Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1020 and Psest_1021 are separated by 78 nucleotides Psest_1021 and Psest_1022 are separated by 213 nucleotides Psest_1022 and Psest_1023 are separated by 22 nucleotides Psest_1023 and Psest_1024 are separated by 133 nucleotides
Psest_1020: Psest_1020 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 1,107,066 to 1,107,521
_1020
Psest_1021: Psest_1021 - 3-dehydroquinate dehydratase, type II, at 1,107,600 to 1,108,043
_1021
Psest_1022: Psest_1022 - Thiol:disulfide interchange protein, at 1,108,257 to 1,110,068
_1022
Psest_1023: Psest_1023 - Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases, at 1,110,091 to 1,110,426
_1023
Psest_1024: Psest_1024 - ABC-type Fe3+-siderophore transport system, permease component, at 1,110,560 to 1,111,597
_1024
Position (kb)
1108
1109
1110
1111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1108.172 kb on - strand at 1108.308 kb on + strand at 1108.321 kb on - strand at 1108.343 kb on + strand at 1108.391 kb on - strand at 1108.446 kb on + strand, within Psest_1022 at 1108.454 kb on - strand, within Psest_1022 at 1108.534 kb on + strand, within Psest_1022 at 1108.535 kb on - strand, within Psest_1022 at 1108.726 kb on + strand, within Psest_1022 at 1108.737 kb on + strand, within Psest_1022 at 1108.738 kb on - strand, within Psest_1022 at 1108.863 kb on + strand, within Psest_1022 at 1108.911 kb on + strand, within Psest_1022 at 1108.911 kb on + strand, within Psest_1022 at 1108.911 kb on + strand, within Psest_1022 at 1108.912 kb on - strand, within Psest_1022 at 1108.912 kb on - strand, within Psest_1022 at 1109.059 kb on + strand, within Psest_1022 at 1109.059 kb on + strand, within Psest_1022 at 1109.059 kb on + strand, within Psest_1022 at 1109.060 kb on - strand, within Psest_1022 at 1109.060 kb on - strand, within Psest_1022 at 1109.060 kb on - strand, within Psest_1022 at 1109.060 kb on - strand, within Psest_1022 at 1109.244 kb on + strand, within Psest_1022 at 1109.367 kb on + strand, within Psest_1022 at 1109.367 kb on + strand, within Psest_1022 at 1109.367 kb on + strand, within Psest_1022 at 1109.368 kb on - strand, within Psest_1022 at 1109.368 kb on - strand, within Psest_1022 at 1109.368 kb on - strand, within Psest_1022 at 1109.371 kb on + strand, within Psest_1022 at 1109.371 kb on + strand, within Psest_1022 at 1109.371 kb on + strand, within Psest_1022 at 1109.371 kb on + strand, within Psest_1022 at 1109.371 kb on + strand, within Psest_1022 at 1109.372 kb on - strand, within Psest_1022 at 1109.372 kb on - strand, within Psest_1022 at 1109.422 kb on - strand, within Psest_1022 at 1109.707 kb on + strand, within Psest_1022 at 1109.707 kb on + strand, within Psest_1022 at 1109.708 kb on - strand, within Psest_1022 at 1109.708 kb on - strand, within Psest_1022 at 1109.708 kb on - strand, within Psest_1022 at 1109.708 kb on - strand, within Psest_1022 at 1109.708 kb on - strand, within Psest_1022 at 1109.824 kb on + strand, within Psest_1022 at 1109.824 kb on + strand, within Psest_1022 at 1109.824 kb on + strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.825 kb on - strand, within Psest_1022 at 1109.827 kb on + strand, within Psest_1022 at 1109.827 kb on + strand, within Psest_1022 at 1109.828 kb on - strand, within Psest_1022 at 1110.044 kb on - strand at 1110.062 kb on + strand at 1110.317 kb on + strand, within Psest_1023 at 1110.416 kb on - strand at 1110.538 kb on + strand at 1110.950 kb on + strand, within Psest_1024 at 1110.950 kb on + strand, within Psest_1024 at 1110.950 kb on + strand, within Psest_1024 at 1110.951 kb on - strand, within Psest_1024 at 1111.005 kb on - strand, within Psest_1024
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 1,108,172 - +1.1 1,108,308 + -2.1 1,108,321 - +0.1 1,108,343 + -1.0 1,108,391 - -0.1 1,108,446 + Psest_1022 0.10 +0.3 1,108,454 - Psest_1022 0.11 -2.3 1,108,534 + Psest_1022 0.15 -1.0 1,108,535 - Psest_1022 0.15 -0.2 1,108,726 + Psest_1022 0.26 -1.0 1,108,737 + Psest_1022 0.26 +0.1 1,108,738 - Psest_1022 0.27 -2.7 1,108,863 + Psest_1022 0.33 +0.9 1,108,911 + Psest_1022 0.36 +1.7 1,108,911 + Psest_1022 0.36 -0.7 1,108,911 + Psest_1022 0.36 -1.2 1,108,912 - Psest_1022 0.36 -1.7 1,108,912 - Psest_1022 0.36 +0.5 1,109,059 + Psest_1022 0.44 -0.8 1,109,059 + Psest_1022 0.44 -0.1 1,109,059 + Psest_1022 0.44 -3.2 1,109,060 - Psest_1022 0.44 -0.7 1,109,060 - Psest_1022 0.44 -0.8 1,109,060 - Psest_1022 0.44 +0.9 1,109,060 - Psest_1022 0.44 +0.1 1,109,244 + Psest_1022 0.54 -2.4 1,109,367 + Psest_1022 0.61 +0.3 1,109,367 + Psest_1022 0.61 -1.4 1,109,367 + Psest_1022 0.61 +0.9 1,109,368 - Psest_1022 0.61 -1.4 1,109,368 - Psest_1022 0.61 -1.5 1,109,368 - Psest_1022 0.61 +0.9 1,109,371 + Psest_1022 0.61 +0.1 1,109,371 + Psest_1022 0.61 +0.5 1,109,371 + Psest_1022 0.61 -0.8 1,109,371 + Psest_1022 0.61 -0.1 1,109,371 + Psest_1022 0.61 -1.9 1,109,372 - Psest_1022 0.62 +0.9 1,109,372 - Psest_1022 0.62 -0.1 1,109,422 - Psest_1022 0.64 -0.8 1,109,707 + Psest_1022 0.80 -0.8 1,109,707 + Psest_1022 0.80 -1.3 1,109,708 - Psest_1022 0.80 -0.7 1,109,708 - Psest_1022 0.80 -1.1 1,109,708 - Psest_1022 0.80 -0.5 1,109,708 - Psest_1022 0.80 -0.9 1,109,708 - Psest_1022 0.80 +0.3 1,109,824 + Psest_1022 0.86 -3.5 1,109,824 + Psest_1022 0.86 -1.7 1,109,824 + Psest_1022 0.86 -1.4 1,109,825 - Psest_1022 0.87 -1.5 1,109,825 - Psest_1022 0.87 -2.7 1,109,825 - Psest_1022 0.87 -2.1 1,109,825 - Psest_1022 0.87 -0.7 1,109,825 - Psest_1022 0.87 -1.4 1,109,825 - Psest_1022 0.87 +1.7 1,109,825 - Psest_1022 0.87 -0.2 1,109,827 + Psest_1022 0.87 -2.8 1,109,827 + Psest_1022 0.87 -2.3 1,109,828 - Psest_1022 0.87 -0.8 1,110,044 - -0.2 1,110,062 + -0.9 1,110,317 + Psest_1023 0.67 +0.6 1,110,416 - +0.9 1,110,538 + +2.3 1,110,950 + Psest_1024 0.38 -1.4 1,110,950 + Psest_1024 0.38 +0.5 1,110,950 + Psest_1024 0.38 +0.2 1,110,951 - Psest_1024 0.38 -1.1 1,111,005 - Psest_1024 0.43 +0.7
Or see this region's nucleotide sequence