Experiment: Def 5-Hydroxymethylfurfural 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1423 and Psest_1424 are separated by 381 nucleotides Psest_1424 and Psest_1425 overlap by 4 nucleotides Psest_1425 and Psest_1426 are separated by 2 nucleotides Psest_1426 and Psest_1427 are separated by 5 nucleotides
Psest_1423: Psest_1423 - PQQ-dependent dehydrogenase, methanol/ethanol family, at 1,531,596 to 1,533,728
_1423
Psest_1424: Psest_1424 - Lipoate-protein ligase A, at 1,534,110 to 1,534,877
_1424
Psest_1425: Psest_1425 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,534,874 to 1,535,575
_1425
Psest_1426: Psest_1426 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,535,578 to 1,535,811
_1426
Psest_1427: Psest_1427 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 1,535,817 to 1,536,839
_1427
Position (kb)
1534
1535 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1533.134 kb on + strand, within Psest_1423 at 1533.138 kb on + strand, within Psest_1423 at 1533.138 kb on + strand, within Psest_1423 at 1533.139 kb on - strand, within Psest_1423 at 1533.144 kb on + strand, within Psest_1423 at 1533.144 kb on + strand, within Psest_1423 at 1533.144 kb on + strand at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.145 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.280 kb on - strand, within Psest_1423 at 1533.312 kb on - strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.436 kb on + strand, within Psest_1423 at 1533.437 kb on - strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.439 kb on + strand, within Psest_1423 at 1533.440 kb on - strand, within Psest_1423 at 1533.440 kb on - strand, within Psest_1423 at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.534 kb on + strand at 1533.535 kb on - strand at 1533.602 kb on + strand at 1533.636 kb on - strand at 1533.732 kb on + strand at 1533.817 kb on + strand at 1533.817 kb on + strand at 1533.875 kb on + strand at 1533.875 kb on + strand at 1533.876 kb on - strand at 1533.876 kb on - strand at 1533.876 kb on - strand at 1533.900 kb on - strand at 1533.903 kb on + strand at 1533.912 kb on - strand at 1533.913 kb on + strand at 1534.000 kb on + strand at 1534.000 kb on + strand at 1534.000 kb on + strand at 1534.001 kb on - strand at 1534.001 kb on - strand at 1534.001 kb on - strand at 1534.010 kb on + strand at 1534.107 kb on + strand at 1534.210 kb on + strand, within Psest_1424 at 1534.211 kb on - strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.315 kb on + strand, within Psest_1424 at 1534.620 kb on - strand, within Psest_1424 at 1534.884 kb on + strand at 1534.894 kb on + strand at 1535.579 kb on + strand at 1535.811 kb on + strand at 1535.811 kb on + strand at 1535.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def 5-Hydroxymethylfurfural 5 mM remove 1,533,134 + Psest_1423 0.72 +1.6 1,533,138 + Psest_1423 0.72 -1.5 1,533,138 + Psest_1423 0.72 +1.7 1,533,139 - Psest_1423 0.72 -0.2 1,533,144 + Psest_1423 0.73 -0.4 1,533,144 + Psest_1423 0.73 -0.5 1,533,144 + +1.1 1,533,145 - Psest_1423 0.73 -0.5 1,533,145 - Psest_1423 0.73 -1.1 1,533,145 - Psest_1423 0.73 +0.5 1,533,145 - Psest_1423 0.73 -0.8 1,533,280 - Psest_1423 0.79 +0.1 1,533,280 - Psest_1423 0.79 +0.1 1,533,280 - Psest_1423 0.79 +0.1 1,533,280 - Psest_1423 0.79 -0.9 1,533,312 - Psest_1423 0.80 +0.8 1,533,436 + Psest_1423 0.86 +1.1 1,533,436 + Psest_1423 0.86 -0.1 1,533,436 + Psest_1423 0.86 +2.1 1,533,436 + Psest_1423 0.86 +0.1 1,533,436 + Psest_1423 0.86 -0.8 1,533,436 + Psest_1423 0.86 +0.5 1,533,436 + Psest_1423 0.86 +0.1 1,533,437 - Psest_1423 0.86 -0.5 1,533,439 + Psest_1423 0.86 +0.1 1,533,439 + Psest_1423 0.86 -0.9 1,533,439 + Psest_1423 0.86 +1.1 1,533,439 + Psest_1423 0.86 +2.1 1,533,440 - Psest_1423 0.86 -0.6 1,533,440 - Psest_1423 0.86 +1.1 1,533,534 + +0.8 1,533,534 + +0.1 1,533,534 + -0.4 1,533,534 + -0.5 1,533,535 - +3.1 1,533,602 + +0.1 1,533,636 - +0.4 1,533,732 + -0.1 1,533,817 + -0.9 1,533,817 + +1.1 1,533,875 + -0.9 1,533,875 + +1.1 1,533,876 - -0.9 1,533,876 - +0.5 1,533,876 - -0.5 1,533,900 - -0.3 1,533,903 + -0.2 1,533,912 - -0.9 1,533,913 + +1.1 1,534,000 + +2.1 1,534,000 + -1.3 1,534,000 + -1.2 1,534,001 - -1.5 1,534,001 - +0.6 1,534,001 - -0.9 1,534,010 + -0.5 1,534,107 + -0.9 1,534,210 + Psest_1424 0.13 -1.2 1,534,211 - Psest_1424 0.13 -0.5 1,534,315 + Psest_1424 0.27 +0.1 1,534,315 + Psest_1424 0.27 -0.9 1,534,315 + Psest_1424 0.27 +1.7 1,534,315 + Psest_1424 0.27 +1.1 1,534,315 + Psest_1424 0.27 -0.7 1,534,620 - Psest_1424 0.66 -0.9 1,534,884 + +0.4 1,534,894 + +0.5 1,535,579 + +0.1 1,535,811 + -0.2 1,535,811 + -0.5 1,535,812 - +0.4
Or see this region's nucleotide sequence