Experiment: Def Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0778 and Psest_0779 are separated by 195 nucleotides Psest_0779 and Psest_0780 overlap by 1 nucleotides Psest_0780 and Psest_0781 are separated by 227 nucleotides
Psest_0778: Psest_0778 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 846,878 to 847,639
_0778
Psest_0779: Psest_0779 - tyrosine aminotransferase (EC 2.6.1.57) <small>(from data)</small>, at 847,835 to 849,031
_0779
Psest_0780: Psest_0780 - Pterin-4a-carbinolamine dehydratase, at 849,031 to 849,387
_0780
Psest_0781: Psest_0781 - phenylalanine-4-hydroxylase, monomeric form, at 849,615 to 850,400
_0781
Position (kb) 847
848
849
850 Strain fitness (log2 ratio) -2
-1
0
1
2 at 846.885 kb on + strand at 846.885 kb on + strand at 846.973 kb on - strand, within Psest_0778 at 846.973 kb on - strand, within Psest_0778 at 847.020 kb on - strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.231 kb on - strand, within Psest_0778 at 847.375 kb on + strand, within Psest_0778 at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.836 kb on + strand at 847.836 kb on + strand at 847.837 kb on - strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.930 kb on - strand at 847.959 kb on - strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.984 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779 at 848.835 kb on + strand, within Psest_0779 at 848.835 kb on + strand, within Psest_0779 at 848.836 kb on - strand, within Psest_0779 at 849.032 kb on + strand at 849.032 kb on + strand at 849.032 kb on + strand at 849.033 kb on - strand at 849.422 kb on + strand at 849.423 kb on - strand at 849.423 kb on - strand at 849.423 kb on - strand at 849.458 kb on - strand at 849.480 kb on + strand at 849.480 kb on + strand at 849.481 kb on - strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.525 kb on - strand at 849.525 kb on - strand at 849.616 kb on + strand at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.764 kb on - strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.827 kb on - strand, within Psest_0781 at 849.827 kb on - strand, within Psest_0781 at 849.865 kb on + strand, within Psest_0781 at 849.865 kb on + strand, within Psest_0781 at 849.934 kb on + strand, within Psest_0781 at 849.934 kb on + strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.977 kb on - strand, within Psest_0781
Per-strain Table
Position Strand Gene LocusTag Fraction Def Nalidixic 0.001 mg/ml remove 846,885 + +1.1 846,885 + -0.6 846,973 - Psest_0778 0.12 +0.9 846,973 - Psest_0778 0.12 +1.1 847,020 - Psest_0778 0.19 +0.3 847,230 + Psest_0778 0.46 -0.7 847,230 + Psest_0778 0.46 +0.9 847,230 + Psest_0778 0.46 +0.7 847,231 - Psest_0778 0.46 +0.8 847,375 + Psest_0778 0.65 -0.0 847,671 + -1.0 847,671 + +0.4 847,671 + +0.6 847,671 + -2.0 847,671 + +0.2 847,672 - +0.6 847,672 - -2.0 847,672 - +0.3 847,836 + +0.6 847,836 + -0.6 847,837 - +1.7 847,929 + -0.0 847,929 + -0.4 847,929 + +1.2 847,929 + -1.0 847,929 + +0.4 847,930 - +1.3 847,959 - Psest_0779 0.10 +0.8 847,983 + Psest_0779 0.12 -1.8 847,983 + Psest_0779 0.12 +1.0 847,983 + Psest_0779 0.12 +1.2 847,984 - Psest_0779 0.12 -2.0 848,584 - Psest_0779 0.63 +0.7 848,584 - Psest_0779 0.63 +1.6 848,835 + Psest_0779 0.84 +0.8 848,835 + Psest_0779 0.84 +1.6 848,836 - Psest_0779 0.84 -1.0 849,032 + +0.6 849,032 + -1.2 849,032 + -0.9 849,033 - -1.4 849,422 + -1.2 849,423 - +0.6 849,423 - -0.7 849,423 - +0.2 849,458 - -0.0 849,480 + -0.6 849,480 + -0.8 849,481 - -1.4 849,524 + +1.8 849,524 + +0.6 849,524 + +0.7 849,524 + +1.8 849,524 + +0.6 849,525 - +1.6 849,525 - +0.6 849,616 + -0.2 849,736 + Psest_0781 0.15 +0.5 849,736 + Psest_0781 0.15 +0.2 849,736 + Psest_0781 0.15 -0.9 849,736 + Psest_0781 0.15 +2.1 849,736 + Psest_0781 0.15 -0.2 849,737 - Psest_0781 0.16 +0.3 849,737 - Psest_0781 0.16 -1.8 849,737 - Psest_0781 0.16 -1.4 849,737 - Psest_0781 0.16 +1.3 849,764 - Psest_0781 0.19 -1.4 849,826 + Psest_0781 0.27 +0.8 849,826 + Psest_0781 0.27 -0.4 849,826 + Psest_0781 0.27 -1.0 849,826 + Psest_0781 0.27 -0.8 849,827 - Psest_0781 0.27 +0.6 849,827 - Psest_0781 0.27 -0.4 849,865 + Psest_0781 0.32 +1.3 849,865 + Psest_0781 0.32 -0.2 849,934 + Psest_0781 0.41 -1.2 849,934 + Psest_0781 0.41 +0.4 849,935 - Psest_0781 0.41 -1.0 849,935 - Psest_0781 0.41 +0.2 849,935 - Psest_0781 0.41 -0.4 849,977 - Psest_0781 0.46 -0.2
Or see this region's nucleotide sequence