Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0090

Experiment: Def 5-Hydroxymethylfurfural 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0088 and Psest_0089 overlap by 10 nucleotidesPsest_0089 and Psest_0090 are separated by 98 nucleotidesPsest_0090 and Psest_0091 overlap by 11 nucleotidesPsest_0091 and Psest_0092 are separated by 11 nucleotides Psest_0088: Psest_0088 - phosphonate metabolism protein PhnP, at 89,559 to 90,308 _0088 Psest_0089: Psest_0089 - phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN, at 90,299 to 90,853 _0089 Psest_0090: Psest_0090 - phosphonate metabolism protein PhnM, at 90,952 to 92,097 _0090 Psest_0091: Psest_0091 - phosphonate C-P lyase system protein PhnL, at 92,087 to 92,791 _0091 Psest_0092: Psest_0092 - phosphonate C-P lyase system protein PhnK, at 92,803 to 93,615 _0092 Position (kb) 90 91 92 93Strain fitness (log2 ratio) -2 -1 0 1 2 3at 89.983 kb on + strand, within Psest_0088at 89.983 kb on + strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 89.984 kb on - strand, within Psest_0088at 90.259 kb on + strandat 90.259 kb on + strandat 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.384 kb on + strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.385 kb on - strand, within Psest_0089at 90.549 kb on + strand, within Psest_0089at 90.621 kb on + strand, within Psest_0089at 90.835 kb on - strandat 90.835 kb on - strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.898 kb on + strandat 90.899 kb on - strandat 90.899 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.010 kb on - strandat 91.182 kb on - strand, within Psest_0090at 91.374 kb on + strand, within Psest_0090at 91.529 kb on + strand, within Psest_0090at 91.529 kb on + strand, within Psest_0090at 91.529 kb on + strand, within Psest_0090at 91.530 kb on - strand, within Psest_0090at 91.530 kb on - strand, within Psest_0090at 91.530 kb on - strand, within Psest_0090at 91.530 kb on - strand, within Psest_0090at 91.530 kb on - strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.541 kb on + strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.542 kb on - strand, within Psest_0090at 91.544 kb on + strand, within Psest_0090at 91.545 kb on - strand, within Psest_0090at 91.554 kb on - strand, within Psest_0090at 92.080 kb on + strandat 92.080 kb on + strandat 92.080 kb on + strandat 92.081 kb on - strandat 92.081 kb on - strandat 92.503 kb on - strand, within Psest_0091at 92.531 kb on + strand, within Psest_0091at 92.531 kb on + strand, within Psest_0091at 92.532 kb on - strand, within Psest_0091at 92.532 kb on - strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.610 kb on + strand, within Psest_0091at 92.611 kb on - strand, within Psest_0091at 92.611 kb on - strand, within Psest_0091at 92.611 kb on - strand, within Psest_0091at 92.611 kb on - strand, within Psest_0091at 92.620 kb on - strand, within Psest_0091at 92.838 kb on + strandat 92.839 kb on - strandat 92.839 kb on - strandat 92.840 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def 5-Hydroxymethylfurfural 5 mM
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89,983 + Psest_0088 0.57 +0.2
89,983 + Psest_0088 0.57 -0.4
89,984 - Psest_0088 0.57 +0.6
89,984 - Psest_0088 0.57 +1.1
89,984 - Psest_0088 0.57 -0.4
89,984 - Psest_0088 0.57 +0.8
90,259 + +0.4
90,259 + +0.5
90,384 + Psest_0089 0.15 -1.4
90,384 + Psest_0089 0.15 +0.5
90,384 + Psest_0089 0.15 +0.5
90,384 + Psest_0089 0.15 +1.2
90,384 + Psest_0089 0.15 -0.4
90,384 + Psest_0089 0.15 +0.2
90,385 - Psest_0089 0.15 -0.8
90,385 - Psest_0089 0.15 -0.8
90,385 - Psest_0089 0.15 +0.5
90,385 - Psest_0089 0.15 +0.9
90,385 - Psest_0089 0.15 -0.4
90,385 - Psest_0089 0.15 -0.8
90,385 - Psest_0089 0.15 +0.2
90,385 - Psest_0089 0.15 +1.2
90,549 + Psest_0089 0.45 +1.4
90,621 + Psest_0089 0.58 +0.6
90,835 - +1.4
90,835 - -0.4
90,898 + -0.4
90,898 + +0.7
90,898 + -1.6
90,898 + -0.1
90,898 + +1.7
90,898 + +0.4
90,898 + +1.2
90,898 + +0.6
90,899 - -0.4
90,899 - +0.8
91,010 - +0.9
91,010 - +2.2
91,010 - -1.1
91,010 - +2.5
91,010 - +0.2
91,182 - Psest_0090 0.20 +0.2
91,374 + Psest_0090 0.37 +2.8
91,529 + Psest_0090 0.50 +1.2
91,529 + Psest_0090 0.50 +1.6
91,529 + Psest_0090 0.50 +1.2
91,530 - Psest_0090 0.50 +0.2
91,530 - Psest_0090 0.50 -0.7
91,530 - Psest_0090 0.50 +1.8
91,530 - Psest_0090 0.50 -0.0
91,530 - Psest_0090 0.50 +0.2
91,541 + Psest_0090 0.51 +0.2
91,541 + Psest_0090 0.51 +0.4
91,541 + Psest_0090 0.51 +0.9
91,541 + Psest_0090 0.51 +0.4
91,541 + Psest_0090 0.51 -1.1
91,541 + Psest_0090 0.51 +0.2
91,541 + Psest_0090 0.51 +3.0
91,541 + Psest_0090 0.51 +1.6
91,541 + Psest_0090 0.51 +1.2
91,541 + Psest_0090 0.51 -0.6
91,541 + Psest_0090 0.51 +0.8
91,541 + Psest_0090 0.51 +1.5
91,541 + Psest_0090 0.51 +0.3
91,541 + Psest_0090 0.51 +0.2
91,541 + Psest_0090 0.51 +3.2
91,541 + Psest_0090 0.51 -1.2
91,541 + Psest_0090 0.51 +2.2
91,541 + Psest_0090 0.51 +0.2
91,541 + Psest_0090 0.51 +0.2
91,541 + Psest_0090 0.51 +0.4
91,541 + Psest_0090 0.51 +1.0
91,541 + Psest_0090 0.51 +2.2
91,541 + Psest_0090 0.51 +1.2
91,541 + Psest_0090 0.51 -1.6
91,541 + Psest_0090 0.51 -0.1
91,542 - Psest_0090 0.51 +0.7
91,542 - Psest_0090 0.51 -0.4
91,542 - Psest_0090 0.51 +2.2
91,542 - Psest_0090 0.51 +1.4
91,542 - Psest_0090 0.51 -0.4
91,542 - Psest_0090 0.51 -0.8
91,542 - Psest_0090 0.51 +0.9
91,542 - Psest_0090 0.51 -0.4
91,542 - Psest_0090 0.51 +0.5
91,542 - Psest_0090 0.51 +0.2
91,542 - Psest_0090 0.51 +1.2
91,542 - Psest_0090 0.51 +2.2
91,542 - Psest_0090 0.51 -0.1
91,542 - Psest_0090 0.51 +2.2
91,542 - Psest_0090 0.51 +1.2
91,542 - Psest_0090 0.51 +1.8
91,542 - Psest_0090 0.51 +2.8
91,542 - Psest_0090 0.51 +1.2
91,542 - Psest_0090 0.51 -0.1
91,542 - Psest_0090 0.51 +1.3
91,542 - Psest_0090 0.51 -0.0
91,542 - Psest_0090 0.51 +2.2
91,542 - Psest_0090 0.51 -0.9
91,542 - Psest_0090 0.51 +0.6
91,542 - Psest_0090 0.51 -0.4
91,542 - Psest_0090 0.51 +0.2
91,542 - Psest_0090 0.51 -1.1
91,542 - Psest_0090 0.51 -1.1
91,542 - Psest_0090 0.51 +1.2
91,542 - Psest_0090 0.51 +1.0
91,542 - Psest_0090 0.51 -0.4
91,544 + Psest_0090 0.52 -1.2
91,545 - Psest_0090 0.52 -0.2
91,554 - Psest_0090 0.53 +0.9
92,080 + +1.5
92,080 + +2.2
92,080 + +0.2
92,081 - -0.1
92,081 - +2.2
92,503 - Psest_0091 0.59 +1.8
92,531 + Psest_0091 0.63 -1.4
92,531 + Psest_0091 0.63 +1.2
92,532 - Psest_0091 0.63 -2.1
92,532 - Psest_0091 0.63 +0.5
92,610 + Psest_0091 0.74 +0.6
92,610 + Psest_0091 0.74 +0.8
92,610 + Psest_0091 0.74 +1.2
92,610 + Psest_0091 0.74 -1.1
92,610 + Psest_0091 0.74 +3.4
92,610 + Psest_0091 0.74 +0.2
92,611 - Psest_0091 0.74 +0.8
92,611 - Psest_0091 0.74 -0.4
92,611 - Psest_0091 0.74 +0.2
92,611 - Psest_0091 0.74 +1.2
92,620 - Psest_0091 0.76 +1.8
92,838 + +0.2
92,839 - +0.2
92,839 - -0.8
92,840 + +0.0

Or see this region's nucleotide sequence