Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2073 and Psest_2074 are separated by 14 nucleotides Psest_2074 and Psest_2075 are separated by 83 nucleotides Psest_2075 and Psest_2076 are separated by 182 nucleotides
Psest_2073: Psest_2073 - Ribonuclease HI, at 2,202,015 to 2,202,470
_2073
Psest_2074: Psest_2074 - DNA polymerase III, epsilon subunit, Proteobacterial, at 2,202,485 to 2,203,210
_2074
Psest_2075: Psest_2075 - Arginine/lysine/ornithine decarboxylases, at 2,203,294 to 2,205,543
_2075
Psest_2076: Psest_2076 - Enoyl-CoA hydratase/carnithine racemase, at 2,205,726 to 2,206,538
_2076
Position (kb)
2203
2204
2205
2206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2202.439 kb on + strand at 2202.448 kb on + strand at 2202.448 kb on + strand at 2202.468 kb on + strand at 2202.468 kb on + strand at 2203.261 kb on - strand at 2203.267 kb on + strand at 2203.268 kb on - strand at 2203.297 kb on + strand at 2203.298 kb on - strand at 2203.298 kb on - strand at 2203.298 kb on - strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.341 kb on - strand at 2203.341 kb on - strand at 2203.341 kb on - strand at 2203.370 kb on + strand at 2203.370 kb on + strand at 2203.371 kb on - strand at 2203.660 kb on + strand, within Psest_2075 at 2203.661 kb on - strand, within Psest_2075 at 2203.661 kb on - strand, within Psest_2075 at 2203.666 kb on + strand, within Psest_2075 at 2203.667 kb on - strand, within Psest_2075 at 2203.667 kb on - strand, within Psest_2075 at 2203.667 kb on - strand, within Psest_2075 at 2203.720 kb on + strand, within Psest_2075 at 2203.721 kb on - strand, within Psest_2075 at 2203.783 kb on + strand, within Psest_2075 at 2203.784 kb on - strand, within Psest_2075 at 2203.784 kb on - strand, within Psest_2075 at 2203.784 kb on - strand, within Psest_2075 at 2204.131 kb on + strand, within Psest_2075 at 2204.131 kb on + strand, within Psest_2075 at 2204.131 kb on + strand, within Psest_2075 at 2204.131 kb on + strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.132 kb on - strand, within Psest_2075 at 2204.156 kb on + strand, within Psest_2075 at 2204.156 kb on + strand, within Psest_2075 at 2204.157 kb on - strand, within Psest_2075 at 2204.285 kb on - strand, within Psest_2075 at 2204.285 kb on - strand, within Psest_2075 at 2204.285 kb on - strand, within Psest_2075 at 2204.299 kb on + strand, within Psest_2075 at 2204.299 kb on + strand, within Psest_2075 at 2204.299 kb on + strand, within Psest_2075 at 2204.299 kb on + strand, within Psest_2075 at 2204.300 kb on - strand, within Psest_2075 at 2204.369 kb on - strand, within Psest_2075 at 2204.374 kb on + strand, within Psest_2075 at 2204.375 kb on - strand, within Psest_2075 at 2204.375 kb on - strand, within Psest_2075 at 2204.375 kb on - strand, within Psest_2075 at 2204.375 kb on - strand, within Psest_2075 at 2204.395 kb on + strand, within Psest_2075 at 2204.395 kb on + strand, within Psest_2075 at 2204.396 kb on - strand, within Psest_2075 at 2204.396 kb on - strand, within Psest_2075 at 2204.396 kb on - strand, within Psest_2075 at 2204.609 kb on + strand, within Psest_2075 at 2204.609 kb on + strand, within Psest_2075 at 2204.610 kb on - strand, within Psest_2075 at 2204.845 kb on + strand, within Psest_2075 at 2204.845 kb on + strand, within Psest_2075 at 2204.845 kb on + strand, within Psest_2075 at 2204.846 kb on - strand, within Psest_2075 at 2204.943 kb on + strand, within Psest_2075 at 2204.944 kb on - strand, within Psest_2075 at 2204.999 kb on + strand, within Psest_2075 at 2205.000 kb on - strand, within Psest_2075 at 2205.000 kb on - strand, within Psest_2075 at 2205.090 kb on - strand, within Psest_2075 at 2205.099 kb on - strand, within Psest_2075 at 2205.140 kb on - strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.226 kb on + strand, within Psest_2075 at 2205.227 kb on - strand, within Psest_2075 at 2205.227 kb on - strand, within Psest_2075 at 2205.511 kb on - strand at 2205.517 kb on + strand at 2205.517 kb on + strand at 2205.518 kb on - strand at 2205.608 kb on - strand at 2205.654 kb on - strand at 2205.655 kb on + strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.656 kb on - strand at 2205.735 kb on + strand at 2205.735 kb on + strand at 2205.736 kb on - strand at 2205.752 kb on - strand at 2205.843 kb on - strand, within Psest_2076 at 2206.123 kb on - strand, within Psest_2076 at 2206.244 kb on + strand, within Psest_2076 at 2206.245 kb on - strand, within Psest_2076 at 2206.302 kb on + strand, within Psest_2076 at 2206.302 kb on + strand, within Psest_2076 at 2206.303 kb on - strand, within Psest_2076 at 2206.303 kb on - strand, within Psest_2076 at 2206.303 kb on - strand, within Psest_2076 at 2206.303 kb on - strand, within Psest_2076 at 2206.405 kb on - strand, within Psest_2076 at 2206.406 kb on + strand, within Psest_2076 at 2206.406 kb on + strand, within Psest_2076 at 2206.407 kb on - strand, within Psest_2076 at 2206.440 kb on + strand, within Psest_2076 at 2206.440 kb on + strand, within Psest_2076 at 2206.441 kb on - strand, within Psest_2076 at 2206.505 kb on + strand at 2206.505 kb on + strand at 2206.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 2,202,439 + +2.2 2,202,448 + +0.9 2,202,448 + -0.9 2,202,468 + +0.5 2,202,468 + +0.2 2,203,261 - -0.2 2,203,267 + -2.2 2,203,268 - +1.0 2,203,297 + +1.4 2,203,298 - -0.7 2,203,298 - -0.3 2,203,298 - -0.5 2,203,340 + +0.5 2,203,340 + +1.7 2,203,340 + +0.9 2,203,340 + -0.7 2,203,341 - +1.4 2,203,341 - -2.0 2,203,341 - +0.3 2,203,370 + +1.1 2,203,370 + +1.3 2,203,371 - +1.3 2,203,660 + Psest_2075 0.16 +0.1 2,203,661 - Psest_2075 0.16 +2.0 2,203,661 - Psest_2075 0.16 -0.0 2,203,666 + Psest_2075 0.17 +0.1 2,203,667 - Psest_2075 0.17 -0.7 2,203,667 - Psest_2075 0.17 -0.5 2,203,667 - Psest_2075 0.17 +0.1 2,203,720 + Psest_2075 0.19 +0.2 2,203,721 - Psest_2075 0.19 -0.5 2,203,783 + Psest_2075 0.22 -0.6 2,203,784 - Psest_2075 0.22 +0.6 2,203,784 - Psest_2075 0.22 -1.4 2,203,784 - Psest_2075 0.22 -0.5 2,204,131 + Psest_2075 0.37 -0.9 2,204,131 + Psest_2075 0.37 +0.2 2,204,131 + Psest_2075 0.37 -1.2 2,204,131 + Psest_2075 0.37 -2.0 2,204,132 - Psest_2075 0.37 +0.6 2,204,132 - Psest_2075 0.37 +0.0 2,204,132 - Psest_2075 0.37 -0.0 2,204,132 - Psest_2075 0.37 +1.7 2,204,132 - Psest_2075 0.37 +0.0 2,204,132 - Psest_2075 0.37 +0.6 2,204,132 - Psest_2075 0.37 +0.5 2,204,156 + Psest_2075 0.38 -0.7 2,204,156 + Psest_2075 0.38 -0.3 2,204,157 - Psest_2075 0.38 -0.5 2,204,285 - Psest_2075 0.44 +0.4 2,204,285 - Psest_2075 0.44 +0.4 2,204,285 - Psest_2075 0.44 +0.7 2,204,299 + Psest_2075 0.45 -3.0 2,204,299 + Psest_2075 0.45 +1.3 2,204,299 + Psest_2075 0.45 +0.5 2,204,299 + Psest_2075 0.45 -0.4 2,204,300 - Psest_2075 0.45 +0.8 2,204,369 - Psest_2075 0.48 +0.6 2,204,374 + Psest_2075 0.48 +0.5 2,204,375 - Psest_2075 0.48 +1.6 2,204,375 - Psest_2075 0.48 +1.3 2,204,375 - Psest_2075 0.48 +1.0 2,204,375 - Psest_2075 0.48 +1.3 2,204,395 + Psest_2075 0.49 +0.3 2,204,395 + Psest_2075 0.49 -1.7 2,204,396 - Psest_2075 0.49 +0.6 2,204,396 - Psest_2075 0.49 +2.8 2,204,396 - Psest_2075 0.49 +0.0 2,204,609 + Psest_2075 0.58 +1.5 2,204,609 + Psest_2075 0.58 +0.5 2,204,610 - Psest_2075 0.58 +0.3 2,204,845 + Psest_2075 0.69 +0.3 2,204,845 + Psest_2075 0.69 +0.7 2,204,845 + Psest_2075 0.69 +1.3 2,204,846 - Psest_2075 0.69 -1.3 2,204,943 + Psest_2075 0.73 -0.1 2,204,944 - Psest_2075 0.73 -0.0 2,204,999 + Psest_2075 0.76 +0.6 2,205,000 - Psest_2075 0.76 +0.4 2,205,000 - Psest_2075 0.76 -0.2 2,205,090 - Psest_2075 0.80 -0.7 2,205,099 - Psest_2075 0.80 -1.7 2,205,140 - Psest_2075 0.82 +1.6 2,205,226 + Psest_2075 0.86 +0.2 2,205,226 + Psest_2075 0.86 -0.3 2,205,226 + Psest_2075 0.86 +0.4 2,205,226 + Psest_2075 0.86 +0.0 2,205,226 + Psest_2075 0.86 -0.7 2,205,226 + Psest_2075 0.86 +0.4 2,205,227 - Psest_2075 0.86 +1.2 2,205,227 - Psest_2075 0.86 -0.6 2,205,511 - -1.5 2,205,517 + +2.6 2,205,517 + +1.3 2,205,518 - -0.1 2,205,608 - -0.2 2,205,654 - +0.2 2,205,655 + -0.2 2,205,656 - -2.0 2,205,656 - +0.9 2,205,656 - -0.2 2,205,656 - -0.0 2,205,656 - +0.1 2,205,656 - -0.5 2,205,656 - +1.0 2,205,656 - -0.1 2,205,735 + -0.1 2,205,735 + +0.1 2,205,736 - -1.5 2,205,752 - -0.2 2,205,843 - Psest_2076 0.14 -1.8 2,206,123 - Psest_2076 0.49 -0.2 2,206,244 + Psest_2076 0.64 +1.1 2,206,245 - Psest_2076 0.64 -2.0 2,206,302 + Psest_2076 0.71 -0.3 2,206,302 + Psest_2076 0.71 -1.0 2,206,303 - Psest_2076 0.71 -0.1 2,206,303 - Psest_2076 0.71 +0.6 2,206,303 - Psest_2076 0.71 +0.8 2,206,303 - Psest_2076 0.71 +0.5 2,206,405 - Psest_2076 0.84 +0.3 2,206,406 + Psest_2076 0.84 -0.4 2,206,406 + Psest_2076 0.84 +0.8 2,206,407 - Psest_2076 0.84 -2.0 2,206,440 + Psest_2076 0.88 +0.4 2,206,440 + Psest_2076 0.88 -1.2 2,206,441 - Psest_2076 0.88 +0.7 2,206,505 + -0.3 2,206,505 + +0.2 2,206,506 - -0.9
Or see this region's nucleotide sequence