Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0121 and Psest_0122 are separated by 5 nucleotides Psest_0122 and Psest_0123 overlap by 28 nucleotides Psest_0123 and Psest_0124 are separated by 15 nucleotides
Psest_0121: Psest_0121 - LysM domain., at 116,322 to 117,569
_0121
Psest_0122: Psest_0122 - hypothetical protein, at 117,575 to 118,429
_0122
Psest_0123: Psest_0123 - Rhs element Vgr protein, at 118,402 to 120,501
_0123
Psest_0124: Psest_0124 - Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains, at 120,517 to 122,034
_0124
Position (kb)
118
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 117.562 kb on + strand at 117.562 kb on + strand at 117.563 kb on - strand at 117.563 kb on - strand at 117.563 kb on - strand at 117.563 kb on - strand at 117.659 kb on + strand at 117.659 kb on + strand at 117.660 kb on - strand at 117.660 kb on - strand at 117.660 kb on - strand at 117.660 kb on - strand at 117.708 kb on + strand, within Psest_0122 at 117.708 kb on + strand, within Psest_0122 at 117.709 kb on - strand, within Psest_0122 at 117.831 kb on - strand, within Psest_0122 at 118.068 kb on + strand, within Psest_0122 at 118.068 kb on + strand, within Psest_0122 at 118.069 kb on - strand, within Psest_0122 at 118.069 kb on - strand, within Psest_0122 at 118.230 kb on + strand, within Psest_0122 at 118.230 kb on + strand, within Psest_0122 at 118.230 kb on + strand, within Psest_0122 at 118.231 kb on - strand, within Psest_0122 at 118.279 kb on - strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.314 kb on + strand, within Psest_0122 at 118.315 kb on - strand, within Psest_0122 at 118.315 kb on - strand, within Psest_0122 at 118.315 kb on - strand, within Psest_0122 at 118.359 kb on + strand at 118.360 kb on - strand at 118.360 kb on - strand at 118.360 kb on - strand at 118.360 kb on - strand at 118.391 kb on + strand at 118.391 kb on + strand at 118.391 kb on + strand at 118.391 kb on + strand at 118.391 kb on + strand at 118.392 kb on - strand at 118.392 kb on - strand at 118.392 kb on - strand at 118.392 kb on - strand at 118.392 kb on - strand at 118.392 kb on - strand at 118.589 kb on - strand at 118.757 kb on + strand, within Psest_0123 at 118.757 kb on + strand, within Psest_0123 at 118.757 kb on + strand, within Psest_0123 at 118.757 kb on + strand, within Psest_0123 at 118.757 kb on + strand, within Psest_0123 at 118.758 kb on - strand, within Psest_0123 at 118.758 kb on - strand, within Psest_0123 at 118.758 kb on - strand, within Psest_0123 at 118.818 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.907 kb on + strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 118.908 kb on - strand, within Psest_0123 at 119.003 kb on + strand, within Psest_0123 at 119.003 kb on + strand, within Psest_0123 at 119.003 kb on + strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.004 kb on - strand, within Psest_0123 at 119.138 kb on + strand, within Psest_0123 at 119.539 kb on - strand, within Psest_0123 at 119.539 kb on - strand, within Psest_0123 at 119.539 kb on - strand, within Psest_0123 at 119.744 kb on + strand, within Psest_0123 at 119.817 kb on - strand, within Psest_0123 at 119.912 kb on + strand, within Psest_0123 at 119.996 kb on + strand, within Psest_0123 at 119.996 kb on + strand, within Psest_0123 at 120.053 kb on + strand, within Psest_0123 at 120.053 kb on + strand, within Psest_0123 at 120.065 kb on + strand, within Psest_0123 at 120.065 kb on + strand, within Psest_0123 at 120.207 kb on - strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.280 kb on + strand, within Psest_0123 at 120.281 kb on - strand, within Psest_0123 at 120.519 kb on - strand at 120.519 kb on - strand at 120.519 kb on - strand at 120.519 kb on - strand at 120.715 kb on + strand, within Psest_0124 at 120.715 kb on + strand, within Psest_0124 at 120.715 kb on + strand, within Psest_0124 at 120.715 kb on + strand, within Psest_0124 at 120.715 kb on + strand, within Psest_0124 at 120.715 kb on + strand, within Psest_0124 at 120.716 kb on - strand, within Psest_0124 at 120.716 kb on - strand, within Psest_0124 at 120.716 kb on - strand, within Psest_0124 at 120.716 kb on - strand, within Psest_0124 at 120.716 kb on - strand, within Psest_0124 at 120.962 kb on + strand, within Psest_0124 at 121.276 kb on + strand, within Psest_0124 at 121.276 kb on + strand, within Psest_0124 at 121.407 kb on - strand, within Psest_0124
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 117,562 + -0.6 117,562 + -1.9 117,563 - +1.6 117,563 - -0.3 117,563 - -0.1 117,563 - -0.1 117,659 + -1.7 117,659 + -0.3 117,660 - +0.1 117,660 - -0.8 117,660 - +0.1 117,660 - +0.7 117,708 + Psest_0122 0.16 +1.9 117,708 + Psest_0122 0.16 -0.6 117,709 - Psest_0122 0.16 -1.1 117,831 - Psest_0122 0.30 -0.9 118,068 + Psest_0122 0.58 -1.1 118,068 + Psest_0122 0.58 -2.3 118,069 - Psest_0122 0.58 +1.9 118,069 - Psest_0122 0.58 -0.7 118,230 + Psest_0122 0.77 -0.7 118,230 + Psest_0122 0.77 +0.1 118,230 + Psest_0122 0.77 +0.1 118,231 - Psest_0122 0.77 -1.6 118,279 - Psest_0122 0.82 -0.5 118,314 + Psest_0122 0.86 -0.3 118,314 + Psest_0122 0.86 -0.1 118,314 + Psest_0122 0.86 -0.1 118,314 + Psest_0122 0.86 -1.1 118,314 + Psest_0122 0.86 -1.1 118,314 + Psest_0122 0.86 -0.3 118,315 - Psest_0122 0.87 -0.4 118,315 - Psest_0122 0.87 +0.5 118,315 - Psest_0122 0.87 -0.7 118,359 + +1.4 118,360 - -0.5 118,360 - +0.7 118,360 - -1.4 118,360 - -0.3 118,391 + -0.4 118,391 + +1.0 118,391 + +1.9 118,391 + -1.1 118,391 + -0.6 118,392 - +0.2 118,392 - -0.1 118,392 - +0.1 118,392 - -0.4 118,392 - -2.7 118,392 - +0.9 118,589 - +0.3 118,757 + Psest_0123 0.17 +0.5 118,757 + Psest_0123 0.17 +0.9 118,757 + Psest_0123 0.17 -0.2 118,757 + Psest_0123 0.17 -2.4 118,757 + Psest_0123 0.17 +1.1 118,758 - Psest_0123 0.17 +2.2 118,758 - Psest_0123 0.17 -1.1 118,758 - Psest_0123 0.17 +1.3 118,818 + Psest_0123 0.20 -0.1 118,907 + Psest_0123 0.24 +0.3 118,907 + Psest_0123 0.24 -0.2 118,907 + Psest_0123 0.24 +1.1 118,907 + Psest_0123 0.24 -0.1 118,907 + Psest_0123 0.24 -0.1 118,907 + Psest_0123 0.24 +0.9 118,907 + Psest_0123 0.24 -1.7 118,908 - Psest_0123 0.24 -2.0 118,908 - Psest_0123 0.24 -0.7 118,908 - Psest_0123 0.24 -1.4 118,908 - Psest_0123 0.24 -1.3 118,908 - Psest_0123 0.24 -0.7 118,908 - Psest_0123 0.24 -2.1 118,908 - Psest_0123 0.24 -1.1 119,003 + Psest_0123 0.29 -0.1 119,003 + Psest_0123 0.29 -0.1 119,003 + Psest_0123 0.29 -0.1 119,004 - Psest_0123 0.29 -1.9 119,004 - Psest_0123 0.29 -1.0 119,004 - Psest_0123 0.29 -0.7 119,004 - Psest_0123 0.29 -1.6 119,004 - Psest_0123 0.29 -3.3 119,004 - Psest_0123 0.29 -0.7 119,004 - Psest_0123 0.29 -1.4 119,004 - Psest_0123 0.29 -0.7 119,138 + Psest_0123 0.35 -0.6 119,539 - Psest_0123 0.54 -1.5 119,539 - Psest_0123 0.54 -1.0 119,539 - Psest_0123 0.54 -2.0 119,744 + Psest_0123 0.64 +0.9 119,817 - Psest_0123 0.67 -1.2 119,912 + Psest_0123 0.72 -1.3 119,996 + Psest_0123 0.76 +0.3 119,996 + Psest_0123 0.76 -0.3 120,053 + Psest_0123 0.79 +0.4 120,053 + Psest_0123 0.79 +0.3 120,065 + Psest_0123 0.79 -1.0 120,065 + Psest_0123 0.79 +0.2 120,207 - Psest_0123 0.86 -0.1 120,280 + Psest_0123 0.89 +1.2 120,280 + Psest_0123 0.89 +2.2 120,280 + Psest_0123 0.89 -0.4 120,280 + Psest_0123 0.89 -0.1 120,280 + Psest_0123 0.89 +0.1 120,280 + Psest_0123 0.89 +0.9 120,280 + Psest_0123 0.89 -0.7 120,280 + Psest_0123 0.89 +0.6 120,280 + Psest_0123 0.89 -0.5 120,281 - Psest_0123 0.89 -1.9 120,519 - +2.9 120,519 - -0.1 120,519 - -2.9 120,519 - -2.6 120,715 + Psest_0124 0.13 -0.1 120,715 + Psest_0124 0.13 +0.5 120,715 + Psest_0124 0.13 +0.9 120,715 + Psest_0124 0.13 -0.7 120,715 + Psest_0124 0.13 -2.6 120,715 + Psest_0124 0.13 -0.3 120,716 - Psest_0124 0.13 -0.5 120,716 - Psest_0124 0.13 +0.3 120,716 - Psest_0124 0.13 +0.3 120,716 - Psest_0124 0.13 +0.2 120,716 - Psest_0124 0.13 -2.4 120,962 + Psest_0124 0.29 -0.1 121,276 + Psest_0124 0.50 +1.3 121,276 + Psest_0124 0.50 -0.2 121,407 - Psest_0124 0.59 -0.7
Or see this region's nucleotide sequence