Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0010 and Psest_0011 overlap by 4 nucleotides Psest_0011 and Psest_0012 are separated by 107 nucleotides Psest_0012 and Psest_0013 are separated by 108 nucleotides
Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410
_0010
Psest_0011: Psest_0011 - glycyl-tRNA synthetase, tetrameric type, alpha subunit, at 13,407 to 14,354
_0011
Psest_0012: Psest_0012 - DNA-3-methyladenine glycosylase I, at 14,462 to 15,019
_0012
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 14.395 kb on - strand at 14.395 kb on - strand at 14.395 kb on - strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.499 kb on - strand at 14.499 kb on - strand at 14.936 kb on + strand, within Psest_0012 at 14.936 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.994 kb on - strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.034 kb on - strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 14,395 - +1.6 14,395 - +0.1 14,395 - +2.2 14,498 + -1.0 14,498 + +0.2 14,498 + +0.2 14,498 + +0.5 14,498 + +0.4 14,499 - -0.1 14,499 - +0.8 14,936 + Psest_0012 0.85 +0.1 14,936 + Psest_0012 0.85 -2.3 14,942 + Psest_0012 0.86 -0.7 14,942 + Psest_0012 0.86 +0.2 14,942 + Psest_0012 0.86 +0.4 14,942 + Psest_0012 0.86 -0.4 14,942 + Psest_0012 0.86 -0.1 14,942 + Psest_0012 0.86 -0.2 14,942 + Psest_0012 0.86 +0.2 14,942 + Psest_0012 0.86 -0.5 14,942 + Psest_0012 0.86 +0.6 14,942 + Psest_0012 0.86 -0.1 14,942 + Psest_0012 0.86 -0.6 14,942 + Psest_0012 0.86 +0.5 14,943 - Psest_0012 0.86 +1.7 14,943 - Psest_0012 0.86 -0.2 14,943 - Psest_0012 0.86 -0.6 14,943 - Psest_0012 0.86 +0.1 14,943 - Psest_0012 0.86 -0.5 14,943 - Psest_0012 0.86 -0.5 14,994 - -1.2 15,033 + +0.9 15,033 + +0.6 15,033 + -0.1 15,034 - -0.7 15,094 + -0.7 15,094 + -0.3 15,094 + -0.2 15,095 - +0.9
Or see this region's nucleotide sequence