Experiment: Def Benzalkonium Chloride 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1114 and Psest_1115 are separated by 21 nucleotides Psest_1115 and Psest_1116 overlap by 8 nucleotides Psest_1116 and Psest_1117 are separated by 84 nucleotides Psest_1117 and Psest_1118 are separated by 57 nucleotides
Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767
_1114
Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538
_1115
Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325
_1116
Psest_1117: Psest_1117 - Lipid A 3-O-deacylase (PagL)., at 1,211,410 to 1,211,928
_1117
Psest_1118: Psest_1118 - Predicted Fe-S-cluster oxidoreductase, at 1,211,986 to 1,212,297
_1118
Position (kb)
1210
1211
1212 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1209.927 kb on + strand, within Psest_1115 at 1209.927 kb on + strand, within Psest_1115 at 1209.974 kb on + strand, within Psest_1115 at 1210.340 kb on + strand, within Psest_1115 at 1211.395 kb on + strand at 1211.395 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.398 kb on - strand at 1211.398 kb on - strand at 1211.617 kb on + strand, within Psest_1117 at 1211.618 kb on - strand, within Psest_1117 at 1211.677 kb on + strand, within Psest_1117 at 1211.677 kb on + strand, within Psest_1117 at 1211.678 kb on - strand, within Psest_1117 at 1211.707 kb on + strand, within Psest_1117 at 1211.707 kb on + strand, within Psest_1117 at 1211.707 kb on + strand, within Psest_1117 at 1211.745 kb on + strand, within Psest_1117 at 1211.855 kb on - strand, within Psest_1117 at 1211.926 kb on + strand at 1211.926 kb on + strand at 1211.926 kb on + strand at 1212.077 kb on + strand, within Psest_1118 at 1212.078 kb on - strand, within Psest_1118 at 1212.078 kb on - strand, within Psest_1118 at 1212.315 kb on + strand at 1212.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Benzalkonium Chloride 0.002 mg/ml remove 1,209,927 + Psest_1115 0.18 -3.0 1,209,927 + Psest_1115 0.18 -3.9 1,209,974 + Psest_1115 0.25 -2.8 1,210,340 + Psest_1115 0.73 -2.0 1,211,395 + +1.2 1,211,395 + +1.2 1,211,397 + +1.2 1,211,397 + +1.9 1,211,397 + +0.0 1,211,398 - +2.1 1,211,398 - -0.9 1,211,617 + Psest_1117 0.40 +1.0 1,211,618 - Psest_1117 0.40 -3.9 1,211,677 + Psest_1117 0.51 -0.1 1,211,677 + Psest_1117 0.51 -0.1 1,211,678 - Psest_1117 0.52 -2.6 1,211,707 + Psest_1117 0.57 -0.7 1,211,707 + Psest_1117 0.57 +0.2 1,211,707 + Psest_1117 0.57 -0.5 1,211,745 + Psest_1117 0.65 -0.8 1,211,855 - Psest_1117 0.86 -2.4 1,211,926 + +0.7 1,211,926 + +1.5 1,211,926 + +0.8 1,212,077 + Psest_1118 0.29 -0.8 1,212,078 - Psest_1118 0.29 -1.6 1,212,078 - Psest_1118 0.29 +0.2 1,212,315 + +0.9 1,212,315 + +0.6
Or see this region's nucleotide sequence