Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0957 and Psest_0958 are separated by 210 nucleotides Psest_0958 and Psest_0959 overlap by 8 nucleotides Psest_0959 and Psest_0960 are separated by 40 nucleotides Psest_0960 and Psest_0961 are separated by 95 nucleotides Psest_0961 and Psest_0962 are separated by 58 nucleotides
Psest_0957: Psest_0957 - SsrA-binding protein, at 1,039,260 to 1,039,742
_0957
Psest_0958: Psest_0958 - Oligoketide cyclase/lipid transport protein, at 1,039,953 to 1,040,387
_0958
Psest_0959: Psest_0959 - Uncharacterized protein conserved in bacteria, at 1,040,380 to 1,040,694
_0959
Psest_0960: Psest_0960 - Small protein A (tmRNA-binding), at 1,040,735 to 1,041,250
_0960
Psest_0961: Psest_0961 - Fe2+/Zn2+ uptake regulation proteins, at 1,041,346 to 1,041,750
_0961
Psest_0962: Psest_0962 - DNA repair protein RecN, at 1,041,809 to 1,043,482
_0962
Position (kb)
1040
1041
1042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1039.755 kb on - strand at 1039.757 kb on - strand at 1039.757 kb on - strand at 1039.757 kb on - strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.941 kb on + strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1039.942 kb on - strand at 1040.026 kb on + strand, within Psest_0958 at 1040.026 kb on + strand, within Psest_0958 at 1040.027 kb on - strand, within Psest_0958 at 1040.027 kb on - strand, within Psest_0958 at 1040.081 kb on + strand, within Psest_0958 at 1040.082 kb on - strand, within Psest_0958 at 1040.205 kb on - strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.212 kb on + strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.213 kb on - strand, within Psest_0958 at 1040.379 kb on + strand at 1040.379 kb on + strand at 1040.379 kb on + strand at 1040.380 kb on - strand at 1040.413 kb on + strand, within Psest_0959 at 1040.413 kb on + strand, within Psest_0959 at 1040.467 kb on - strand, within Psest_0959 at 1040.561 kb on + strand, within Psest_0959 at 1040.611 kb on + strand, within Psest_0959 at 1040.612 kb on - strand, within Psest_0959 at 1042.004 kb on + strand, within Psest_0962 at 1042.022 kb on + strand, within Psest_0962 at 1042.022 kb on + strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.023 kb on - strand, within Psest_0962 at 1042.109 kb on + strand, within Psest_0962 at 1042.110 kb on - strand, within Psest_0962 at 1042.110 kb on - strand, within Psest_0962 at 1042.173 kb on - strand, within Psest_0962 at 1042.173 kb on - strand, within Psest_0962
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 1,039,755 - -0.3 1,039,757 - -0.1 1,039,757 - -0.1 1,039,757 - -1.2 1,039,941 + -0.4 1,039,941 + -0.9 1,039,941 + -0.1 1,039,941 + +1.2 1,039,941 + -0.0 1,039,941 + -0.3 1,039,941 + +0.6 1,039,941 + +1.2 1,039,942 - -0.6 1,039,942 - -0.9 1,039,942 - +0.4 1,039,942 - -0.1 1,040,026 + Psest_0958 0.17 -0.4 1,040,026 + Psest_0958 0.17 +0.3 1,040,027 - Psest_0958 0.17 -1.4 1,040,027 - Psest_0958 0.17 -1.1 1,040,081 + Psest_0958 0.29 -1.5 1,040,082 - Psest_0958 0.30 -0.4 1,040,205 - Psest_0958 0.58 +0.3 1,040,212 + Psest_0958 0.60 +0.2 1,040,212 + Psest_0958 0.60 +0.9 1,040,212 + Psest_0958 0.60 -1.9 1,040,212 + Psest_0958 0.60 -1.2 1,040,212 + Psest_0958 0.60 -1.8 1,040,212 + Psest_0958 0.60 +0.1 1,040,212 + Psest_0958 0.60 -0.9 1,040,212 + Psest_0958 0.60 -0.8 1,040,212 + Psest_0958 0.60 -1.1 1,040,212 + Psest_0958 0.60 -0.1 1,040,212 + Psest_0958 0.60 -0.8 1,040,212 + Psest_0958 0.60 +0.1 1,040,212 + Psest_0958 0.60 +2.9 1,040,212 + Psest_0958 0.60 -0.7 1,040,212 + Psest_0958 0.60 +1.2 1,040,212 + Psest_0958 0.60 +0.0 1,040,212 + Psest_0958 0.60 +0.5 1,040,213 - Psest_0958 0.60 -1.2 1,040,213 - Psest_0958 0.60 -0.2 1,040,213 - Psest_0958 0.60 -0.3 1,040,213 - Psest_0958 0.60 -1.4 1,040,213 - Psest_0958 0.60 -0.0 1,040,213 - Psest_0958 0.60 +0.6 1,040,213 - Psest_0958 0.60 -1.5 1,040,213 - Psest_0958 0.60 +0.3 1,040,213 - Psest_0958 0.60 -0.9 1,040,213 - Psest_0958 0.60 -0.9 1,040,213 - Psest_0958 0.60 -0.1 1,040,213 - Psest_0958 0.60 -0.5 1,040,213 - Psest_0958 0.60 -0.7 1,040,379 + +0.0 1,040,379 + +0.4 1,040,379 + +0.3 1,040,380 - -0.5 1,040,413 + Psest_0959 0.10 +0.2 1,040,413 + Psest_0959 0.10 +1.2 1,040,467 - Psest_0959 0.28 +2.6 1,040,561 + Psest_0959 0.57 +1.3 1,040,611 + Psest_0959 0.73 -0.6 1,040,612 - Psest_0959 0.74 +0.7 1,042,004 + Psest_0962 0.12 -1.1 1,042,022 + Psest_0962 0.13 -0.3 1,042,022 + Psest_0962 0.13 -3.0 1,042,023 - Psest_0962 0.13 -1.0 1,042,023 - Psest_0962 0.13 -2.1 1,042,023 - Psest_0962 0.13 -1.4 1,042,109 + Psest_0962 0.18 -0.8 1,042,110 - Psest_0962 0.18 -1.8 1,042,110 - Psest_0962 0.18 -2.6 1,042,173 - Psest_0962 0.22 -1.1 1,042,173 - Psest_0962 0.22 -2.2
Or see this region's nucleotide sequence