Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1114

Experiment: Def 2-Furfuraldehyde 0.032 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1113 and Psest_1114 are separated by 42 nucleotidesPsest_1114 and Psest_1115 are separated by 21 nucleotidesPsest_1115 and Psest_1116 overlap by 8 nucleotides Psest_1113: Psest_1113 - peptide chain release factor 1, at 1,207,815 to 1,208,897 _1113 Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767 _1114 Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538 _1115 Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325 _1116 Position (kb) 1208 1209 1210Strain fitness (log2 ratio) -3 -2 -1 0 1at 1208.902 kb on + strandat 1208.902 kb on + strandat 1209.927 kb on + strand, within Psest_1115at 1209.927 kb on + strand, within Psest_1115at 1209.974 kb on + strand, within Psest_1115at 1210.340 kb on + strand, within Psest_1115

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Per-strain Table

Position Strand Gene LocusTag Fraction Def 2-Furfuraldehyde 0.032 vol%
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1,208,902 + +1.7
1,208,902 + -1.6
1,209,927 + Psest_1115 0.18 -3.7
1,209,927 + Psest_1115 0.18 -3.1
1,209,974 + Psest_1115 0.25 -2.0
1,210,340 + Psest_1115 0.73 -1.1

Or see this region's nucleotide sequence