Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3283 and Psest_3284 are separated by 33 nucleotides Psest_3284 and Psest_3285 overlap by 4 nucleotides Psest_3285 and Psest_3286 are separated by 142 nucleotides Psest_3286 and Psest_3287 are separated by 2 nucleotides
Psest_3283: Psest_3283 - Predicted membrane protein, at 3,450,070 to 3,450,747
_3283
Psest_3284: Psest_3284 - Uncharacterized protein conserved in bacteria, at 3,450,781 to 3,450,975
_3284
Psest_3285: Psest_3285 - dephospho-CoA kinase, at 3,450,972 to 3,451,580
_3285
Psest_3286: Psest_3286 - Type II secretory pathway, prepilin signal peptidase PulO and related peptidases, at 3,451,723 to 3,452,592
_3286
Psest_3287: Psest_3287 - Type II secretory pathway, component PulF, at 3,452,595 to 3,453,812
_3287
Position (kb)
3451
3452
3453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3450.935 kb on - strand, within Psest_3284 at 3451.620 kb on + strand at 3451.620 kb on + strand at 3451.620 kb on + strand at 3451.621 kb on - strand at 3451.623 kb on + strand at 3451.624 kb on - strand at 3451.657 kb on + strand at 3451.665 kb on - strand at 3451.685 kb on + strand at 3451.686 kb on - strand at 3451.745 kb on + strand at 3451.745 kb on + strand at 3451.746 kb on - strand at 3451.746 kb on - strand at 3451.861 kb on - strand, within Psest_3286 at 3452.003 kb on + strand, within Psest_3286 at 3452.004 kb on - strand, within Psest_3286 at 3452.025 kb on - strand, within Psest_3286 at 3452.079 kb on + strand, within Psest_3286 at 3452.232 kb on - strand, within Psest_3286 at 3452.232 kb on - strand, within Psest_3286 at 3452.340 kb on + strand, within Psest_3286 at 3452.341 kb on - strand, within Psest_3286 at 3452.442 kb on - strand, within Psest_3286 at 3452.457 kb on + strand, within Psest_3286 at 3452.458 kb on - strand, within Psest_3286 at 3452.479 kb on - strand, within Psest_3286 at 3452.545 kb on + strand at 3452.545 kb on + strand at 3452.546 kb on - strand at 3452.577 kb on - strand at 3452.596 kb on + strand at 3452.597 kb on - strand at 3452.629 kb on + strand at 3452.629 kb on + strand at 3452.629 kb on + strand at 3452.630 kb on - strand at 3452.630 kb on - strand at 3452.630 kb on - strand at 3452.630 kb on - strand at 3452.633 kb on + strand at 3452.634 kb on - strand at 3452.634 kb on - strand at 3452.714 kb on + strand at 3452.714 kb on + strand at 3452.714 kb on + strand at 3452.734 kb on + strand, within Psest_3287 at 3452.734 kb on + strand, within Psest_3287 at 3452.734 kb on + strand, within Psest_3287 at 3452.831 kb on + strand, within Psest_3287 at 3452.831 kb on + strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.832 kb on - strand, within Psest_3287 at 3452.856 kb on - strand, within Psest_3287 at 3452.856 kb on - strand, within Psest_3287 at 3452.969 kb on + strand, within Psest_3287 at 3452.970 kb on - strand, within Psest_3287 at 3453.011 kb on - strand, within Psest_3287 at 3453.405 kb on + strand, within Psest_3287 at 3453.405 kb on + strand, within Psest_3287 at 3453.464 kb on + strand, within Psest_3287
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 3,450,935 - Psest_3284 0.79 +0.7 3,451,620 + -1.3 3,451,620 + +0.3 3,451,620 + -1.0 3,451,621 - +0.0 3,451,623 + +0.8 3,451,624 - +1.2 3,451,657 + -0.6 3,451,665 - -0.8 3,451,685 + -0.5 3,451,686 - -0.9 3,451,745 + -2.2 3,451,745 + +0.7 3,451,746 - -0.9 3,451,746 - +2.6 3,451,861 - Psest_3286 0.16 -1.9 3,452,003 + Psest_3286 0.32 -1.7 3,452,004 - Psest_3286 0.32 -2.3 3,452,025 - Psest_3286 0.35 -1.3 3,452,079 + Psest_3286 0.41 +0.3 3,452,232 - Psest_3286 0.59 -2.3 3,452,232 - Psest_3286 0.59 -1.8 3,452,340 + Psest_3286 0.71 -0.6 3,452,341 - Psest_3286 0.71 -1.8 3,452,442 - Psest_3286 0.83 -2.0 3,452,457 + Psest_3286 0.84 -1.2 3,452,458 - Psest_3286 0.84 -2.2 3,452,479 - Psest_3286 0.87 -1.6 3,452,545 + -2.7 3,452,545 + -0.6 3,452,546 - -1.1 3,452,577 - -0.5 3,452,596 + -2.2 3,452,597 - +0.7 3,452,629 + +1.0 3,452,629 + -1.6 3,452,629 + -3.2 3,452,630 - +1.0 3,452,630 - -0.6 3,452,630 - +1.0 3,452,630 - -2.9 3,452,633 + -2.4 3,452,634 - -1.2 3,452,634 - -1.7 3,452,714 + -1.0 3,452,714 + -1.9 3,452,714 + -1.2 3,452,734 + Psest_3287 0.11 -1.3 3,452,734 + Psest_3287 0.11 -2.7 3,452,734 + Psest_3287 0.11 -1.1 3,452,831 + Psest_3287 0.19 -1.2 3,452,831 + Psest_3287 0.19 +0.2 3,452,832 - Psest_3287 0.19 -1.6 3,452,832 - Psest_3287 0.19 -1.3 3,452,832 - Psest_3287 0.19 -1.0 3,452,832 - Psest_3287 0.19 -0.6 3,452,832 - Psest_3287 0.19 -1.8 3,452,832 - Psest_3287 0.19 -1.9 3,452,856 - Psest_3287 0.21 -0.2 3,452,856 - Psest_3287 0.21 -0.7 3,452,969 + Psest_3287 0.31 -2.2 3,452,970 - Psest_3287 0.31 -0.4 3,453,011 - Psest_3287 0.34 -0.7 3,453,405 + Psest_3287 0.67 -0.1 3,453,405 + Psest_3287 0.67 +0.0 3,453,464 + Psest_3287 0.71 -1.6
Or see this region's nucleotide sequence