Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0182

Experiment: Def 5-Hydroxymethylfurfural 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0181 and Psest_0182 are separated by 52 nucleotidesPsest_0182 and Psest_0183 are separated by 59 nucleotides Psest_0181: Psest_0181 - glycine cleavage system T protein, at 175,363 to 176,445 _0181 Psest_0182: Psest_0182 - glycine cleavage system H protein, at 176,498 to 176,887 _0182 Psest_0183: Psest_0183 - glycine dehydrogenase (decarboxylating), at 176,947 to 179,823 _0183 Position (kb) 176 177Strain fitness (log2 ratio) -2 -1 0 1at 175.555 kb on + strand, within Psest_0181at 175.556 kb on - strand, within Psest_0181at 175.556 kb on - strand, within Psest_0181at 175.556 kb on - strand, within Psest_0181at 175.556 kb on - strand, within Psest_0181at 175.615 kb on + strand, within Psest_0181at 175.615 kb on + strand, within Psest_0181at 175.615 kb on + strand, within Psest_0181at 175.667 kb on - strand, within Psest_0181at 175.667 kb on - strand, within Psest_0181at 175.924 kb on + strand, within Psest_0181at 176.307 kb on + strand, within Psest_0181at 176.308 kb on - strand, within Psest_0181at 176.308 kb on - strand, within Psest_0181at 176.308 kb on - strand, within Psest_0181at 176.308 kb on - strand, within Psest_0181at 176.308 kb on - strand, within Psest_0181at 176.435 kb on + strandat 176.435 kb on + strandat 176.436 kb on - strandat 176.436 kb on - strandat 176.443 kb on + strandat 176.443 kb on + strandat 176.444 kb on - strandat 176.444 kb on - strandat 176.622 kb on - strand, within Psest_0182at 176.622 kb on - strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.708 kb on + strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.709 kb on - strand, within Psest_0182at 176.786 kb on + strand, within Psest_0182at 176.940 kb on - strandat 176.940 kb on - strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.953 kb on + strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.954 kb on - strandat 176.955 kb on + strandat 176.955 kb on + strandat 176.955 kb on + strandat 176.955 kb on + strandat 176.955 kb on + strandat 177.278 kb on - strand, within Psest_0183at 177.278 kb on - strand, within Psest_0183at 177.286 kb on + strand, within Psest_0183at 177.286 kb on + strand, within Psest_0183at 177.286 kb on + strand, within Psest_0183at 177.286 kb on + strand, within Psest_0183at 177.287 kb on - strand, within Psest_0183at 177.287 kb on - strand, within Psest_0183at 177.341 kb on - strand, within Psest_0183at 177.341 kb on - strand, within Psest_0183at 177.341 kb on - strand, within Psest_0183at 177.341 kb on - strand, within Psest_0183at 177.803 kb on - strand, within Psest_0183

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Per-strain Table

Position Strand Gene LocusTag Fraction Def 5-Hydroxymethylfurfural 5 mM
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175,555 + Psest_0181 0.18 +0.2
175,556 - Psest_0181 0.18 -0.8
175,556 - Psest_0181 0.18 -0.3
175,556 - Psest_0181 0.18 -1.0
175,556 - Psest_0181 0.18 +0.5
175,615 + Psest_0181 0.23 +0.2
175,615 + Psest_0181 0.23 +0.2
175,615 + Psest_0181 0.23 -0.5
175,667 - Psest_0181 0.28 -1.8
175,667 - Psest_0181 0.28 +0.7
175,924 + Psest_0181 0.52 +0.9
176,307 + Psest_0181 0.87 +1.0
176,308 - Psest_0181 0.87 -0.5
176,308 - Psest_0181 0.87 -0.5
176,308 - Psest_0181 0.87 -0.1
176,308 - Psest_0181 0.87 +0.2
176,308 - Psest_0181 0.87 -0.3
176,435 + +0.8
176,435 + -1.4
176,436 - +0.0
176,436 - -0.1
176,443 + +0.3
176,443 + -0.0
176,444 - -0.9
176,444 - -2.1
176,622 - Psest_0182 0.32 +0.1
176,622 - Psest_0182 0.32 -0.9
176,708 + Psest_0182 0.54 -1.4
176,708 + Psest_0182 0.54 +0.2
176,708 + Psest_0182 0.54 -0.4
176,708 + Psest_0182 0.54 -0.1
176,708 + Psest_0182 0.54 +0.2
176,708 + Psest_0182 0.54 -1.6
176,709 - Psest_0182 0.54 -0.9
176,709 - Psest_0182 0.54 -0.9
176,709 - Psest_0182 0.54 -1.2
176,709 - Psest_0182 0.54 -2.6
176,709 - Psest_0182 0.54 -1.0
176,709 - Psest_0182 0.54 -1.1
176,709 - Psest_0182 0.54 -0.5
176,709 - Psest_0182 0.54 -1.6
176,709 - Psest_0182 0.54 -1.1
176,786 + Psest_0182 0.74 -1.6
176,940 - +0.2
176,940 - +0.7
176,953 + +0.3
176,953 + -0.9
176,953 + +0.5
176,953 + +0.7
176,953 + -0.1
176,953 + -0.4
176,953 + -1.8
176,953 + -0.2
176,954 - +0.5
176,954 - +0.4
176,954 - -1.4
176,954 - +0.2
176,954 - +0.3
176,954 - +0.7
176,954 - +0.3
176,954 - +1.1
176,955 + +0.8
176,955 + -0.3
176,955 + +0.2
176,955 + +0.5
176,955 + -2.5
177,278 - Psest_0183 0.12 -1.4
177,278 - Psest_0183 0.12 +1.2
177,286 + Psest_0183 0.12 +1.7
177,286 + Psest_0183 0.12 +0.0
177,286 + Psest_0183 0.12 -0.7
177,286 + Psest_0183 0.12 +0.9
177,287 - Psest_0183 0.12 +0.6
177,287 - Psest_0183 0.12 +1.2
177,341 - Psest_0183 0.14 -2.2
177,341 - Psest_0183 0.14 +0.8
177,341 - Psest_0183 0.14 +0.2
177,341 - Psest_0183 0.14 +0.3
177,803 - Psest_0183 0.30 -0.1

Or see this region's nucleotide sequence