Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1623 and Psest_1624 are separated by 28 nucleotides Psest_1624 and Psest_1625 are separated by 57 nucleotides Psest_1625 and Psest_1626 are separated by 45 nucleotides Psest_1626 and Psest_1627 are separated by 150 nucleotides
Psest_1623: Psest_1623 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 1,726,741 to 1,727,295
_1623
Psest_1624: Psest_1624 - Transcriptional regulator, at 1,727,324 to 1,728,232
_1624
Psest_1625: Psest_1625 - hypothetical protein, at 1,728,290 to 1,728,610
_1625
Psest_1626: Psest_1626 - Predicted integral membrane protein, at 1,728,656 to 1,729,093
_1626
Psest_1627: Psest_1627 - Superfamily II DNA and RNA helicases, at 1,729,244 to 1,730,575
_1627
Position (kb)
1728
1729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1727.338 kb on + strand at 1727.338 kb on + strand at 1727.339 kb on - strand at 1727.358 kb on + strand at 1727.358 kb on + strand at 1727.359 kb on - strand at 1727.416 kb on + strand, within Psest_1624 at 1727.421 kb on + strand, within Psest_1624 at 1727.421 kb on + strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.117 kb on + strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.118 kb on - strand, within Psest_1624 at 1728.246 kb on - strand at 1728.248 kb on - strand at 1728.272 kb on - strand at 1728.351 kb on - strand, within Psest_1625 at 1728.357 kb on + strand, within Psest_1625 at 1728.358 kb on - strand, within Psest_1625 at 1728.447 kb on - strand, within Psest_1625 at 1728.608 kb on + strand at 1728.609 kb on - strand at 1728.665 kb on + strand at 1728.666 kb on - strand at 1728.666 kb on - strand at 1728.744 kb on + strand, within Psest_1626 at 1728.754 kb on - strand, within Psest_1626 at 1728.933 kb on + strand, within Psest_1626 at 1729.038 kb on + strand, within Psest_1626 at 1729.039 kb on - strand, within Psest_1626 at 1729.039 kb on - strand, within Psest_1626 at 1729.055 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.206 kb on + strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.207 kb on - strand at 1729.216 kb on + strand at 1729.217 kb on - strand at 1729.236 kb on + strand at 1729.237 kb on - strand at 1729.362 kb on + strand at 1729.362 kb on + strand at 1729.362 kb on + strand at 1729.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 1,727,338 + +0.3 1,727,338 + -2.7 1,727,339 - -1.7 1,727,358 + -0.8 1,727,358 + -0.3 1,727,359 - -1.4 1,727,416 + Psest_1624 0.10 +1.8 1,727,421 + Psest_1624 0.11 +0.7 1,727,421 + Psest_1624 0.11 -0.3 1,727,422 - Psest_1624 0.11 -0.5 1,727,422 - Psest_1624 0.11 +0.8 1,727,422 - Psest_1624 0.11 -0.3 1,727,427 + Psest_1624 0.11 +1.9 1,727,427 + Psest_1624 0.11 +0.3 1,727,427 + Psest_1624 0.11 +1.2 1,727,427 + Psest_1624 0.11 +0.8 1,727,427 + Psest_1624 0.11 -0.6 1,727,427 + Psest_1624 0.11 -0.3 1,727,428 - Psest_1624 0.11 +1.7 1,727,428 - Psest_1624 0.11 +0.4 1,727,428 - Psest_1624 0.11 -0.9 1,728,117 + Psest_1624 0.87 -0.9 1,728,117 + Psest_1624 0.87 +0.1 1,728,117 + Psest_1624 0.87 +0.3 1,728,117 + Psest_1624 0.87 -0.7 1,728,117 + Psest_1624 0.87 -2.7 1,728,117 + Psest_1624 0.87 -0.8 1,728,118 - Psest_1624 0.87 -1.8 1,728,118 - Psest_1624 0.87 +1.2 1,728,118 - Psest_1624 0.87 -0.9 1,728,118 - Psest_1624 0.87 -0.2 1,728,118 - Psest_1624 0.87 +1.0 1,728,118 - Psest_1624 0.87 +0.2 1,728,246 - -1.2 1,728,248 - +1.4 1,728,272 - +0.1 1,728,351 - Psest_1625 0.19 +1.2 1,728,357 + Psest_1625 0.21 -3.2 1,728,358 - Psest_1625 0.21 -2.0 1,728,447 - Psest_1625 0.49 -1.4 1,728,608 + +1.2 1,728,609 - -1.0 1,728,665 + +0.3 1,728,666 - -1.1 1,728,666 - -0.8 1,728,744 + Psest_1626 0.20 +0.4 1,728,754 - Psest_1626 0.22 -1.3 1,728,933 + Psest_1626 0.63 -1.1 1,729,038 + Psest_1626 0.87 -1.2 1,729,039 - Psest_1626 0.87 -0.2 1,729,039 - Psest_1626 0.87 -2.6 1,729,055 + -0.1 1,729,206 + -2.9 1,729,206 + -0.6 1,729,206 + -2.2 1,729,206 + +0.0 1,729,207 - +1.0 1,729,207 - -1.5 1,729,207 - +0.6 1,729,207 - -2.6 1,729,207 - -0.3 1,729,216 + +0.2 1,729,217 - -1.0 1,729,236 + -0.5 1,729,237 - -2.2 1,729,362 + -1.2 1,729,362 + -1.3 1,729,362 + -2.7 1,729,363 - -1.0
Or see this region's nucleotide sequence