Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2320 and Psest_2321 are separated by 92 nucleotides Psest_2321 and Psest_2322 overlap by 4 nucleotides Psest_2322 and Psest_2323 are separated by 171 nucleotides
Psest_2320: Psest_2320 - 2-methylcitrate synthase/citrate synthase II, at 2,474,031 to 2,475,158
_2320
Psest_2321: Psest_2321 - methylisocitrate lyase, at 2,475,251 to 2,476,144
_2321
Psest_2322: Psest_2322 - Transcriptional regulators, at 2,476,141 to 2,476,866
_2322
Psest_2323: Psest_2323 - Uncharacterized protein conserved in archaea, at 2,477,038 to 2,477,670
_2323
Position (kb)
2475
2476
2477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2474.288 kb on - strand, within Psest_2320 at 2474.368 kb on + strand, within Psest_2320 at 2474.368 kb on + strand, within Psest_2320 at 2474.539 kb on + strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.891 kb on + strand, within Psest_2320 at 2474.953 kb on + strand, within Psest_2320 at 2474.953 kb on + strand, within Psest_2320 at 2474.989 kb on + strand, within Psest_2320 at 2474.990 kb on - strand, within Psest_2320 at 2475.163 kb on - strand at 2475.253 kb on - strand at 2475.253 kb on - strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.309 kb on + strand at 2475.310 kb on - strand at 2475.310 kb on - strand at 2475.394 kb on - strand, within Psest_2321 at 2475.486 kb on + strand, within Psest_2321 at 2475.487 kb on - strand, within Psest_2321 at 2475.487 kb on - strand, within Psest_2321 at 2475.558 kb on + strand, within Psest_2321 at 2475.558 kb on + strand, within Psest_2321 at 2475.925 kb on + strand, within Psest_2321 at 2475.932 kb on + strand, within Psest_2321 at 2475.932 kb on + strand, within Psest_2321 at 2475.932 kb on + strand, within Psest_2321 at 2475.932 kb on + strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2475.933 kb on - strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.017 kb on + strand, within Psest_2321 at 2476.018 kb on - strand, within Psest_2321 at 2476.018 kb on - strand, within Psest_2321 at 2476.018 kb on - strand, within Psest_2321 at 2476.018 kb on - strand, within Psest_2321 at 2476.018 kb on - strand, within Psest_2321 at 2476.343 kb on + strand, within Psest_2322 at 2476.344 kb on - strand, within Psest_2322 at 2476.344 kb on - strand, within Psest_2322 at 2476.452 kb on - strand, within Psest_2322 at 2476.589 kb on + strand, within Psest_2322 at 2476.590 kb on - strand, within Psest_2322 at 2476.715 kb on + strand, within Psest_2322 at 2476.715 kb on + strand, within Psest_2322 at 2476.716 kb on - strand, within Psest_2322 at 2476.716 kb on - strand, within Psest_2322 at 2476.911 kb on + strand at 2476.911 kb on + strand at 2476.958 kb on - strand at 2476.981 kb on - strand at 2477.042 kb on + strand at 2477.042 kb on + strand at 2477.043 kb on - strand at 2477.043 kb on - strand at 2477.043 kb on - strand at 2477.043 kb on - strand at 2477.142 kb on + strand, within Psest_2323 at 2477.142 kb on + strand, within Psest_2323 at 2477.142 kb on + strand, within Psest_2323 at 2477.143 kb on - strand, within Psest_2323
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 2,474,288 - Psest_2320 0.23 +1.1 2,474,368 + Psest_2320 0.30 -0.3 2,474,368 + Psest_2320 0.30 +0.2 2,474,539 + Psest_2320 0.45 +0.0 2,474,753 - Psest_2320 0.64 -0.5 2,474,753 - Psest_2320 0.64 +1.1 2,474,753 - Psest_2320 0.64 +0.3 2,474,891 + Psest_2320 0.76 +0.2 2,474,953 + Psest_2320 0.82 -1.8 2,474,953 + Psest_2320 0.82 -0.3 2,474,989 + Psest_2320 0.85 +1.1 2,474,990 - Psest_2320 0.85 -0.8 2,475,163 - -0.1 2,475,253 - +0.9 2,475,253 - +0.9 2,475,309 + -1.4 2,475,309 + +0.2 2,475,309 + -1.0 2,475,309 + +0.3 2,475,309 + +1.9 2,475,309 + +0.1 2,475,309 + -1.9 2,475,310 - +1.3 2,475,310 - +2.0 2,475,394 - Psest_2321 0.16 +1.9 2,475,486 + Psest_2321 0.26 +2.1 2,475,487 - Psest_2321 0.26 +1.6 2,475,487 - Psest_2321 0.26 +2.9 2,475,558 + Psest_2321 0.34 -1.3 2,475,558 + Psest_2321 0.34 +1.0 2,475,925 + Psest_2321 0.75 -0.1 2,475,932 + Psest_2321 0.76 -0.3 2,475,932 + Psest_2321 0.76 +1.7 2,475,932 + Psest_2321 0.76 +1.5 2,475,932 + Psest_2321 0.76 +1.5 2,475,933 - Psest_2321 0.76 -0.0 2,475,933 - Psest_2321 0.76 +0.9 2,475,933 - Psest_2321 0.76 +0.3 2,475,933 - Psest_2321 0.76 -0.3 2,475,933 - Psest_2321 0.76 +0.7 2,475,933 - Psest_2321 0.76 -0.3 2,476,017 + Psest_2321 0.86 +1.0 2,476,017 + Psest_2321 0.86 +1.6 2,476,017 + Psest_2321 0.86 +1.1 2,476,017 + Psest_2321 0.86 +0.5 2,476,017 + Psest_2321 0.86 +0.6 2,476,017 + Psest_2321 0.86 +2.5 2,476,018 - Psest_2321 0.86 +2.2 2,476,018 - Psest_2321 0.86 +0.7 2,476,018 - Psest_2321 0.86 -0.3 2,476,018 - Psest_2321 0.86 +0.8 2,476,018 - Psest_2321 0.86 +1.5 2,476,343 + Psest_2322 0.28 -0.8 2,476,344 - Psest_2322 0.28 +3.1 2,476,344 - Psest_2322 0.28 +1.8 2,476,452 - Psest_2322 0.43 -0.4 2,476,589 + Psest_2322 0.62 -1.7 2,476,590 - Psest_2322 0.62 +1.3 2,476,715 + Psest_2322 0.79 -1.3 2,476,715 + Psest_2322 0.79 -0.1 2,476,716 - Psest_2322 0.79 +2.1 2,476,716 - Psest_2322 0.79 -0.6 2,476,911 + +1.3 2,476,911 + -1.2 2,476,958 - -0.3 2,476,981 - -2.2 2,477,042 + -2.1 2,477,042 + +0.3 2,477,043 - -3.1 2,477,043 - +1.1 2,477,043 - -2.3 2,477,043 - -3.1 2,477,142 + Psest_2323 0.16 -1.3 2,477,142 + Psest_2323 0.16 -3.6 2,477,142 + Psest_2323 0.16 -2.8 2,477,143 - Psest_2323 0.17 -1.9
Or see this region's nucleotide sequence