Experiment: Def 2-Furfuraldehyde 0.032 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2297 and Psest_2298 are separated by 150 nucleotides Psest_2298 and Psest_2299 are separated by 51 nucleotides Psest_2299 and Psest_2300 are separated by 32 nucleotides Psest_2300 and Psest_2301 are separated by 273 nucleotides Psest_2301 and Psest_2302 are separated by 58 nucleotides
Psest_2297: Psest_2297 - hypothetical protein, at 2,448,974 to 2,450,401
_2297
Psest_2298: Psest_2298 - tRNA_Leu_TAG, at 2,450,552 to 2,450,636
_2298
Psest_2299: Psest_2299 - tRNA_His_GTG, at 2,450,688 to 2,450,763
_2299
Psest_2300: Psest_2300 - tRNA_Arg_TCT, at 2,450,796 to 2,450,872
_2300
Psest_2301: Psest_2301 - 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase, at 2,451,146 to 2,452,000
_2301
Psest_2302: Psest_2302 - cysteinyl-tRNA synthetase, at 2,452,059 to 2,453,447
_2302
Position (kb)
2451
2452
2453 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2450.184 kb on + strand, within Psest_2297 at 2450.185 kb on - strand, within Psest_2297 at 2450.452 kb on + strand at 2450.453 kb on - strand at 2450.456 kb on + strand at 2450.456 kb on + strand at 2450.457 kb on - strand at 2450.457 kb on - strand at 2450.529 kb on + strand at 2450.530 kb on - strand at 2450.530 kb on - strand at 2450.876 kb on - strand at 2451.037 kb on + strand at 2452.048 kb on + strand at 2452.048 kb on + strand at 2452.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def 2-Furfuraldehyde 0.032 vol% remove 2,450,184 + Psest_2297 0.85 -1.0 2,450,185 - Psest_2297 0.85 -2.0 2,450,452 + +0.7 2,450,453 - +0.7 2,450,456 + -0.8 2,450,456 + -1.9 2,450,457 - -2.7 2,450,457 - +0.8 2,450,529 + -1.3 2,450,530 - +1.8 2,450,530 - +1.5 2,450,876 - -1.0 2,451,037 + -0.9 2,452,048 + -1.8 2,452,048 + -0.5 2,452,049 - -0.2
Or see this region's nucleotide sequence