Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0184 and Psest_0185 are separated by 265 nucleotides Psest_0185 and Psest_0186 are separated by 183 nucleotides Psest_0186 and Psest_0187 are separated by 6 nucleotides
Psest_0184: Psest_0184 - ribosome biogenesis GTP-binding protein YsxC/EngB, at 180,007 to 180,660
_0184
Psest_0185: Psest_0185 - Cytochrome c553, at 180,926 to 181,558
_0185
Psest_0186: Psest_0186 - Protein-disulfide isomerase, at 181,742 to 182,371
_0186
Psest_0187: Psest_0187 - Metal-dependent hydrolase, at 182,378 to 183,247
_0187
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 180.008 kb on + strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.741 kb on - strand at 180.773 kb on + strand at 180.843 kb on + strand at 180.844 kb on - strand at 180.911 kb on + strand at 180.911 kb on + strand at 180.920 kb on + strand at 180.921 kb on - strand at 180.936 kb on + strand at 181.418 kb on + strand, within Psest_0185 at 181.419 kb on - strand, within Psest_0185 at 181.538 kb on + strand at 181.569 kb on - strand at 181.598 kb on + strand at 181.599 kb on - strand at 181.599 kb on - strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.832 kb on + strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.183 kb on + strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.211 kb on + strand, within Psest_0186 at 182.240 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.534 kb on + strand, within Psest_0187 at 182.534 kb on + strand, within Psest_0187
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 179,959 + +1.9 179,959 + -2.6 179,959 + -1.4 179,959 + -1.5 179,959 + -3.4 179,959 + +0.4 179,959 + +0.5 179,959 + -1.5 179,959 + +0.9 179,959 + -0.7 179,959 + -1.4 179,959 + -2.4 179,959 + -0.1 179,959 + -0.6 179,959 + -0.9 179,959 + -2.3 179,959 + -2.1 179,960 - +0.2 179,960 - -2.2 179,960 - +0.4 179,960 - +0.1 179,960 - +0.3 179,960 - +1.8 179,960 - +0.4 179,960 - +1.2 179,960 - +1.0 179,960 - -0.9 179,960 - -0.1 180,008 + -1.1 180,009 - +0.2 180,009 - +0.7 180,009 - +0.6 180,722 - +0.9 180,722 - +1.3 180,722 - -0.3 180,723 + -0.6 180,723 + -1.1 180,723 + -0.5 180,723 + -0.1 180,723 + -0.5 180,723 + -1.6 180,723 + +0.2 180,723 + -0.1 180,723 + -0.0 180,723 + -0.9 180,723 + -0.8 180,723 + -1.9 180,723 + -0.1 180,723 + +1.9 180,724 - +0.8 180,724 - -2.1 180,724 - +1.9 180,724 - -0.3 180,724 - +1.6 180,724 - +2.1 180,724 - +1.2 180,724 - +0.7 180,724 - +0.8 180,724 - +0.4 180,724 - +0.9 180,724 - +0.8 180,724 - -0.1 180,725 + +0.5 180,725 + +0.4 180,725 + -0.9 180,725 + -0.6 180,725 + +0.6 180,725 + -0.6 180,725 + -0.1 180,725 + -1.6 180,725 + -0.3 180,725 + -0.1 180,725 + -0.5 180,725 + +0.9 180,725 + -1.1 180,726 - +1.3 180,726 - +2.5 180,726 - +0.2 180,741 - +0.9 180,773 + -1.1 180,843 + -1.1 180,844 - +0.5 180,911 + +1.1 180,911 + +0.9 180,920 + +1.4 180,921 - +0.9 180,936 + +1.2 181,418 + Psest_0185 0.78 +2.2 181,419 - Psest_0185 0.78 +2.1 181,538 + -0.6 181,569 - +0.1 181,598 + +1.4 181,599 - -0.4 181,599 - -1.2 181,736 + +1.4 181,736 + -1.4 181,736 + -0.8 181,736 + +0.4 181,736 + -2.7 181,736 + -1.4 181,736 + -1.1 181,736 + -0.4 181,832 + Psest_0186 0.14 +0.9 181,833 - Psest_0186 0.14 -0.1 181,833 - Psest_0186 0.14 -0.1 181,833 - Psest_0186 0.14 -1.1 181,833 - Psest_0186 0.14 -0.1 182,016 + Psest_0186 0.43 -0.1 182,016 + Psest_0186 0.43 -2.1 182,017 - Psest_0186 0.44 -1.1 182,017 - Psest_0186 0.44 -2.1 182,017 - Psest_0186 0.44 -0.1 182,183 + Psest_0186 0.70 -2.4 182,184 - Psest_0186 0.70 -1.4 182,184 - Psest_0186 0.70 +1.5 182,211 + Psest_0186 0.74 +0.9 182,240 + Psest_0186 0.79 -1.4 182,282 + Psest_0186 0.86 -1.1 182,282 + Psest_0186 0.86 -0.1 182,282 + Psest_0186 0.86 -1.1 182,282 + Psest_0186 0.86 -1.6 182,282 + Psest_0186 0.86 -1.4 182,534 + Psest_0187 0.18 -1.4 182,534 + Psest_0187 0.18 -0.2
Or see this region's nucleotide sequence