Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0225 and Psest_0226 are separated by 29 nucleotides Psest_0226 and Psest_0227 overlap by 8 nucleotides
Psest_0225: Psest_0225 - Predicted permease, DMT superfamily, at 233,672 to 234,583
_0225
Psest_0226: Psest_0226 - Domain of unknown function (DUF3482)./GTPase of unknown function., at 234,613 to 235,986
_0226
Psest_0227: Psest_0227 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 235,979 to 237,373
_0227
Position (kb)
234
235
236 Strain fitness (log2 ratio)
-1
0
1
2
3 at 233.680 kb on + strand at 233.680 kb on + strand at 233.680 kb on + strand at 233.681 kb on - strand at 233.681 kb on - strand at 233.681 kb on - strand at 233.681 kb on - strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 233.750 kb on + strand at 234.108 kb on + strand, within Psest_0225 at 234.108 kb on + strand, within Psest_0225 at 234.109 kb on - strand, within Psest_0225 at 234.109 kb on - strand, within Psest_0225 at 234.321 kb on - strand, within Psest_0225 at 234.582 kb on - strand at 234.924 kb on - strand, within Psest_0226 at 235.073 kb on - strand, within Psest_0226 at 235.161 kb on - strand, within Psest_0226 at 235.161 kb on - strand, within Psest_0226 at 235.590 kb on - strand, within Psest_0226 at 235.590 kb on - strand, within Psest_0226 at 235.590 kb on - strand, within Psest_0226 at 235.590 kb on - strand, within Psest_0226 at 235.590 kb on - strand, within Psest_0226 at 235.625 kb on + strand, within Psest_0226 at 235.626 kb on - strand, within Psest_0226 at 235.626 kb on - strand, within Psest_0226 at 235.626 kb on - strand, within Psest_0226 at 235.626 kb on - strand, within Psest_0226 at 235.769 kb on + strand, within Psest_0226 at 236.105 kb on + strand at 236.562 kb on + strand, within Psest_0227 at 236.562 kb on + strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.563 kb on - strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.791 kb on + strand, within Psest_0227 at 236.792 kb on - strand, within Psest_0227 at 236.831 kb on + strand, within Psest_0227 at 236.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 233,680 + +0.1 233,680 + -0.3 233,680 + -1.1 233,681 - -0.1 233,681 - +0.7 233,681 - -0.6 233,681 - -0.1 233,750 + -0.5 233,750 + +0.9 233,750 + -0.1 233,750 + +0.4 233,750 + +0.4 233,750 + +0.9 233,750 + +0.9 233,750 + +0.1 233,750 + -0.6 233,750 + -0.4 234,108 + Psest_0225 0.48 +1.5 234,108 + Psest_0225 0.48 -0.1 234,109 - Psest_0225 0.48 +0.5 234,109 - Psest_0225 0.48 -0.1 234,321 - Psest_0225 0.71 +0.7 234,582 - +0.5 234,924 - Psest_0226 0.23 +0.9 235,073 - Psest_0226 0.33 +1.9 235,161 - Psest_0226 0.40 +1.3 235,161 - Psest_0226 0.40 +2.4 235,590 - Psest_0226 0.71 +1.9 235,590 - Psest_0226 0.71 +1.7 235,590 - Psest_0226 0.71 +2.9 235,590 - Psest_0226 0.71 -0.3 235,590 - Psest_0226 0.71 +0.9 235,625 + Psest_0226 0.74 +1.4 235,626 - Psest_0226 0.74 +0.2 235,626 - Psest_0226 0.74 +1.9 235,626 - Psest_0226 0.74 +2.9 235,626 - Psest_0226 0.74 -0.1 235,769 + Psest_0226 0.84 +0.9 236,105 + +0.7 236,562 + Psest_0227 0.42 +0.9 236,562 + Psest_0227 0.42 -0.1 236,563 - Psest_0227 0.42 -0.1 236,563 - Psest_0227 0.42 +0.6 236,563 - Psest_0227 0.42 +0.9 236,791 + Psest_0227 0.58 -0.1 236,791 + Psest_0227 0.58 -0.6 236,791 + Psest_0227 0.58 +0.9 236,792 - Psest_0227 0.58 +0.9 236,831 + Psest_0227 0.61 +0.9 236,843 + +0.9
Or see this region's nucleotide sequence