Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0180 and Psest_0181 are separated by 212 nucleotides Psest_0181 and Psest_0182 are separated by 52 nucleotides Psest_0182 and Psest_0183 are separated by 59 nucleotides
Psest_0180: Psest_0180 - ABC-type Fe3+ transport system, permease component, at 173,534 to 175,150
_0180
Psest_0181: Psest_0181 - glycine cleavage system T protein, at 175,363 to 176,445
_0181
Psest_0182: Psest_0182 - glycine cleavage system H protein, at 176,498 to 176,887
_0182
Psest_0183: Psest_0183 - glycine dehydrogenase (decarboxylating), at 176,947 to 179,823
_0183
Position (kb) 175
176
177 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 175.266 kb on - strand at 175.313 kb on + strand at 175.374 kb on + strand at 175.374 kb on + strand at 175.375 kb on - strand at 175.384 kb on + strand at 175.463 kb on + strand at 175.463 kb on + strand at 175.463 kb on + strand at 175.463 kb on + strand at 175.464 kb on - strand at 175.555 kb on + strand, within Psest_0181 at 175.556 kb on - strand, within Psest_0181 at 175.556 kb on - strand, within Psest_0181 at 175.556 kb on - strand, within Psest_0181 at 175.556 kb on - strand, within Psest_0181 at 175.615 kb on + strand, within Psest_0181 at 175.615 kb on + strand, within Psest_0181 at 175.615 kb on + strand, within Psest_0181 at 175.667 kb on - strand, within Psest_0181 at 175.667 kb on - strand, within Psest_0181 at 175.924 kb on + strand, within Psest_0181 at 176.307 kb on + strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.435 kb on + strand at 176.435 kb on + strand at 176.436 kb on - strand at 176.436 kb on - strand at 176.443 kb on + strand at 176.443 kb on + strand at 176.444 kb on - strand at 176.444 kb on - strand at 176.622 kb on - strand, within Psest_0182 at 176.622 kb on - strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.786 kb on + strand, within Psest_0182 at 176.940 kb on - strand at 176.940 kb on - strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 177.278 kb on - strand, within Psest_0183 at 177.278 kb on - strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 175,266 - -0.1 175,313 + +1.1 175,374 + -2.4 175,374 + -3.2 175,375 - -1.9 175,384 + -0.7 175,463 + -4.2 175,463 + -4.1 175,463 + -3.5 175,463 + -2.8 175,464 - -2.4 175,555 + Psest_0181 0.18 -1.7 175,556 - Psest_0181 0.18 -4.2 175,556 - Psest_0181 0.18 -3.2 175,556 - Psest_0181 0.18 -4.5 175,556 - Psest_0181 0.18 -1.4 175,615 + Psest_0181 0.23 -1.7 175,615 + Psest_0181 0.23 -0.1 175,615 + Psest_0181 0.23 -4.8 175,667 - Psest_0181 0.28 -3.1 175,667 - Psest_0181 0.28 -3.7 175,924 + Psest_0181 0.52 -3.4 176,307 + Psest_0181 0.87 -1.6 176,308 - Psest_0181 0.87 -2.4 176,308 - Psest_0181 0.87 -3.4 176,308 - Psest_0181 0.87 -1.4 176,308 - Psest_0181 0.87 -2.9 176,308 - Psest_0181 0.87 -3.9 176,435 + -3.1 176,435 + -1.6 176,436 - -1.9 176,436 - -2.4 176,443 + -2.1 176,443 + -0.1 176,444 - -3.0 176,444 - -4.4 176,622 - Psest_0182 0.32 -2.4 176,622 - Psest_0182 0.32 -2.2 176,708 + Psest_0182 0.54 -0.1 176,708 + Psest_0182 0.54 -1.1 176,708 + Psest_0182 0.54 +1.7 176,708 + Psest_0182 0.54 +0.8 176,708 + Psest_0182 0.54 +1.5 176,708 + Psest_0182 0.54 -1.0 176,709 - Psest_0182 0.54 -2.8 176,709 - Psest_0182 0.54 -1.2 176,709 - Psest_0182 0.54 -4.1 176,709 - Psest_0182 0.54 -2.9 176,709 - Psest_0182 0.54 -2.9 176,709 - Psest_0182 0.54 -1.1 176,709 - Psest_0182 0.54 -4.2 176,709 - Psest_0182 0.54 -2.9 176,709 - Psest_0182 0.54 -1.4 176,786 + Psest_0182 0.74 -1.9 176,940 - +0.3 176,940 - +1.3 176,953 + -1.4 176,953 + -1.4 176,953 + -0.4 176,953 + -0.3 176,953 + -0.4 176,953 + -1.0 176,953 + -0.8 176,953 + -1.1 176,954 - +0.5 176,954 - -0.1 176,954 - -1.7 176,954 - +0.6 176,954 - +0.7 176,954 - +0.4 176,954 - +0.5 176,954 - +0.9 176,955 + -1.6 176,955 + -0.9 176,955 + +0.4 176,955 + -0.6 176,955 + -1.5 177,278 - Psest_0183 0.12 +0.8 177,278 - Psest_0183 0.12 +1.9 177,286 + Psest_0183 0.12 -0.8 177,286 + Psest_0183 0.12 +0.5 177,286 + Psest_0183 0.12 +0.2 177,286 + Psest_0183 0.12 -1.2 177,287 - Psest_0183 0.12 +1.0 177,287 - Psest_0183 0.12 -0.1 177,341 - Psest_0183 0.14 -0.9 177,341 - Psest_0183 0.14 +0.4 177,341 - Psest_0183 0.14 +0.5 177,341 - Psest_0183 0.14 +0.2
Or see this region's nucleotide sequence