 kb on + strand at 189.503 kb on + strand at 189.503 kb on + strand at 189.503 kb on + strand at 189.505 kb on + strand at 189.505 kb on + strand at 189.536 kb on + strand at 189.537 kb on - strand at 189.650 kb on + strand at 189.788 kb on + strand at 189.789 kb on - strand at 189.974 kb on - strand, within Psest_0193 at 190.138 kb on + strand, within Psest_0193 at 190.474 kb on - strand at 190.505 kb on + strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Fusidic 0.032 mg/ml remove 187,799 + Psest_0191 0.74 -2.5 188,089 + +1.2 188,089 + -0.5 188,089 + +1.3 188,089 + -0.3 188,089 + -0.1 188,089 + +0.3 188,090 - -0.8 188,259 + -0.1 188,260 - -0.3 188,260 - +0.1 188,260 - -0.2 188,530 + Psest_0192 0.20 -0.3 188,530 + Psest_0192 0.20 -0.6 188,530 + Psest_0192 0.20 -0.6 188,530 + Psest_0192 0.20 -1.0 188,531 - Psest_0192 0.20 +0.3 188,531 - Psest_0192 0.20 +0.2 188,531 - Psest_0192 0.20 +0.9 188,686 + Psest_0192 0.32 -0.9 188,687 - Psest_0192 0.32 -1.3 188,800 + Psest_0192 0.40 -0.3 188,982 + Psest_0192 0.54 -0.8 189,286 + Psest_0192 0.76 -2.1 189,287 - Psest_0192 0.77 +0.3 189,287 - Psest_0192 0.77 -1.3 189,393 + Psest_0192 0.84 -2.1 189,393 + Psest_0192 0.84 -2.2 189,393 + Psest_0192 0.84 -0.5 189,393 + Psest_0192 0.84 -3.1 189,394 - Psest_0192 0.84 -0.5 189,394 - Psest_0192 0.84 -0.1 189,394 - Psest_0192 0.84 +0.3 189,394 - Psest_0192 0.84 +0.1 189,394 - Psest_0192 0.84 -0.5 189,394 - Psest_0192 0.84 -1.8 189,394 - Psest_0192 0.84 -0.8 189,394 - Psest_0192 0.84 -0.0 189,503 + -1.0 189,503 + +0.1 189,503 + -5.3 189,503 + -2.0 189,505 + -1.8 189,505 + -2.5 189,536 + -0.7 189,537 - +1.9 189,650 + -1.9 189,788 + -0.3 189,789 - +0.5 189,974 - Psest_0193 0.15 -1.7 190,138 + Psest_0193 0.53 +0.2 190,474 - +0.5 190,505 + -0.1 190,506 - -1.1 190,506 - -0.9 190,506 - -0.4 190,506 - +0.3 190,506 - -0.5 190,506 - -0.2 190,506 - -0.3
Or see this region's nucleotide sequence