Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0285

Experiment: Def Benzalkonium Chloride 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0283 and Psest_0284 are separated by 10 nucleotidesPsest_0284 and Psest_0285 are separated by 66 nucleotidesPsest_0285 and Psest_0286 are separated by 59 nucleotides Psest_0283: Psest_0283 - pyrroline-5-carboxylate reductase, at 298,749 to 299,573 _0283 Psest_0284: Psest_0284 - pyridoxal phosphate enzyme, YggS family, at 299,584 to 300,273 _0284 Psest_0285: Psest_0285 - pilus retraction protein PilT, at 300,340 to 301,374 _0285 Psest_0286: Psest_0286 - pilus retraction protein PilT, at 301,434 to 302,579 _0286 Position (kb) 300 301 302Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 299.573 kb on + strandat 299.574 kb on - strandat 299.574 kb on - strandat 299.574 kb on - strandat 299.574 kb on - strandat 299.574 kb on - strandat 299.698 kb on - strand, within Psest_0284at 299.845 kb on + strand, within Psest_0284at 299.845 kb on + strand, within Psest_0284at 299.846 kb on - strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.908 kb on + strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.909 kb on - strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.910 kb on + strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.911 kb on - strand, within Psest_0284at 299.923 kb on + strand, within Psest_0284at 299.923 kb on + strand, within Psest_0284at 299.924 kb on - strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.101 kb on + strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.102 kb on - strand, within Psest_0284at 300.252 kb on + strandat 300.252 kb on + strandat 300.253 kb on - strandat 300.253 kb on - strandat 300.253 kb on - strandat 300.253 kb on - strandat 300.274 kb on + strandat 300.274 kb on + strandat 300.274 kb on + strandat 300.274 kb on + strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.275 kb on - strandat 300.311 kb on + strandat 300.311 kb on + strandat 300.311 kb on + strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.312 kb on - strandat 300.326 kb on - strandat 300.326 kb on - strandat 300.346 kb on - strandat 300.493 kb on + strand, within Psest_0285at 300.493 kb on + strand, within Psest_0285at 300.493 kb on + strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.776 kb on - strand, within Psest_0285at 300.793 kb on + strand, within Psest_0285at 300.793 kb on + strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.323 kb on + strandat 301.323 kb on + strandat 301.323 kb on + strandat 301.323 kb on + strandat 301.324 kb on - strandat 301.372 kb on + strandat 301.373 kb on - strandat 301.427 kb on + strandat 301.428 kb on - strandat 301.693 kb on - strand, within Psest_0286at 301.693 kb on - strand, within Psest_0286at 302.070 kb on + strand, within Psest_0286at 302.070 kb on + strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml
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299,573 + -0.6
299,574 - -2.3
299,574 - -1.5
299,574 - +2.3
299,574 - -0.6
299,574 - +0.2
299,698 - Psest_0284 0.17 +0.4
299,845 + Psest_0284 0.38 +1.9
299,845 + Psest_0284 0.38 +0.8
299,846 - Psest_0284 0.38 +1.6
299,908 + Psest_0284 0.47 +1.3
299,908 + Psest_0284 0.47 +1.5
299,908 + Psest_0284 0.47 +0.6
299,908 + Psest_0284 0.47 +0.4
299,908 + Psest_0284 0.47 +0.9
299,908 + Psest_0284 0.47 +1.2
299,908 + Psest_0284 0.47 -0.0
299,909 - Psest_0284 0.47 +1.6
299,909 - Psest_0284 0.47 +0.1
299,909 - Psest_0284 0.47 +1.1
299,909 - Psest_0284 0.47 -0.3
299,909 - Psest_0284 0.47 +1.9
299,909 - Psest_0284 0.47 +0.4
299,909 - Psest_0284 0.47 +1.1
299,909 - Psest_0284 0.47 +0.2
299,909 - Psest_0284 0.47 -0.6
299,909 - Psest_0284 0.47 +1.1
299,909 - Psest_0284 0.47 -0.6
299,909 - Psest_0284 0.47 +0.9
299,909 - Psest_0284 0.47 +1.3
299,909 - Psest_0284 0.47 +0.7
299,909 - Psest_0284 0.47 -0.1
299,909 - Psest_0284 0.47 +0.5
299,909 - Psest_0284 0.47 +0.6
299,909 - Psest_0284 0.47 +1.7
299,909 - Psest_0284 0.47 +0.3
299,909 - Psest_0284 0.47 +0.6
299,909 - Psest_0284 0.47 +1.8
299,910 + Psest_0284 0.47 -0.6
299,910 + Psest_0284 0.47 +0.1
299,910 + Psest_0284 0.47 -1.0
299,910 + Psest_0284 0.47 -0.6
299,910 + Psest_0284 0.47 -0.3
299,910 + Psest_0284 0.47 -1.0
299,910 + Psest_0284 0.47 +1.5
299,910 + Psest_0284 0.47 +1.0
299,911 - Psest_0284 0.47 +1.3
299,911 - Psest_0284 0.47 -0.7
299,911 - Psest_0284 0.47 -1.0
299,911 - Psest_0284 0.47 +0.8
299,911 - Psest_0284 0.47 +1.3
299,911 - Psest_0284 0.47 -0.1
299,911 - Psest_0284 0.47 +1.6
299,911 - Psest_0284 0.47 +0.4
299,911 - Psest_0284 0.47 +0.9
299,911 - Psest_0284 0.47 +0.5
299,911 - Psest_0284 0.47 +1.7
299,911 - Psest_0284 0.47 +1.7
299,911 - Psest_0284 0.47 +0.7
299,911 - Psest_0284 0.47 +1.3
299,911 - Psest_0284 0.47 -0.9
299,911 - Psest_0284 0.47 -0.2
299,911 - Psest_0284 0.47 -0.8
299,911 - Psest_0284 0.47 +1.3
299,911 - Psest_0284 0.47 +0.0
299,911 - Psest_0284 0.47 +1.3
299,923 + Psest_0284 0.49 +1.4
299,923 + Psest_0284 0.49 -1.8
299,924 - Psest_0284 0.49 +0.6
300,101 + Psest_0284 0.75 -0.6
300,101 + Psest_0284 0.75 -2.8
300,101 + Psest_0284 0.75 -1.6
300,101 + Psest_0284 0.75 -0.6
300,101 + Psest_0284 0.75 -1.3
300,101 + Psest_0284 0.75 -1.7
300,102 - Psest_0284 0.75 +0.0
300,102 - Psest_0284 0.75 -0.7
300,102 - Psest_0284 0.75 +0.0
300,102 - Psest_0284 0.75 -0.3
300,102 - Psest_0284 0.75 +0.7
300,102 - Psest_0284 0.75 -0.3
300,252 + -0.6
300,252 + -3.6
300,253 - +0.9
300,253 - +0.4
300,253 - +0.5
300,253 - +2.3
300,274 + -0.0
300,274 + -0.3
300,274 + -0.9
300,274 + -2.3
300,275 - -1.1
300,275 - -2.8
300,275 - -2.7
300,275 - -2.0
300,275 - -0.4
300,275 - -0.6
300,275 - -2.4
300,275 - -3.1
300,275 - -3.1
300,275 - -2.6
300,275 - -1.5
300,275 - -3.3
300,275 - -1.3
300,311 + -0.9
300,311 + -0.1
300,311 + -1.1
300,312 - -1.3
300,312 - -2.8
300,312 - -2.9
300,312 - -3.0
300,312 - -2.8
300,312 - -4.0
300,312 - -4.1
300,312 - -4.6
300,312 - -2.0
300,312 - -0.0
300,312 - -2.5
300,312 - -1.0
300,326 - +0.3
300,326 - -3.5
300,346 - -2.2
300,493 + Psest_0285 0.15 -1.3
300,493 + Psest_0285 0.15 -0.6
300,493 + Psest_0285 0.15 -2.2
300,494 - Psest_0285 0.15 -3.1
300,494 - Psest_0285 0.15 -3.1
300,494 - Psest_0285 0.15 -2.5
300,494 - Psest_0285 0.15 -3.5
300,494 - Psest_0285 0.15 -2.7
300,494 - Psest_0285 0.15 -0.1
300,776 - Psest_0285 0.42 -1.6
300,793 + Psest_0285 0.44 -4.9
300,793 + Psest_0285 0.44 -5.0
300,794 - Psest_0285 0.44 -2.6
300,794 - Psest_0285 0.44 -3.1
300,794 - Psest_0285 0.44 -1.3
301,243 + Psest_0285 0.87 -3.4
301,243 + Psest_0285 0.87 -4.8
301,243 + Psest_0285 0.87 -5.2
301,243 + Psest_0285 0.87 -2.7
301,243 + Psest_0285 0.87 -2.0
301,243 + Psest_0285 0.87 -2.0
301,243 + Psest_0285 0.87 -1.3
301,243 + Psest_0285 0.87 -2.4
301,243 + Psest_0285 0.87 -2.6
301,243 + Psest_0285 0.87 -2.2
301,244 - Psest_0285 0.87 -4.2
301,244 - Psest_0285 0.87 -3.2
301,244 - Psest_0285 0.87 -3.0
301,244 - Psest_0285 0.87 -2.9
301,244 - Psest_0285 0.87 -2.1
301,244 - Psest_0285 0.87 -3.4
301,244 - Psest_0285 0.87 -3.8
301,244 - Psest_0285 0.87 -4.0
301,244 - Psest_0285 0.87 -5.3
301,244 - Psest_0285 0.87 -2.8
301,244 - Psest_0285 0.87 -2.6
301,323 + -2.6
301,323 + -2.5
301,323 + -2.7
301,323 + -2.5
301,324 - -3.2
301,372 + -1.3
301,373 - -0.7
301,427 + -1.0
301,428 - -0.6
301,693 - Psest_0286 0.23 -0.8
301,693 - Psest_0286 0.23 -0.5
302,070 + Psest_0286 0.55 -0.5
302,070 + Psest_0286 0.55 -1.4
302,071 - Psest_0286 0.56 -2.3
302,071 - Psest_0286 0.56 -1.3
302,071 - Psest_0286 0.56 -1.1

Or see this region's nucleotide sequence